The results suggested : the differential gene expression in the adrenal gland under acute hyperglycemia / hypoglycemia was related to blood glucose ; these genes might regulate blood glucose by influencing the metabolism and secretion of hormones , cytoskeleton constitution and signal transduction in the adrenal gland ; bbs4 gene might participate in glucoregulation via the activities of adrenal medulla ; the differential genes expressed under acute vs chronic hyperglycemia might play a basic role of the structural and functional changes in the adrenal gland , lead to the process of chronic complications of diabetes or accommodate to the status of hyperglycemia 结果提示：肾上腺基因差异性表达和血糖变化有关；在急性高低血糖情况下，这些基因可能通过影响肾上腺内激素的分泌和代谢、细胞骨架构成和信号转导来调节血糖； bbs4可能参与血糖调节，其作用可能和肾上腺髓质的活动有关；慢性高血糖情况下的肾上腺差异表达不同于急性高血糖，这种表达差异可能与肾上腺慢性的结构和机能改变相关，或参与了糖尿病慢性并发症的发生发展、高血糖状态的调节。
The results from rt - pcr of the three differentially expressed genes were significantly higher than those of control group ( p < 0 . 01 ) in summary , with rapidness and effectiveness of ssh technique , the screened differentially expressed genes including the known and three novel ones in this study might play an important role in the pathogenesis of rat brain injury induced by repeated + gz exposures 选取的3个差异表达基因用半定量rl 、 pcr检定结果表明，各基因在对照组和暴露组中的表达水平有明显差异( p < 0 . 01 ) 。上述研究结果提示，应用抑制性消减杂交这一筛选差异表达基因的快速有效工具所发现的3个新的cdna ，可能在+ gz脑损伤的病理过程中起重要作用。
In this study , sd rats were used to establish the animal model of brain injury induced by repeated + gz exposure and suppression subtractive hybridization technique was adopted to screen the differentially expressed genes in rat brains of + gz exposure group . the aim of the study was to obtain preliminary experimental data for the molecular mechanisms of the brain injury 本研究利用大鼠重复+ 10gz暴露引发脑损伤的动物模型，观察脑的病理学改变；应用抑制性消减杂交技术筛选+ gz重复暴露大鼠脑的差异表达基因，旨在初步探讨+ gz重复暴露致脑损伤的分子机制。
The results indicated : 1 ) 521 genes up - regulated and 92 genes down - regulated under acute hyperglycemia , while 294 genes up - regulated and 464 down - regulated under acute hypoglycemia ; 1208 genes expressed differentially between acute hyperglycemia and hypoglycemia , including 94 genes expressed bi - directionally ; 2 ) bbs4 gene , which mainly expressed in the adrenal medulla , was up - regulated by hyperglycemia and down - regulated by hypoglycemia ; 3 ) 79 genes up - regulated and 43 down - regulated under chronic hypoglycemia , 725 genes expressed differentially between acute and chronic hyperglycemia , including 2 genes expressed bi - directionally 结果显示： ( 1 )在急性高血糖情况下有521个基因上调， 92个下调，急性低血糖情况下294个上调， 464个下调；差异表达的基因共1208个，其中双向性表达基因94个； ( 2 )在急性高低血糖情况下， bbs4基因呈双向性表达，原位杂交发现其主要表达在肾上腺髓质； ( 3 )慢性高血糖情况下， 79个基因上调， 43个下调，差异表达基因725个，其中双向性表达基因2个。
Part 2 cloning and analysis of the full - length cdnas of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) chapter 1 construction of race cdna library of mitten crab ( eriocheir japonica sinensis ) ovary in order to get full - length cdna sequences of the differentially expressed genes , a race cdna library of the mitten crab ovary at stage iii was constructed successfully with smart technique 南京师范大学博士研究生毕业论文第二部分中华绒螫蟹卵巢发育相关基因全长叨a的克隆及序列分析工中华绒蟹蟹卵巢ra kc a文库的构建为了获得差异表达基因的全长cdna序列，运用smart技术，构建了中华绒蟹蟹卵巢（ ill期） racecdna文库。
All of these indicated that arsenic trioxide was a powerful chemotherapeutic agent and the cells in the treatment group were induced to apoptosis . to further understand the molecular mechanisms of arsenic trioxide treatment in the induced cellular apoptosis , we applied the restriction display - pcr ( rd - pcr ) technique combined with polyacrylamide gel electrophoresis and sliver staining techniques to separate the differentially expressed genes 为了进一步阐述as _ 2o _ 3作用的分子生物学机制，我们首先应用rd - pcr技术，结合聚丙烯酰胺凝胶电泳和银染技术分离和显示了as _ 2o _ 3作用k562细胞前后的cdna片段，发现了11个差异表达的基因片段。
