氨基酸组成分析的英文
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"氨基酸组成分析"怎么读用"氨基酸组成分析"造句
英文翻译手机版
- amino acid composition analysis
- "氨基酸"英文翻译 amino acid; gamma-aminobutyr ...
- "组成"英文翻译 form; make up; compose; form ...
- "分析"英文翻译 analyse
- "氨基酸组成分析仪" 英文翻译 : amino acid analyzer; amino acid composition analyzer
- "氨基酸组成成分" 英文翻译 : amino acid composition
- "氨基酸组成" 英文翻译 : amino acid composition
- "种子氨基酸组成" 英文翻译 : seed amino acid composition
- "主要氨基酸组成" 英文翻译 : the essential amino acid composition
- "由12种氨基酸组成的溶液" 英文翻译 : sohamin g
- "氨基酸成分分析仪" 英文翻译 : amino acid composition analyzer
- "烃组成分析" 英文翻译 : aromatic hydrocarbons analysis
- "组成分析" 英文翻译 : component analysis; compositional analysis
- "氨基酸分析" 英文翻译 : amino acid analysis
- "组成分析,成分分析" 英文翻译 : composition analysis; compositional analysis
- "颗粒组成分析" 英文翻译 : grain-size analysis
- "矿物组成分析" 英文翻译 : mineralogical analysis
- "组成成分分析" 英文翻译 : component analysis
- "氨基酸分析器" 英文翻译 : aa; amino acid analyser; amino acid analyzer
- "氨基酸分析仪" 英文翻译 : amino acid analyzer; amino-acid analyzer; amino-acid andlyser
- "烃类族组成分析" 英文翻译 : hydrocarbon grouanalysis
- "氨基酸序列分析" 英文翻译 : amino acid sequence analysis
- "氨基酸序列分析器" 英文翻译 : amino acid sequence analyzer
- "氨基酸自动分析仪" 英文翻译 : amino acid autoanalyzer; automatic amino acid analyzer; automatic amino-acid analyzer; automatic amion-acid analyzer
- "荧光氨基酸分析仪" 英文翻译 : fluorescence amino acid analyzer
- "自动氨基酸分析仪" 英文翻译 : automatic amino acid analyser
例句与用法
- It ' s pi is 5 . 2 as determined with ief . amino acid composition analysis showed that one subunit of 6 - phosphogluconate dehydrogenase has about 480 amino acids and there are plentiful of ala , asp , leu , ser , glu , thr , phe , lys in it
氨基酸组成分析表明:枯草芽孢杆菌6 -磷酸葡萄糖酸脱氢酶的亚基由约480个氨基酸残基组成,富含丙氨酸、门冬氨酸、亮氨酸、丝氨酸、谷氨酸、苏氨酸、苯丙氨酸、赖氨酸。 - The hwtx - i gene was chemically synthesized according to its known cdna sequence , the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor , the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis , then it was transformed into host strain gs115 , a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations , the multicopy transformant was named gh1 . gh1 was cultivated in flasks . after 6 days of induction by 0 . 5 % methanol , the supernatant was checked by 16 . 5 % tricine - sds page , which showed there was a band in the position of 3 . 5 - 6 . 1kd , then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column , after reverse phase hplc of ci8 and vacuum drying , the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page , maldi - tof mass spectrometry , amino acid composition analysis , the n - terminal amino acid sequence and its biological activity , the final field of the purified rhwtx - i was about 80mg / l , accounting for 23 . 6 % of it total secretory proteins
将带有hwtx -基因的ppic9k经blgii线性化后,转化酵母宿主菌gs115原生质体后经筛选阳性克隆并经表型鉴定为his ~ + mut ~ s酵母菌,进一步用遗传毒素g418筛选多拷贝的转化菌株,命名为gh1 ;将gh1甲醇酵母菌用0 . 5的甲醇诱导表达,发酵上清经90饱和度的( nh _ 4 ) _ 2so _ 4沉淀, yw - 3 ( mwc03000 )的超滤膜超滤,再经cm阳离子交换, c _ ( 18 )反相hplc纯化得到分子量为4kd左右的组分,其中4289 . 05的组分经质谱鉴定,氨基酸组成分析和序列测定为正确的表达产物,生物学活性表明其活性为天然毒素活性70 % ,表达量为80mg / l 。
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