In this study , the profiles of proteins expesssion in different growth phases of rt19 have been obtained using the technique of proteome analysis with two - dimensional polyacrylamide gel electrophoresis ( 2 - d page ) . an efficient imagemaster 2d was used to reveal the number of protein spots corresponding from lag phase to stationary phase , varying from 398 to 516 , which manifested kinetic state of proteome in cell 本文采用高分辨率的双向电泳技术，得到不同生长时期的蛋白表达谱，应用高效的imagemaster2d软件进行图谱分析，结果显示： ( 1 )从延滞期到稳定期的末期，所表达的蛋白数量由398个逐渐增加至516个，显示了细胞内的蛋白在不同生长期的动态表达水平。
Indentificatiort is also the first step towards studies on protein co - and post - translational modification , and ultimately , function . in the present study , the total proteins of the photo - thermo sensitive genie male - sterile rice ( oryza sativa , peiai64s ) spikelet at meiosis stage were used as the material . by optimizing crucial factors and procedures such as sample treatment , electrophoresis parameters , and gel concentration , 2 - d maps with high quality and reproducibility were obtained 用两种方法对经双向电泳分离的凝胶上的蛋白质点进行了初步鉴定，一是通过电印迹转移把蛋白质转到pvdf膜上，再用edman降解的方法测得部分相对分子质量在10000 - 30000da的蛋白质点的n -端序列，通过网上搜索其同源性对其进行鉴定，并确定该点在凝胶上的位置。
57 protein spots out of about 1000 detectable spots on the 2 - d gels were indentified by the following two methods : l ) n ~ terminal edman degradation microsequencing after the protein spots were electro - transferred to pvdf membrane . 2 ) maldi - tof - ms peptide fingerprint analysis of the protein spots and protein database searching . the 2d protein maps of the rice spikelet during the sterile and fertile stages were compared 二是通过原位酶解，抽提蛋白质片段进行maldi - tof质谱分析，利用肽质量指纹图数据在数据库中进行检索，在双向电泳凝胶上取了57个点进行分析，有34个蛋白质点在数据库得到归属鉴定。
