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识别位点的英文

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"识别位点"怎么读用"识别位点"造句

英文翻译手机手机版

  • recognition site

例句与用法

  • A further family of fibre optic chemical sensors has either surface atached molecular recognition sites or a reaction chamber for achieving specific molecular detection
    光纤化学传感器还具有表面分子识别位点或化学反应部位,可用于特定分子的检测。
  • The cbd tag of the fusion protein was cut by site - specific protease enterokinase at starting met of the target protein lt 27 . it was released from the cbd fusion tag efficiently
    利用融合蛋白中目的蛋白lt 27与cbdtag接头处的肠激酶特异性识别位点,用肠激酶处理粗纯化的融合蛋白,可将卜
  • A novel calix [ 4 ] arene - based fluorescent chemosensor bearing 2 - aminopyridine moiety and naphthalenic fluorphore was synthesized , and the chemical structure of the product was identified by ft - ir , ms - fab , nmr and elemental analyses
    摘要以杯[ 4 ]芳烃为平台、氨基吡啶基团为识别位点、蔡环为荧光基团,合成了一类新型荧光化学传感器。
  • After electrophorised on 1 % agarose gel , the pcr production was purified with agarose gel dna extraction kit . the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a . a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones . positive clone was identified by three ways : endonuclease digestion , pcr and sequencing . the result showed that the cloned sequence coincides with the designed sequence . this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit . the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ) . after 12 - 16 hours of culture , several colnes appeared on the plate . some positive clones were selected to extract their plasmid . these plasmids were digested by nco i and xho i and indentified by pcr . a contraction , mstnd - pet28a was generated . the result showed that the cloned sequence coincides with the designed sequence
    F _ 1长38bp , r _ 1长36bp ,其它片段均40bp长, f _ 1和r _ 1片段两端分别加上限制性内切酶nco和xho的识别位点序列。用成对单链片段进行延伸反应,然后用其他单链片段作为引物,进行pcr扩增,用dna快速纯化回收试剂盒回收所得254bppcr产物,与pmd18 - t载体连接、转化dh _ 5 。受体菌感受态细胞,利用蓝白斑遗传学筛选法筛选阳性克隆,提取其质粒,采用nco和xho双酶切鉴定,获得了254bp的片段;用pmd18 - t载体上的特异引物rv - m和m13 - 47进行pcr鉴定,获得300bp的片段。
  • Total rna was extracted from hepatic cells of mouse . a ecori and bamhi restriction sites were introduced into endostatin gene at specific primer f r , and endostatin was amplified by rt - pcr , this endostatin gene contained bamhi and ecori restriction sites at its 5 " and 3 " ends respectively
    从小鼠肝脏细胞中提取总rna 。设计合成一对特异引物,分别带有ecori和bamhi的限制性内切酶的识别位点。用rt - pcr法扩增endostatin的基因片段,在endostatin基因的两侧引入ecori和bamhi酶切位点。
  • It is divided to extracellular and intracellular part by transmembrane domain . there are 13 n - glycosylation sites , 20 protein kinase c phosphorylation sites , 28 casein kinase ii phosphorylation sites , 4 tyrosine kinase phosphorylation sites and 15 n - myristoylation sites in the extracellular part of bt - r3 protein . an integrin recognition sequences rod lies in intracellular part of bt - r3 protein
    跨膜区域( tmd )将它分为胞内和胞外两个部分,它的胞外有13个潜在的糖基化位点, 20个蛋白激酶c的磷酸化位点, 28个酪蛋白激酶的磷酸化位点, 4个酪氨酸酶的磷酸化位点, 15个豆蔻(十四烷基)酰化位点;它的胞内有1个整合蛋白( integrin )识别位点
  • Telomerase is a ribonucleoprotein complex ( rnp ) composed by its rna component and protein subunits . telomerase can synthesize telomeric dna onto chromosomal ends using its own rna component as a template , elongate the length of telomere , increase cell life and even induce cell immortalization
    端粒酶( telomerase )是由端粒酶rna和蛋白质组成的一种核糖核蛋白复合物( rnp ) 。端粒酶含有引物特异识别位点,能以自身rna为模板,逆转录合成端粒dna并加到染色体末端,使端粒延长,从而延长细胞的寿命甚至使其永生化。
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