xho中文是什么意思

• [网络] 徐海鸥

• "xhoes" 中文翻译 :    [网络] 徐海鸥 ; (xho 的复数)
• "xhit" 中文翻译 :    Exhibit 陈列,展示
• "xhort-day plant" 中文翻译 :    短照植物
• "xhinese register of shipping" 中文翻译 :    n.中国船级社
• "xhosa seamount" 中文翻译 :    [地名] 科萨海山 ( 大西洋 )
• "xhhw" 中文翻译 :    [网络] 美标电缆
• "xhosan" 中文翻译 :    [网络] 新山
• "xhf" 中文翻译 :    [=Xinjiang hemorrhagic fever]新疆出血热
• "xhosaness" 中文翻译 :    [网络] Xosaness
• "xha" 中文翻译 :    SPECIAL HANDLING SERVICE FOR AIRCRAFT 航空器地面服务门

• Inserted pol ft cdna fragment was recovered from the recombinant vector by xho i and nhe i digesting, and purified
  i和he消化收回在上下游分别有ho11和wb。他性末端的polpcdna片段。
• According to the mutliclone site of pcdnas plasmid, we have used xho i to cut pcdnas plasmid and make its linearized
  pcdna3和doc一ir的酶切片段25经毛dna连接酶作用形成重组质粒。
• A 358 bp pol ft cdna fragment framed with introduced restrict sites of xho i ( upstream ) and nhe i ( downstream ) was amplified by the method of rt-pcr, and then cloned into a ta-vector
  rt一pcr扩增得到358hp的pcilpcdna片段，并在其上下游引物中分别引人了h。i和ie内切酶位点。l此片段克隆人ta克隆载体后，经h。
• Cloning the gene into a pgex expression vector clone the hbrp gene using pcr, restriction digest the pgex-5x-1 vectors and pcr product with bamh i and xho i, recovery the pcr product and pgex-5x-1 vectors
  纯化样品样品上柱，pbs洗柱后，用洗脱buf企r将蛋白洗脱下来。利用蛋白收集器，收集蛋白，浓缩结果1
• Reconstruction of antisense pcdnas-doc-1r plasmid after transformation the pmeiss-fl3-doc-1r plasmid into e . coli cells, we have extracted the plasmid and cut doc-1r fragment using xho i to get a 975 bp doc-1r cdna fragment
  pcdna3-doc-1r反义重组质粒的构建将pme18s-fl3-doc-1r质粒转化后进行质粒提取。用xhoi限制性内切酶从克隆载体上切取975bp的doc-1rcdna片段。
• The plasmid was tested by the restriction enzyme bamh i and xho i incision, and 1 % agarose gel electrophoresis . the results show that there were two bands at the respected sites about 4.9kb and 750bp respectively . it means that hbrp has been cloned into a pgex-5x-1 expression vector correctly
  2.质粒鉴定pgex一hbrp融合蛋白表达质粒转化eoh.bi21感受态细胞l)限制性内切酶鉴定提取质粒dna，经bamhfxhol双酶切鉴定，结果可见，有750bp的插人片段和4.gkb的质粒dna两条带，表明所提质粒中含有外源基因hbrp片段，大小及插人方向均正确。
• After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit . the segment was ligated with vector pmd18-t and then was tranformed into the competent cell of dh5 a . a construction mstnd-pmd18t was generated by inserting the sequence of 254bp into pmd18-t vector and selecting the sense clones . positive clone was identified by three ways : endonuclease digestion, pcr and sequencing . the result showed that the cloned sequence coincides with the designed sequence . this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit . the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ) . after 12-16 hours of culture, several colnes appeared on the plate . some positive clones were selected to extract their plasmid . these plasmids were digested by nco i and xho i and indentified by pcr . a contraction, mstnd-pet28a was generated . the result showed that the cloned sequence coincides with the designed sequence
  f_1长38bp，r_1长36bp，其它片段均40bp长，f_1和r_1片段两端分别加上限制性内切酶nco和xho的识别位点序列。用成对单链片段进行延伸反应，然后用其他单链片段作为引物，进行pcr扩增，用dna快速纯化回收试剂盒回收所得254bppcr产物，与pmd18-t载体连接、转化dh_5。受体菌感受态细胞，利用蓝白斑遗传学筛选法筛选阳性克隆，提取其质粒，采用nco和xho双酶切鉴定，获得了254bp的片段；用pmd18-t载体上的特异引物rv-m和m13-47进行pcr鉴定，获得300bp的片段。
• After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit . the segment was ligated with vector pmd18-t and then was tranformed into the competent cell of dh5 a . a construction mstnd-pmd18t was generated by inserting the sequence of 254bp into pmd18-t vector and selecting the sense clones . positive clone was identified by three ways : endonuclease digestion, pcr and sequencing . the result showed that the cloned sequence coincides with the designed sequence . this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit . the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ) . after 12-16 hours of culture, several colnes appeared on the plate . some positive clones were selected to extract their plasmid . these plasmids were digested by nco i and xho i and indentified by pcr . a contraction, mstnd-pet28a was generated . the result showed that the cloned sequence coincides with the designed sequence
  f_1长38bp，r_1长36bp，其它片段均40bp长，f_1和r_1片段两端分别加上限制性内切酶nco和xho的识别位点序列。用成对单链片段进行延伸反应，然后用其他单链片段作为引物，进行pcr扩增，用dna快速纯化回收试剂盒回收所得254bppcr产物，与pmd18-t载体连接、转化dh_5。受体菌感受态细胞，利用蓝白斑遗传学筛选法筛选阳性克隆，提取其质粒，采用nco和xho双酶切鉴定，获得了254bp的片段；用pmd18-t载体上的特异引物rv-m和m13-47进行pcr鉴定，获得300bp的片段。
• The ns1 and hns2 gene from aiv ( h9n2 ) isolate were cut from the pmd18-t-nsl vector, the ns1 gene cloned into the sites of bamh i and xho i of prokaryotic expression vector and the hns2 cloned into the sites of ecor i and xho i of prokaryotic expression vector . the recombinant vector was identified by endonuclease
  从含有禽流感病毒分离株非结构蛋白基因的重组质粒pmd18-t-ns1中切取ns1、hns2基因片段，分别将其亚克隆于pgex-6p-1的bamh、xho位点和ecor、xho位点上，然后经单、双酶切及质粒pcr鉴定，结果表明目的片段基因成功插入表达载体中。
• The ns1 and hns2 gene from aiv ( h9n2 ) isolate were cut from the pmd18-t-nsl vector, the ns1 gene cloned into the sites of bamh i and xho i of prokaryotic expression vector and the hns2 cloned into the sites of ecor i and xho i of prokaryotic expression vector . the recombinant vector was identified by endonuclease
  从含有禽流感病毒分离株非结构蛋白基因的重组质粒pmd18-t-ns1中切取ns1、hns2基因片段，分别将其亚克隆于pgex-6p-1的bamh、xho位点和ecor、xho位点上，然后经单、双酶切及质粒pcr鉴定，结果表明目的片段基因成功插入表达载体中。