差异表达

• In the past , we cloned the cdna of zmcdc5 based on the studies on difference of gene expression in shoot and radicle of maize seedling in our lab and then got the whole genome sequence by maize genomic library screening and inverse pcr
  本实验室根据玉米胚芽和胚根差异表达的研究，克隆得到zmcdc5基因的cdna ，并通过筛库和反向pcr获得了zmcdc5基因组全长序列。
• Chapter 3 sequencing cdna fragments of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) partial clones were sequenced which were selected from the differentially expressed clones screened by cdna macroarray analysis
  3中华绒螯蟹卵巢发育相关基因cdna片段的序列测定从cdna微阵列筛选的差异表达克隆中选取部分克隆进行测序。
• Northern blot hybridization showed that leerfl , leerf2 and pti4 ( a member of erebps in tomato ) expressed differentially in different parts of wild tomato fruit during ripening , which was related with fruit ripening
  Northern杂交分析表明： leerf1 、 leerf2 、 pti4 (已发表的番茄erebps成员)基因在普通番茄果实的成熟过程及果实的不同部位都有差异表达，表现出与成熟进程的相关性。
• In order to study the salt tolerance mechanism at the expression level , our laboratory made the expression gene chips of dunalliena salina after high salt shock . a series of sequences were screened which express differently under distinct salt conditions
  为了在基因表达水平上研究其耐盐机制，本实验室制作了盐藻在高渗震荡下的表达谱基因芯片，从中筛选到了一系列高渗震荡下差异表达的序列。
• Blastn analysis showed that each of the 10 sequences had no homology with the structural genes or regulatory genes in the anthocyanin biosynthesis . it was suggested that there were some limitations to isolate the specific ast gene by ddrt - pcr
  Blastn分析表明，这10个差异表达的cdna片段与数据库中花青苷生物合成途径中的结构基因和调节基因序列没有同源性，表明用ddrt - pcr的方法克隆特定的ast基因有一定的局限性。
• Sh - sy5y cells were differentiated into neuron - like cells by ra treatment in low concentration , total rna was extracted and labeled to hybridize with the microarray , the changes on gene expression were detected to find differentially expressed genes
  首先应用低浓度的ra处理sh - sy5y细胞，促使细胞分化成神经元表型，然后提取总rna并进行标记，与制备的基因表达谱芯片进行杂交，检测基因表达变化，查找差异表达基因。
• To further confirm the expression levels of those differentially expressed genes in rat brain of + gz exposure group , the primers for two known genes ( 53 # and 72 # ) and one unknown genes ( 58 # ) were designed for semi - quantitative reverse transcription polymerase chain reation ( rt - pcr ) , using gapdh as the reference
  4 、进一步验证了通过ssh方法得到的差异表达基因片段在+ 1ogz重复暴露脑组织中的差异表达情况。对两个己知基因克隆53 # 、 72 #及一个新基因克隆58 #分别设计引物，以gapdh为内参照，进行半定量rl 、 pcr反应。
• Among sequenced 16 positive clones randomly selected , two represent novel expression tag sequences , two are homologous to two unknown proteins in genbank ; the rest are homologous to known or putative proteins or enzymes with definite functions by searching the genbank through blast program , which are involved in various life activities of cell such as regulation of gene expression , plant secondary metabolism , signal transduction , adversity resistance , stress response and defense reaction . significant changes of quantities of these gene fragments were observed before and after ssh , which indicated they were enriched after ssh
  随机挑选了16个差异表达的克隆进行序列测定，经与genbank数据库相关数据比较分析，发现有2个新的cdna片段( ets ) ，有12序列与genbank中已知蛋白或推测蛋白质( putativeprotein )序列有高低不同的同源性，它们参与基因的表达调控、植物的次生代谢、信号传导、抗逆、应激及防御反应等细胞生命活动过程。
• In this paper , our method was performed in two separate steps . first , microarray data was normalized to eliminate experiment - wide systematic effects and then differentially expressed genes were prejudged under a loose standard via a single gene model . second , these differentially expressed genes were confirmed under a stricter standard to control the false positive via a multi - gene model
  本文提出的方法主要分为两步来进行：首先，将芯片数据通过噪音过滤消除大的试验系统误差，然后在一个比较宽松的标准下通过单基因模型初步判断差异表达基因；其次，用多基因模型分析这些初定的差异表达基因以便在较严的标准下控制假阳性。