xho造句

• Inserted pol ft cdna fragment was recovered from the recombinant vector by xho i and nhe i digesting, and purified
  i和he消化收回在上下游分别有ho11和wb。他性末端的polpcdna片段。
• According to the mutliclone site of pcdnas plasmid, we have used xho i to cut pcdnas plasmid and make its linearized
  pcdna3和doc一ir的酶切片段25经毛dna连接酶作用形成重组质粒。
• A 358 bp pol ft cdna fragment framed with introduced restrict sites of xho i ( upstream ) and nhe i ( downstream ) was amplified by the method of rt-pcr, and then cloned into a ta-vector
  rt一pcr扩增得到358hp的pcilpcdna片段，并在其上下游引物中分别引人了h。i和ie内切酶位点。l此片段克隆人ta克隆载体后，经h。
• Cloning the gene into a pgex expression vector clone the hbrp gene using pcr, restriction digest the pgex-5x-1 vectors and pcr product with bamh i and xho i, recovery the pcr product and pgex-5x-1 vectors
  纯化样品样品上柱，pbs洗柱后，用洗脱buf企r将蛋白洗脱下来。利用蛋白收集器，收集蛋白，浓缩结果1
• Reconstruction of antisense pcdnas-doc-1r plasmid after transformation the pmeiss-fl3-doc-1r plasmid into e . coli cells, we have extracted the plasmid and cut doc-1r fragment using xho i to get a 975 bp doc-1r cdna fragment
  pcdna3-doc-1r反义重组质粒的构建将pme18s-fl3-doc-1r质粒转化后进行质粒提取。用xhoi限制性内切酶从克隆载体上切取975bp的doc-1rcdna片段。
• The plasmid was tested by the restriction enzyme bamh i and xho i incision, and 1 % agarose gel electrophoresis . the results show that there were two bands at the respected sites about 4.9kb and 750bp respectively . it means that hbrp has been cloned into a pgex-5x-1 expression vector correctly
  2.质粒鉴定pgex一hbrp融合蛋白表达质粒转化eoh.bi21感受态细胞l)限制性内切酶鉴定提取质粒dna，经bamhfxhol双酶切鉴定，结果可见，有750bp的插人片段和4.gkb的质粒dna两条带，表明所提质粒中含有外源基因hbrp片段，大小及插人方向均正确。
• After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit . the segment was ligated with vector pmd18-t and then was tranformed into the competent cell of dh5 a . a construction mstnd-pmd18t was generated by inserting the sequence of 254bp into pmd18-t vector and selecting the sense clones . positive clone was identified by three ways : endonuclease digestion, pcr and sequencing . the result showed that the cloned sequence coincides with the designed sequence . this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit . the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ) . after 12-16 hours of culture, several colnes appeared on the plate . some positive clones were selected to extract their plasmid . these plasmids were digested by nco i and xho i and indentified by pcr . a contraction, mstnd-pet28a was generated . the result showed that the cloned sequence coincides with the designed sequence
  f_1长38bp，r_1长36bp，其它片段均40bp长，f_1和r_1片段两端分别加上限制性内切酶nco和xho的识别位点序列。用成对单链片段进行延伸反应，然后用其他单链片段作为引物，进行pcr扩增，用dna快速纯化回收试剂盒回收所得254bppcr产物，与pmd18-t载体连接、转化dh_5。受体菌感受态细胞，利用蓝白斑遗传学筛选法筛选阳性克隆，提取其质粒，采用nco和xho双酶切鉴定，获得了254bp的片段；用pmd18-t载体上的特异引物rv-m和m13-47进行pcr鉴定，获得300bp的片段。
• After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit . the segment was ligated with vector pmd18-t and then was tranformed into the competent cell of dh5 a . a construction mstnd-pmd18t was generated by inserting the sequence of 254bp into pmd18-t vector and selecting the sense clones . positive clone was identified by three ways : endonuclease digestion, pcr and sequencing . the result showed that the cloned sequence coincides with the designed sequence . this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit . the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ) . after 12-16 hours of culture, several colnes appeared on the plate . some positive clones were selected to extract their plasmid . these plasmids were digested by nco i and xho i and indentified by pcr . a contraction, mstnd-pet28a was generated . the result showed that the cloned sequence coincides with the designed sequence
