Proteomic analysis was performed by two - dimensional protein gel electrophoresis and subsequent computerized gel analysis for detection of distinguishing patterns of protein expression 蛋白质组学分析是由二维蛋白凝胶电泳和随后的计算机辅助凝胶分析并发现差异表达的蛋白。
Methods dna chip was used to detect the mrna from 11 human transitional cell carcinoma tissues and to investigate the genes related to signal transduction 方法使用人肿瘤基因表达谱芯片检测11例膀胱移行细胞癌组织基因表达谱的变化，以寻找与细胞信号转导相关的差异表达基因。
The result was photographed under uv light and analyzed with a quantification system . the ratio of differentially expressed genes / gapdh was determined to eliminate gel - to - gel variance 为消除系统及定量误差，差异基因的表达水平以相对表达量即差异表达基因/ ga卫dh的比率来计算。
This simple , yet powerful technique provides essentially complete screening of the mrna pools of related cell types for qualitative and quantitative differences in specific mrna species 讨论1992年，梁鹏和pardee建立了一种新的显示mrna差异表达的技术称为mrna差异显示或差异显示反转录pcr 。
Further analysis revealed 6 genes were involved in the apoptosis pathway . we also found in our study that igfs may also play a role in the arsenic trioxide induced apoptosis 进一步分析发现有6个差异表达基因与as _ 2o _ 3的作用密切相关，并分别位于as _ 2o _ 3抑制细胞生长、诱导细胞凋亡的不同通路中。
Sequence analyses for those limited amount of inserts indicated that , ( 1 ) a few particular gene populations are expressed in d . involucrata sprouting bract , e . g . , 分析本试验获得的烘桐苞片差异表达的有限几个部分或全长序列的cdna发现： ）处于生长发育初期的苞片中有一些较为独特的基因表达。
We utilized microarray and real - time quantitatie polymerase chain reaction ( pcr ) to identify genes differentially expressed at 6 h reperfusion in periinfarct cortex from castrated rats with or without dht replacement 我们利用微点阵和实时定量pcr方法在再灌注6小时时，对进行/未进行dht替代的阉割大鼠梗死周围皮层的基因差异表达进行了鉴定。
After the second pcr amplification , 10 differential cd na fragments were identified among which 6 fragments were wild type specific and 4 fragments were ast mutant specific . all of the 10 differential cdna fragments were sequenced 二次pcr扩增后，进一步筛选出10个差异表达的cdna条带，其中6个是野生型特异表达的， 4个是突变型特异表达的。
We utilized microarray and real - time quantitative polymerase chain reaction ( pcr ) to identify genes differentially expressed at 6 h reperfusion in periinfarct cortex from castrated rats with or without dht replacement 我们利用微点阵和实时定量pcr方法在再灌注6小时时，对进行/未进行dht替代的阉割大鼠梗死周围皮层的基因差异表达进行了鉴定。
With the modification of the method , the technique of differential display ( dd ) has become a very useful method for elucidating changes in gene expression in response to temporal de - velopment , disease and mitogenic stimulants 在过去几年里，出现了许多技术方面的改进，主要集中在增加对于差异表达基因鉴定的效率和降低对于基因鉴定的工作量两个方面。
