Thl1 gene was also amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 ) 采用pcr和rt - pcr技术，从甘蓝和油菜基因组dna和柱头总rna中扩增获得了719bp的thl1基因和430bp的cdna序列。
In order to avoid promoter - homology - mediated gene silencing , we isolated another seed - specific promoter nap300 from brassica napus h165 to replace 75 promoter in constructed expression cassettes 将nap300与gus基因相连进行功能鉴定。荧光检测和组织化学染色的结果都证明此仅300bp的dna序列足以调控基因进行种子特异性表达。
With 5 male sterile lines in brassica napus l . as materials , the development of buds and stamens and pistils of male sterile lines in b . napus l . were researched and maked a comparison with the control group 摘要以5种不同类型甘蓝型油菜雄性不育系为材料，以保持系作对照，对甘蓝型油菜雄性不育系的花蕾及雌雄蕊干物质变化动态进行了研究。
The cdna of thl2 from brassica napus l . is 447 bp . thl2 gene contains two introns with wide divergences between brassica oleracea l . and brassica napus l . . however , the deduced amino acid sequence of thl2 protein from brassi 5ug的总rna为模板，进行定量rt pcr ，结果分析表明am基因在甘蓝中的表达特异性和油菜一样，均是在花柱柱头表达的。
3 . the mechanism of dna integration mediated by homologous recombination in chloroplast transformation was tentatively explored . we isolated two chloroplast dna fragments from brassica napus and used them as targeting sequence to integr 成功地通过h次转化获得整合并表达多基因的转基因烟草，缩短了研究周期，对相关转基因植物的研究有一定参考价值。
The results indicate that yellow seed variety of b . napus rape is as a mixture of various yellow seeds , there are significant differences among the lines tested and different segregation of individual plants each other in its penetrance of the seedcoat 研究了甘蓝型黄籽油菜不同品系材料、不同单株间千粒黄籽外显率及其与含油量、黄籽级数和千粒重的关系。
Abstract : the results indicate that yellow seed variety of b . napus rape is as a mixture of various yellow seeds , there are significant differences among the lines tested and different segregation of individual plants each other in its penetrance of the seedcoat 文摘:研究了甘蓝型黄籽油菜不同品系材料、不同单株间千粒黄籽外显率及其与含油量、黄籽级数和千粒重的关系。
Cloning and sequence analysis of thl2 gene using the genomic dna and stigma total rna isolated from b . olereacea l . and b . napus l . as templates in pcr and rt - pcr reaction , the thl2 dna and cdna were amplified , recovered , cloned into vectors and sequenced Thl2基因的克隆与序列分析提取甘蓝和油菜的基因组dna和柱头总rna ，采用pcr和rt - pcr技术扩增thl2dna和cdna ，对目的带进行回收，克隆并测序。
Homologous analysis in amino acid sequence showed that between thl2 in b . olereacea l . and trx4 in arabidopsis thaliana were 97 % in identity , a little difference to that of 86 % in identity between thl2 in b . napus l . and trx4 in a . thaliana 氨基酸水平上的同源性分析表明甘蓝thl2与油菜thl2和拟南芥中硫氧还蛋白trx4同源性较高。分别达到97和86 ，进化分析也表明thl2和trx4具有较高的亲缘关系。
The start codon is at 84 - 86bp , the stop codon at 1658 - 1661bp . the homologous comparison of the deduced amino acid sequence with other plant epsp synthase proteins shows the identity to those of b . napus , a . thaliana , tomato , n . labacum respectively are 92 % , 91 % , 80 % , 65 % 同源比较该段序列所编码的氨基酸序列可以发现epsp合成酶有较高的保守性，它与欧洲油菜、拟南芥、西红柿、烟草的同源性分别为920 、 91o 、 80o 、 65o 。
