Two - dimensional electrophoresis , mass spectrometer and bioinformatics are three main techniques of proteomics 双向电泳技术、质谱技术和生物信息学是蛋白质组学的三大支撑技术。
A new method of two - dimensional electrophoresis is introduced , and applied to analyze protein subunits 摘要介绍了还原条件与非还原条件双向电泳法，并应用此方法研究了种子蛋白质亚基结构。
The results show that the new method has been employed to understand the constitution of seed protein subunits in details 实验结果表明，这种双向电泳法能快速而准确地测定种子蛋白质的亚基结构。
Establishment and analysis of serum two - dimensional gel electrophoresis profiles of myasthenia gravis patients with spleen and kidney deficiency syndrome 脾肾虚型重症肌无力患者血清双向电泳图谱的建立及分析
The cdna probe was prepared by pcr amplification technique . colorimetric and chemiluminescent detection for biotinylated probe were used in this test 同时，利用双向电泳和rt - pcr方法进行类病毒鉴定，证明两种方法均可用来检测类病毒。
In order to reveal the mechanisms of extreme radioresistance and dna repair in deinococcus radiodurans , we examined proteome changes in a wild type strain following y - irradiation using 2 - dimensional polyacrylamide gel electrophoresis and silver staining 为了研究耐辐射球菌极强的辐射抗性与dna修复机理，我们应用双向电泳结合银染的方法考察了野生型菌株kd8301在射线照射前后细胞体内总蛋白组的变化情况。
To provide evidence for illustrating the molecular mechanism of pancreas development , proteins were extracted from rat pancreas tissue at various stages , separated by high resolution two - dimensional electrophoresis ( 2 - de ) , and the good 2de pattern including resolution and reproducibility was obtained 本研究利用双向凝胶电泳技术分离sd大鼠成年胰腺组织和胚胎18 . 5天胰腺组织总蛋白，银染和考染显色，获得了分辨率和重复性均较好的双向电泳图谱。
The results from sds - page presented that there were three female specific protein subunits with molecular weights of 123 kd , 120 kd and 91 kd , respectively . we can conclude the higher molecular compose of two subunits ; the results from two dimension electrophoresis showed the isoelectric points of two female - specific spots with molecular weight of about 120kd were 5 . 5 and 5 . 7 . immunodiffusion reactions demonstrated that vg existed both in female fat body and hemolymph , which as vn was deposited in the ovary , while not in the male Page电泳结果表明：丽蝇蛹集金小蜂明显存在2条雌特异性带-卵黄蛋白，分子量分别为181kd和136kd ； sds - page电泳分析：存在3条雌特异性带，其分子量为123kd 、 120kd和91kd ，由此，可推定卵黄原蛋白( vitellogenin ， vg )和卵黄磷蛋白( vitellin ， vn )由2个蛋白组成，其中分子量较大的蛋白由2个亚基组成；双向电泳结果显示，在120kd附近有两个特异性点，其等电点为5 . 5和5 . 7 ；双扩散表明，丽蝇蛹集金小蜂卵黄磷蛋白的抗血清与雌隐成虫虫体、脂肪体、血淋巴和卵巢匀浆液均有免疫沉淀反应，而与雄蜂血淋巴无免疫反应，说明了vg与vn具有免疫同源性，是雌特异性蛋白，且由脂肪体合成。
Methods and results : proteomics approaches involve three critical techniques : two dimensional electrophoresis , biological mass spectrometry and bioinformatics . in 2 - de , we applied traditional ief in the first dimension and tris - tricine system in the second dimension and acquired protein profiles of serum and skin samples . in the identification of 10 proteins of serum and 16 proteins of skin , we acquired peptide mass of fingerprint ( pmf ) maps for all targets by means of matrix - assisted laser desorption - ionization time of flight mass spectrometry ( maldi - tof - ms ) and partial aminio acid sequences for 9 proteins by means of electrospray ionization ms / ms ( lisi - ms / ms ) 研究方法：采用双向电泳技术获取泥鳅创伤前后血清和皮肤的小分子蛋白图谱，利用基质辅助激光解析飞行时间检测质谱分析( matrix - assistedlaserdesorption - ionizationtimeofflightmassspectrometry , maldi - tof - ms )和电喷雾离子化串连质谱分析( electrosprayionizationms / ms , esi - ms hs )分别获得差异蛋白点的肽指纹图谱( peptidemassoffingerprint , pmf )和部分序列信息，通过互联网上的expasy服务器中和ncbi的相关软件将这些信息和swissprot数据库进行匹配鉴定蛋白质种类。
Phopholipase c - 1 ( plc - 1 ) is widely known to play an important role in regulating cell proliferation and differentiation , development of the organisms , cell transformation and oxidative stress . till now , the mechanism how phopholipase c - 1 acts can not be thoroughly illustrated , nor has the interaction between plc - 1 pathway and other signal pathways been systematically reported . this research chose 2 - de + ms as the basic method from all kinds of proteomics strategies and compared the total protein expression map of mef genetically deficient in plc - 1 ( plc - 1 - / - ) to that of wild type mef ( plc - l + / + ) aimed to find some protein spots differentially expressed , thus we can discuss the impact of knockout of plc - 1 on signal transduction initiated by growth factors such as egf comprehensively . in this way , we can study the biological function of plc - 1 and mechanism of plc - 1 pathway indirectly , which will contribute a lot to further analysis 鉴于plc - 1发挥上述作用的机制尚未完全阐明， plc - 1通路与其他信号通路间的交联和代偿尚无系统报道，又因为以往的研究方法不够全面，本研究以野生型小鼠胚胎成纤维细胞( plc - 1 ~ ( + / + ) )和缺失磷脂酶c - 1的小鼠胚胎成纤维细胞( plc - 1 ~ ( - / - ) )为研究模型，在众多蛋白组学策略中选择了双向电泳+质谱( 2 - de + ms )作为研究手段，通过对比表皮生长因子( egf )刺激24小时后上述两种细胞的总蛋白质表达差异，全面地探讨敲除plc - 1对生长因子诱导的信号传递的影响，从而间接研究plc - 1生物学作用、信号传递机制及其代偿情况，为后续的深入研究打下基础。
