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离子交换层析的英文

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"离子交换层析"怎么读用"离子交换层析"造句

英文翻译手机手机版

  • ion exchange chromatography
  • ion-exchange chromatography
  • ion-exchange column chromatography

例句与用法

  • Polyethylene glycol - accompanied ion - exchange chromatography to purify recombinant hepatitis b virus surface antigen
    聚乙二醇伴随式离子交换层析分离重组乙肝病毒表面抗原
  • An active metabolite was obtained by purification with precipitated by ethanol , sephadex g - 25 gel , deae - cellulose ion exchange resin and silica gel column chromatography
    经乙醇( 95 )沉淀、 sephadexg - 25凝胶层析、 deae -纤维素离子交换层析和硅胶层析纯化,得到抑制黑曲霉生长的单一组分。
  • But the whole level of serum titer in combined vaccine group was higher than others . igg was extracted by salting out with ammonium sulfate and purified by ion exchange chromatography with deae cellulose
    对分离到的血清用饱和硫酸铵盐析法提取igg ,并用deae纤维素离子交换层析法对提取的igg进行纯化。
  • Then bacteria were collected by centrifugation and split by ultrasonic method . the purified products were analyzed by tris - tricine sds - page . the immuno - activity of the recombinant proteins were analyzed by western blot
    将离心收集到的菌体超声破壁,离心分离,收集上清液,进行离子交换层析和分子筛分离纯化。
  • The ps ii native fractions ( 20 % and 30 % ) were loaded onto a deae column . the fraction eluted with 150 mm nacl was presented dcip reduction activity and was highly depleted in chi c and xanthophylls , and as such could be considered a ps ii core complex
    对于有dcip光还原活性的20和30层带的复合物,进一步deae离子交换层析纯化。 150mmnacl洗脱纯化后的样品经过荧光激发光谱测定发现,已经去除了叶绿素c和墨角藻黄素,并且仍然具有dcip的光活性,分析是ps核心复合物。
  • The enzyme activity in fermentation liquid could be inhibited by pmsf and dfp . the fermentation liquor also showed good dehairing activity . the alkaline protease ( named dhap , dehairing alkaline protease ) in the fermentation liquid was purified with hydrophobic interaction chromatography , ion exchange and gel filtration
    通过cm - sepharosefastflow离子交换层析, deae - sepharosefastflow离子交换层析, sephacryls - 100 , sephacryls - 200凝胶过滤层析,疏水层析等纯化步骤对短小芽孢杆菌发酵液中的碱性蛋白酶进行了纯化。
  • The purified enzyme had a specific activity of 68 . 6 u / mg protein . overproduction of pga was often limited by translocation and / or periplasmic processing steps , subsequently resulted in intracellular accumulation of various types of pga precursors and then formed inclusion bodies in the cytoplasm and / or periplasm
    经deae - sepharosecl6b离子交换层析和butyl - sepharosecl4b疏水层析,即可得纯度提高20倍、比活为68 . 6u mg的青霉素g酰化酶,两步纯化的总得率达91 。
  • Strain bl21 , and gene expression was induced by iptg . the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene . using ion exchange chromatography and gel filtration chromatography , the chimera proteins were purified , and both of them showed a single band in tricine - sds - page . the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities , but gain platelet aggregation inhibitory activities as well
    通过离子交换层析和凝胶过滤层分别对两种嵌合体蛋白进行纯化,纯化产物在tricine - sds - page中都显示为单一条带。活性分析结果表明两种嵌合体蛋白在保留水蛭素抗凝血酶活力的同时,还呈现抗血小板聚集活性。
  • Under these conditions the fibrinolytic activity of the supernatant can reach 820 urokinase units per milliliter broth . ba - dfe was purified from the supernatant of b . amyloiquefaciens dc - 4 culture broth by ammonium sulfate precipitation , ion - exchange chromatography on cm - and deae - sepharose fast flow , hydrophobic interaction chromatography on phenyl sepharose 6 fast flow and gel filtration on sephadex g - 50 . the purified enzyme displayed thermophilic , hydrophilic and strong fibrinolytic activity
    通过硫酸铵分级沉淀、 cm - sepharosefastflow和deae - sepharosefastflow离子交换层析、 phenylsepharose6fastflow疏水层析和sephadexg - 50凝胶过滤等方法,从解淀粉芽孢杆菌dc - 4的发酵液中分离纯化出电泳纯的ba - dfe 。
  • Purification and characterization of phytase from a . niger an 01001 a . niger an01001 was inoculated on solid media and cultivated at 30 for 5 days . proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5 . 5 ) . the molecule weight of the phytase protein was determined as about 78kd by sds - page . the purification procedures include ammonium sulfate precipitation , deae - cellulose ion - exchange chromatography , gel electrophoresis and electroelution
    3 .植酸酶的分离纯化及其性质研究黑曲霉ano1001经固体发酵,用缓冲液抽提后,经硫酸按沉淀, deae一纤维素离子交换层析,聚丙烯酞胺凝胶电泳和电洗脱等纯化步骤获得的植酸酶,用sds一page检测为一条均一谱带,其分子量约为78kd 。
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