  f_1长38bp，r_1长36bp，其它片段均40bp长，f_1和r_1片段两端分别加上限制性内切酶nco和xho的识别位点序列。用成对单链片段进行延伸反应，然后用其他单链片段作为引物，进行pcr扩增，用dna快速纯化回收试剂盒回收所得254bppcr产物，与pmd18-t载体连接、转化dh_5。受体菌感受态细胞，利用蓝白斑遗传学筛选法筛选阳性克隆，提取其质粒，采用nco和xho双酶切鉴定，获得了254bp的片段；用pmd18-t载体上的特异引物rv-m和m13-47进行pcr鉴定，获得300bp的片段。
• The ns1 and hns2 gene from aiv ( h9n2 ) isolate were cut from the pmd18-t-nsl vector, the ns1 gene cloned into the sites of bamh i and xho i of prokaryotic expression vector and the hns2 cloned into the sites of ecor i and xho i of prokaryotic expression vector . the recombinant vector was identified by endonuclease
  从含有禽流感病毒分离株非结构蛋白基因的重组质粒pmd18-t-ns1中切取ns1、hns2基因片段，分别将其亚克隆于pgex-6p-1的bamh、xho位点和ecor、xho位点上，然后经单、双酶切及质粒pcr鉴定，结果表明目的片段基因成功插入表达载体中。
• The ns1 and hns2 gene from aiv ( h9n2 ) isolate were cut from the pmd18-t-nsl vector, the ns1 gene cloned into the sites of bamh i and xho i of prokaryotic expression vector and the hns2 cloned into the sites of ecor i and xho i of prokaryotic expression vector . the recombinant vector was identified by endonuclease
  从含有禽流感病毒分离株非结构蛋白基因的重组质粒pmd18-t-ns1中切取ns1、hns2基因片段，分别将其亚克隆于pgex-6p-1的bamh、xho位点和ecor、xho位点上，然后经单、双酶切及质粒pcr鉴定，结果表明目的片段基因成功插入表达载体中。
• In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv . the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem-t easy vector . after transforming e . coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr . presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem-3abc . comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent . the pgem-3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii-digested expression vector ptriex-4 neo . lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene . positive clones were selected and induced with lmmol / l isopropyl-d-galactoside ( iptg ), bacteria were detected by sds-page and western blotting after properly treated . the results showed that the 3ab gene expressed successfully in e . coli and 33.5ku fusion protein can be recognized by the positive bovine serum of fmdv . the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
  扩增产物连接到pgem-teasy载体中，转化大肠杆菌dh5菌株，筛选氨苄青霉素抗性菌落，提取质粒经酶切鉴定、pcr分析以及确证性测序证明，所克隆的1500bp左右的片段含有完整的3abc基因，与国外参考序列相比，同源性在99以上。将重组质粒pgem-3abc和表达载体ptriex-4neo分别用sal和bgl与xho和bgl消化后，亚克隆3abc基因至原核表达载体ptriex-4neo中，通过酶切鉴定、pcr扩增以及序列分析，发现克隆到ptriex-4neo载体上的片段于3abc基因708bp处出现了17bp的缺失，碰巧在3ab基因后形成一终止密码子，但3ab基因的阅读框架完整，选出含有3ab基因完整阅读框架的阳性克隆，用iptg诱导表达，收集菌液进行sds-page电泳、westernblotting分析，结果表明，3ab基因在大肠杆菌中成功表达，其表达产物为分子量33.5ku的融合蛋白，并能被口蹄疫病毒阳性血清识别。经薄层扫描分析，表达量占总蛋白量的26以上。
• A gus report gene, gained by the method of pcr, was inserted into the pet22b vector between the sites of hindiii and xho i to construct a transitional vector named pet22g then the signal peptide ( pelb leader ) in the pet22g vector was replaced by a containing grb-ast signal peptide fragment to construct expression vector pet22asg which had been transformed into expression host e . coli bl21 ( de3 ) plyss
  先将pcr获得的gus报告基因插入到经hind和xho双酶切的pet22b中，构建了中间载体pet22g；然后再将pcr克隆的一含ast信号肽的片段as取代pet22g上的信号肽pelbleader序列，构建了大肠杆菌分泌表达载体pet22asg。
• Materials and methods in our study, first the e . coli bl21 transformed already are cultivated in smaller scale and then the plasmids dna were extracted by the methods of alkaline lysis . the plasmids dna extraction were processed 37 overnight by the restrictive endo-incisase bam h i and xho i . the incision products were used to check the integrality and variability of the recombinant plasmids dna by 1 % agarose gel elec-trophoresis
  同预想结果基本吻合，大规模培养后纯化得到的融合蛋白通过sdspage显示在52kda处有一条带，而酶切后产物电泳结果显示在26kda处分另有两条蛋白带，其中一条为gst，另一条为hbrp，基本符合实验结果；hbrp对tpk活性的抑制呈剂量依赖性。