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  • corotation

例句与用法

  • The newly molted 5th instar larva of silkworm bombyx mori was infected with the recombinant virus
    另外，通过共转染和筛选纯化获得了携带appa基因的重组家蚕杆状病毒。
  • ( 3 ) in co - revolution disc cavity , heat transfer coefficient which is high on most region of disc surface show that the co - revolution avails the heat transfer on disc surface
    ( 3 )在共转盘腔结构中，大部分区域盘面的换热系数都较高，表明共转状态对盘面的换热有利。
  • To check if human rap 1 gap can form dimer in mammalian cells , pcdna - myc - raplgap ( human ) and pmt2 - ha - raplgap ( human ) were constructed and co - transfected into a14 cells
    为解决这个问题，我们构建tpcdna3llmyc rp1gap和pmtz ha raplgap重组质粒，将其共转染入ai4细胞。
  • We also conducted the co - transfection with the rnai plasmids rhe and rhc and the selective marker gfp expressing plasmids simultaneously . there are three kinds of combination probability that could be observed by gfp marker
    在实验中同时共转染人集缩素smc亚基hcap一e和hcap一c特异的rnai质粒rhe和rhc和pcdna3 . 1 + kg到hela细胞。
  • In order to get the soluble recombinant eo protein and inspect the protein expression status convinently , the egfp and eo gene were ligated into baculovirus transfer vector . with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna , and plaque purification in the posttransfection procedure , the pure recombinant baculovirus were harvested , which infected the sf9 cells for amplifying to generate a p - l stock . . in the meantime , the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus . the pi - stock from a pure plaque was used to generate a high liter p - 2 stock , which was determined in liter as 1 . 14 107pfu / ml by performing a plaque assay . when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression , the strong fluorescence was obeserved on the day 3 of postinfection
    此外，为了得到可溶性重组eo蛋白并便于观察重组蛋白的表达情况，我们将egfp基因与eo基因相连插入昆虫杆状病毒转移载体中，与线性杆状病毒dna共转染sf9细胞后通过噬斑纯化得到纯的重组杆状病毒，将其感染sf9细胞制备p1种子液，同时用荧光显微镜观察绿色荧光蛋白的表达情况剔除表达效果差的重组杆状病毒。再用p1种子液感染sf9细胞制备高效价的p2种子液。通过病毒液的梯度稀释和噬斑测定，确定p2种子液的病毒滴度达1 . 14 10 ~ 7pfu ml 。
  • To investigate the consequence of this interaction , aes - rfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with tle1 - gfp fusion protein expression vector . confocal microscopy observation showed that aes could interact with tle1 at the cytomembrane region . moreover , this interaction inhibited the concentration of tle1 into nucleus
    在构建了红色荧光蛋白aes表达载体后，将其与tle绿色荧尤蛋白载体共转染细胞，共聚焦显微镜观察发现这两种分子在胞浆中有共存现象，而且aes的表达可抑制tlei向胞核内的聚积。
  • In this study , the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome , which have the lacz reporter gene , earlier / latter promoters lp2ep2 of fpv , promoters p7 . 5 and p7 . 1 of vaccinia virus , replication unnecessary region of fpv - 017 . following 6 cycles screenings , clonings , purification of blue plaques , detection of pcr and dot - elisa , which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully . this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine , as well as provided substance preparatory for prevention the high mortality gpv
    本研究采用脂质体转染方法，将含有完整gpvh1分离株vp3基因、报告基因lacz 、禽痘病毒早晚期启动子lp2ep2 、痘苗病毒启动子p7 . 5 、 p11和fpv - 017复制非必须区的转移载体质粒psy681vp3lacz与fpv - 017共转染鸡胚成纤维细胞，经6轮蚀斑克隆、筛选、表达， pcr鉴定和dot - elisa检测，证明该重组病毒已构建成功，并获得了遗传性状稳定的鹅细小病毒vp3基因的重组禽痘病毒。
  • Finally , a tranfer vector named as pltk - ha was constructed based on pltk - uni with insertion of ha gene of h3 subtype srv . the pltk - ha and prv bartha - k61 genomic dna were co - transfected into 50 - 70 % confluent vero cells in 6 - cm dishes . based on the expression of lacz gene , recombinant prv were selected and purified by blue - colored virus plaque
    利用脂质体介导的方法，将pltk - ha与prvbartha - k61基因组共转染于亚单层vero细胞，依据报告基因lacz在细胞中的表达，筛选蓝色蚀斑的重组病毒，经数次蚀斑纯化后， pcr鉴定、 western - blot分析。
  • They are combination of co - transfection with rhe and gfp expressing plasmids , co - transfection with rhc and gfp expressing plasmids and co - transfection with plasmids rhe and rhc and gfp expressing plasmids at the same time . whichever the combination may be , we always observed the phenomena of chromatin bridge in co - transfected cells . these results indicate that the phenomena of chromatin bridge should be the characteristic feature of deficiency in the human smc subunits
    这时，存在三种可能的情况，即共转染rhe和pedna3 . 1 * kc 、共转染rhc和pedna3 . 1 + kg 、共转染rhe和rhc和pc洲a3 . 1 + kg ，但都观察到明显的染色质桥现象，进一步证明这种表型是人集缩素中smc亚基的缺陷的特征表型。
  • 更多例句：  1  2  3
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