转染试剂的英文
发音:
"转染试剂"怎么读用"转染试剂"造句
英文翻译手机版
- transfection reagent
- "转"英文翻译 revolve; rotate; turn; swive ...
- "染"英文翻译 dye
- "试剂"英文翻译 reagent; agentia; analoids
- "转染试剂对照" 英文翻译 : mock transfection
- "转染" 英文翻译 : transfection
- "rna转染" 英文翻译 : rna transfection
- "电转染" 英文翻译 : electrotransfection; electrotransfer
- "共转染" 英文翻译 : cotransfection
- "脂转染" 英文翻译 : lipofection
- "转染瘤" 英文翻译 : transfectoma
- "转染率" 英文翻译 : transfection efficiency
- "转染子" 英文翻译 : transfectant; transpeptidase
- "试剂" 英文翻译 : [化学] reagent; agentia; analoids◇试剂厂 chemical reagent works
- "拔染试验" 英文翻译 : discharge test
- "感染试验" 英文翻译 : infective stage larva
- "污染试验" 英文翻译 : test for contamination
- "压染试验" 英文翻译 : pressure dye test
- "基因转染" 英文翻译 : gene transfection
- "磷酸钙转染" 英文翻译 : calcium phosphate transfection
- "瞬时转染" 英文翻译 : transient transfection
- "稳定转染" 英文翻译 : stable transfection
- "细胞转染" 英文翻译 : cell transfection
- "细胞转染剂" 英文翻译 : cytofectin
- "脂(质)转染(法)" 英文翻译 : lipofection
- "转染实验" 英文翻译 : transfection experiment
例句与用法
- 2 . construction of chimeric mtb8 . 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8 . 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8 . 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments , respectively
3 .重组质粒在真核细胞中的表达: pm 、 pms 、 pmi和pmsl重组质粒用lipofectaminatmzo0o脂质体转染试剂转染cos一7细胞,进行瞬时表达, 48小时后,用rl ’ - pcr检测目的基因在mrna水平的表达;用westemblotting检测hil一12在蛋白质水平的表达。 - In order to study the function of cycling2 in vitro culturing cell line , we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent , and studied the function of cycling2 expression on the cell proliferation in vitro , further investigated the regulation mechanism of cycling2 . at the same time , we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue . material and method 1 material : piresneo vector was purchased from clonetech , plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640 , , dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit , mouse monoclonal antibody p21wafl ( in use ) , dab staining kit were purchased from maixin company
实验材料与方法1 .实验试剂高糖dmem 、 rpmll640和胎牛血清购自美国g山eo / brl公司; dmewf12 ( 1 : 1 )混合培养液购自美国hyclone公司;胰蛋白酶购自美国si目叮a公司; hepes由美国amersco公司分装;脂质体转染试剂( upofectalnineplusreageni )和以18为美国玩vitrogen公司产品; piresneo载体购自美国cloneteeh公司;质粒提取及纯化试剂盒购自德国qiagen公司; ultresensitive翎s一p免疫组织化学试剂盒;鼠单克隆抗体户3 ( do一7 )蛋白(即用型) ;鼠单克隆抗体p21waf , (即用型) ; dab染色试剂盒均购自福建迈新公司;鼠单克隆抗体pziwa曰(浓缩型) ;辣根过氧化酶标记羊抗鼠二抗购自北京中山公司; ecl试剂盒购自美国santacruze公司; dcproteinassay试剂盒购自bi 。
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