2 . cloning and sequence analysis of mod gene using the genomic dna and pistil total rna isolated from b . olereacea l . and b . napus l . as templates in pcr and rt - pcr reaction , the mod dna and cdna were amplified , recovered , cloned into vectors and sequenced Mod基因的克隆与序列分析以甘蓝和油菜的基因组dna和柱头总rna为模板，采用pcr和rt - pcr技术扩增moddna和cdna ，电泳后对目的带进行回收、秃隆、两端验证，测序获得其dna和cdna序列。
Two primers , designed according to the conserved regions of ban gene in arabidopsis thaliana , were used to amplify the ban homologous fragments from the genomic dna of brassica napus , b . chinensisl , b . juncea , a . thaliana and other cultural plants of cruciferae . the very similar pcr fragments were obtained from all the amplifications , which implicated that ban may be a conserved gene existing widely in the genomes of cruciferae . pcr fragments were cloned and confirmed by restriction enzyme digestion 参照拟南芥( arabidopsisthaliana ) ban基因与cdna设计引物，对甘蓝型、白菜型、芥菜型油菜，拟南芥及其它十字花科栽培品种的基因组dna进行pcr扩增，均扩增出与拟南芥ban基因扩增片段大小极其相似的dna片段，提示ban可能广泛存在于十字花科植物中。
Thl2 gene was finally amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 ) . the dna sequencing result shows that the genomic gene of thl2 is approximately 900 bp from brassica oleracea l . in contrast to that of 850 bp from brassica napus l . 采用阿r和rtpcr技术，从甘蓝和油菜基因组和柱头总则a中扩增获得了thlz基因，基因组中得到的基因大约为900hp和850hp ， cdna大约为477hp 。在thlz基因中首次发现了两个内含于，并且甘蓝和油菜thlz基因的内含于序列同源性低于ich ，并且大小也有差异。
The method of ctab was specifically improved for the extraction of genomic dna and total rnas from brassica oleracea l . and brassica napus l . 2 . arclboler gene was amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 ) 以甘蓝( 200110197 )和油菜( y578 - 2 )自交不亲和系的植株为材料，提取幼苗中的gdna及花蕾期的柱头、子房、花瓣、叶片、根的mrna ，并对mrna进行反转录合成cdna ，然后分别以gdna与cdna为模板扩增arc1蛋白编码序列。
The objectives of this study are to clone the ban fragment of brassica napus and to apply the floral - dip in the transformation of oilseed rape to establish a convenient method for oilseed rape breeding . ban is dfr - like gene in chalcone synthesis pathway functioned as a negative regulator , and has been well studied in arabidopsis thaliana 为利用转基因技术创造甘蓝型黄籽油菜，本文克隆了与甘蓝型油菜( brassicanapus )色素生物合成有关的ban基因同源片段，并探索了甘蓝型油菜花序浸泡法转基因新方法。
" xiyuan4 " , a b . olereacea l . and " 2001817 " , a b . napus l . were bred by southwest agricultural university . here using them as materials , we studied and explored thoroughly the dna and cdna sequences of thl2 gene and mod gene with bioinformatical methods . for the first time , based on sequence data , we analyzed the structure , heredity and variation of the dna sequences encoding thl2 and mod , and foresaw the molecular mechanism in self - incompatibility signal process 本研究以西南农业大学繁育的高度自交不亲和的‘西园4号’甘蓝和‘ 2001817 ’油菜为试材，采用生物信息学的方法全面研究和探索性的分析了thl2基因和mod基因的dna与cdna序列，首次从序列数据出发研究这两个编码基因的结构与遗传变异，以及在自交不亲和性信号转导中的作用机理
