Metallothionein - 3 , previously named as growth inhibitory factor , is the unique member of the metallothionein family that demonstrates bioactivity in vitro . to test if mt - 3 dimerizes or polymerizes itself 为了验证其在细胞内是否形成二聚或多聚体，构建酵母双杂交系统用于检测。
Further we cloned full - length cdna of the ked like protein coding region via pcr amplification and confirmed its interaction with acam2 in yeast two - hybrid system 进一步通过rt . pcr克隆了ked样蛋白编码区全长cdna ，通过双杂交验证了全长ked样蛋白与acamz的相互作用，结果为阳性。
Pias1 , a protein that was also located in test ' s specifically was got pdz domain protein tight junction protein 2 and pcg ( polycomb group ) family ring1 and polycomb 2 were included 3kbp （ kyotbindingpotcin ）功能的研究： kbp是我们通过酵母双杂交获得的与kyo ’ fz相互作用的一种新分子。
We cloned full - length cdna of ca coding region via pcr amplification , but a negative result was obtained when the interaction of full - length ca and ga was tested in yeast two - hybrid system 通过rt - pcr克隆了ca编码区全长cdna ，但是在双杂交系统检测全长ca与g蛋白的相互作用时，结果为阴性。
In order to study the upstream and downstream components in g protein and calmodulin signal pathways , a yeast two - hybrid system was used to screen interaction partners of these two proteins 本文利用酵母双杂交系统分别筛选了与这两种蛋白相互作用的蛋白质，以进一步研究g蛋白和cam信号转导途径中的其它上下游组分。
In our study , the igii subdomain of trka extracellular domain was fused to pgbkt7 as a bait to screen yeast random peptide library which contains 1 107 independent random peptide clones 我们将trk受体膜外域1911结构域与gal4蛋白融合构建成pgbkt7一ign ，确认无自激活作用后，将其作为诱饵蛋白，洲于酵母双杂交筛选。
To insight the function of raplgap and the mechanism of raplgap hydrolyzing gtp to gdp , yeast two hybrid system ( y2h ) was employed to look for the potential proteins that interact with raplgap protein 为了研究rap1gap的作用机制及rap1gap在信号转导中的作用，我们应用酵母双杂交体系( yeasttwohybridsystem )寻找潜在的与rap1gap相作用的蛋白质。
In order to figure out the machanism of transcription control by ap - 2 a and to find the partner proteins which derectly binding to ap - 2 a , a yeast two - hybrid system by using ap - 2 a as a bait was performed to screen the hela cell cdna library 为了阐明ap - 2确切的转录调控方式，找出与其直接相互作用的蛋白质因子，我们以ap - 2为饵蛋白通过酵母双杂交对hela细胞的cdna文库进行了筛选。
The yeast two - hybrid system was established . in yeast two - hybrid system , mt - 3 interacts with mt - 3 . a further two - hybrid test also indicated that mt - 3 weakly interacted with its isoform mt - 1 . these primary results suggest that mt - 3 probably trend to homodimerize or heterodimerize in vivo 结果表明： mt - 3与mt - 3之间在酵母细胞内能发生相互作用，进一步用酵母双杂交实验还表明mt - 3与mt - 1间也存在弱相互作用。
Then we cloned the mature ca fragment and the same region of the selected ca fragment from library plasmid to vector pgadt7 , but neither did they interact with ga in the assay of two - hybrid system . then we used arabidopsis cam2 isoform as bait to screen the two - hybrid library 之后将成熟ca和文库中筛到的ca编码部分的基因分别亚克隆至双杂交激活域载体pgadt7 ，进行双杂交检测它们与g蛋白的相互作用，结果仍为阴性。
