To identify the interaction between kyot2 and zo - 2 - i3 , yeast two - hybrid system , purification of kyot2 protein and gst pull - down assay were performed in the experiments . after kyot2 and zo - 2i3 exchanged their vectors , yeast two - hybrid test revealed physical binding of the two proteins 本实验通过酵母双杂交及gstpull downas脚验证了kyotz蛋白与zoz蛋白在体外的相互作用，并通过酵母双杂交实验初步确定其相互作用的位点。
The yeast two - hybrid system is an effective method for the study of protein - protein interaction in recent years . it is widely used in the study of life science because it provides a genetic means of identifying proteins that physically interact in vivo 酵母双杂交系统是近年来发展起来的一种研究蛋白质?蛋白质相互作用的有效方法，由于它检测的是一种体内的相互作用，自这一系统建立以来，就被广泛地应用于生命研究的各个领域。
Even so , by truncating hbv pres gene , we finally obtained some useful " " bailors " , either nontoxic or self - activating , and used them to fish dna fragments of hbv pres interacting protein ( s ) from an ad vector constructed human embryonic cdna library 我们通过第回军巨大学硕士学位论文对pres基因分段截短的方法，获得了对酵母细胞即无毒性作用，又没有自激活作用的“诱饵” ，通过它在酵母双杂交系统中筛选构建于ad载体的人胎肝。
Using yeast two - hybrid system with lim domain protein kyotl as a bait , we have isolated an alternatively spliced form of human tight junction protein 2 - - - - - - zo - 2 - i3 . the tight junction ( or zonula occludens ) is the most apical junction between neighbouring epithelial cells or endothelial cells 53为深入研究kyoth在notch rbpjk途径中的可能作用及其作用机制，本实验室以kyoth为诱饵蛋白通过酵母双杂交得到人类紧密连接蛋白zoe 。
We obtained two positive clones after screening . sequencing and blasting results showed that the two clones had the same sequences and encoded part of ked like protein in arabidopsis . the coding region was from n terminal amino acid 240 to c terminus 测序及序列比对结果表明二者序列相同，为ked样蛋白的部分编码序列，编码部分从n端第240位氨宋林夜:利用酵母双杂交系统筛选与g蛋白及cam相互作用的新型蛋白基酸至c末端，共207个氨基酸。
The results showed that all six tfs displayed no autoluminescence in this two - hybrid system , and one of them , the atf3 displayed a strong interaction with restin . to verify the interaction of restin and atf3 , a series of truncated fragments of atf3 were inserted into pact to be used in the further study 三、为了进一步验证atf3与restin的相互作用，对atf3进行截断并构建了一系列atf3的截短片段的双杂交质粒(仍然构建于pact载体中) ，再次运用细胞双杂交进行相互作用的探索。
The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga . - galactosidase assay indicated that got did not have the property of self - activation . after pgbkt7 - ga was transformed to yeast pj69 - 4a , we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga 通过pcr扩增得到gpa1基因并将其克隆到双杂交dna结合域载体pgbkt7中，得到的重组质粒命名为pgbkt7 - g ， ?半乳糖苷酶活性鉴定表明g不具有自激活特性，将其转化到酵母菌pj69 - 4a中，再以此为受体菌转化拟南芥绿色营养组织cdna文库质粒。
At first , total 31 transcription factors correlated to cell proliferation , differentiation , physiological stress and apoptosis , were selected ( see in article ) to use in the two - hybrid , and cloned into plasmid pact by using rt - pcr to amplify these transcription factors from fetal tissue cdna or stimulated lymphocyte cdna . as result , only six transcription factors atf3 , atf4 , e2f6 , c - jun , c - fos and p53 were correctly cloned 基于以上的背景信息，本文利用细胞双杂交技术，对resrin在细胞周期中可能的作用的转录因子进行了筛选:一、首先从genebank中筛选了31个与细胞周期密切相关的转录因子，设计了特异性引物。从胎儿组织和刺激后的淋巴细胞中克隆了这些转录因子并构建于细胞双杂交系统的pact质粒中。
The result showed that both ldplcl and ldplc2 can physical interacted with gp , but neither ga nor g in addition , arabidopsis plc knock - out and overexpression lines were also characterized , and the result proved that lose of function of atplc ( at3g55940 ) induced atplc ( at3g08510 ) gene expression and increase pollen germination rate , whereas gain of function of atplc ( at3g55940 ) repressed atplc ( at3g08510 ) expression , and decrease pollen germination 本文还利用酵母双杂交体系检验了plc与g蛋白各亚基的相互作用，发现ldplci与gq和gy的杂交结果为阴性，与gp亚基可发生相互作用; ldplcz与gp弱相互作用，与其他亚基没有作用。上述结果表明在g蛋白和plc的相互作用中， p亚基可能起调节作用。
Using yeast two - hybrid system honjo ' s group in kyoto university isolated a novel protein , kyot , which physically interacts with rbp - j . differential splicing gives rise to three transcripts of the kyot gene , kyotl , kyot2 and kyots . kyot2 , but not kyotl negatively regulates transcription by association with rbp - j 为进一步探讨rbp - j的功能，日本京都大学的honjo研究组利用酵母双杂交系统筛选到一个可以与rbp - j相互作用的新蛋白kyot ， kyot蛋白有三种不同的剪切体： kyot1 ， kyot2和kyot3 ，其中kyot2可以与rbp - j相互作用抑制其转录活性。
