氨苄青霉素抗性的英文

• amicillin resistance

• "氨"英文翻译    ammonia; hydrogen nitride
• "苄"英文翻译    Glutinous rehmannia
• "青霉素"英文翻译    penicillin; penethamate
• "抗性"英文翻译    xìng] resistance
• "氨苄青霉素" 英文翻译 :    aminobenzyl penicillin; ampicillin sodium; ampicillinum; ampicilln; penbritin
• "青霉素抗性" 英文翻译 :    penicillin resistance
• "氨苄青霉素钠" 英文翻译 :    ampicillin sodium; sodium ampicillin
• "氯氨苄青霉素" 英文翻译 :    cefaclor
• "匹氨苄青霉素" 英文翻译 :    pivampiciilin
• "酞氨苄青霉素" 英文翻译 :    talampicillin
• "氧氨苄青霉素" 英文翻译 :    amoxicillin
• "哌氨苄青霉素" 英文翻译 :    avocin orocin pipril
• "羟氨苄青霉素" 英文翻译 :    amoxicillinum; amoxil; amoxycillin
• "氨苄西林,氨苄青霉素" 英文翻译 :    damp; sodium ampicillin
• "p-羟氨苄青霉素" 英文翻译 :    p-hydroxyampicillin
• "氨苄青霉素(阿比西林)" 英文翻译 :    ampicillin
• "氨苄青霉素干糖浆" 英文翻译 :    ampilin dry syrup
• "氨苄青霉素胶丸" 英文翻译 :    ampicillin capsule
• "氨苄青霉素敏感的" 英文翻译 :    ampicillin-sensitive
• "氨苄青霉素酞酯" 英文翻译 :    tapc
• "口服氨苄青霉素" 英文翻译 :    ampicillin for oral suspension
• "耐氨苄青霉素的" 英文翻译 :    ampicillin-resistant
• "匹夫氨苄青霉素" 英文翻译 :    pivaloyloxymethyl ampicillinate; pivaloyloxymethyl d-a-aminobenzylpe-nidlli-hate
• "匹呋氨苄青霉素" 英文翻译 :    pvpc
• "三水氨苄青霉素" 英文翻译 :    pen a

• In this paper , first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv . the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector . after transforming e . coli dh5 a , ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr . presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc . comparing the aquired sequence of 3abc with that of reference strains , the homology is more than 99 percent . the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo . lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene , which happened to form a terminator codon behind 3ab gene , but it contained the complete open reading frame ( orf ) of 3ab gene . positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ) , bacteria were detected by sds - page and western blotting after properly treated . the results showed that the 3ab gene expressed successfully in e . coli and 33 . 5ku fusion protein can be recognized by the positive bovine serum of fmdv . the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
  扩增产物连接到pgem - teasy载体中，转化大肠杆菌dh5菌株，筛选氨苄青霉素抗性菌落，提取质粒经酶切鉴定、 pcr分析以及确证性测序证明，所克隆的1500bp左右的片段含有完整的3abc基因，与国外参考序列相比，同源性在99以上。将重组质粒pgem - 3abc和表达载体ptriex - 4neo分别用sal和bgl与xho和bgl消化后，亚克隆3abc基因至原核表达载体ptriex - 4neo中，通过酶切鉴定、 pcr扩增以及序列分析，发现克隆到ptriex - 4neo载体上的片段于3abc基因708bp处出现了17bp的缺失，碰巧在3ab基因后形成一终止密码子，但3ab基因的阅读框架完整，选出含有3ab基因完整阅读框架的阳性克隆，用iptg诱导表达，收集菌液进行sds - page电泳、 westernblotting分析，结果表明， 3ab基因在大肠杆菌中成功表达，其表达产物为分子量33 . 5ku的融合蛋白，并能被口蹄疫病毒阳性血清识别。经薄层扫描分析，表达量占总蛋白量的26以上。