多拷贝的英文
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"多拷贝"怎么读用"多拷贝"造句
英文翻译手机版
- mrna multicopy
- multicopy
- "多"英文翻译 many; much; more
- "拷贝"英文翻译 copy; replica
- "多拷贝平均" 英文翻译 : multiple copy averaging
- "多拷贝原粒" 英文翻译 : multicopy plasmid
- "多拷贝质粒" 英文翻译 : multicopy plasmids
- "多拷贝平均法" 英文翻译 : multiple copy averaging
- "拷贝" 英文翻译 : [电影] copy; replica◇拷贝机 letterpress; 拷贝片 print; 拷贝纸 copy paper; copying paper
- "单拷贝" 英文翻译 : single copy
- "防拷贝" 英文翻译 : copy prevention
- "拷贝,副本" 英文翻译 : duplicate, copy
- "拷贝,复制" 英文翻译 : copy
- "拷贝板" 英文翻译 : copy board
- "拷贝机" 英文翻译 : copyall; copying a aratus; copying apparatus; steand repeat machine
- "拷贝卷" 英文翻译 : copy volume
- "拷贝猫" 英文翻译 : copycat
- "拷贝片" 英文翻译 : print
- "拷贝器" 英文翻译 : copier
- "拷贝室" 英文翻译 : copy room
- "拷贝数" 英文翻译 : low copy number; numberofcopies
- "拷贝台" 英文翻译 : copyboard
- "拷贝王" 英文翻译 : kingcopy
- "拷贝纸" 英文翻译 : copy paper; copying paper; copying ti ue; copying tissue; second sheets; separstor; tissue paper
- "块拷贝" 英文翻译 : block copy
- "零拷贝" 英文翻译 : zero-copy
- "盲拷贝" 英文翻译 : blind carbon copy
例句与用法
- Gene duplication is defined as segment of dna duplicate one or more copies , it is believed to be a major mechanism for the generation of evolutionary novelty
摘要基因倍增指基因组中含有基因的dna片段复制出一个或更多拷贝的过程,是进化出新物种的主要原因。 - The whole gene was added every six hours instead of all in one time . in proper time , they were mixed and continued to ligate to multi - copy in the same direction
通过控制基因和接头加入的量及次序,可得到两侧有ecor和sal酶切位点的同向串连的多拷贝基因。 - Multicopy clones were screened from vary levels of g418 - ypd plate and cultured and induced with methanol to secrete interesting peptide , which was identified by sds - page and western blotting
培养多拷贝插入的酵母细胞,用甲醇诱导目的蛋白的分泌, sds - page和westernblotting鉴定表达蛋白。 - After linerization , the recombinant plasmid was transformed into pic hia pastoris by electroporation , which then were cultured in md plate free of histidine , from which the positive colones were propagated
重组质粒线性化后,用电击法将重组质粒转化入巴氏毕赤酵母,在缺组氨酸的md板上筛选阳性菌落,然后用不同浓度的g418 ? ypd板筛选多拷贝插入单菌落。 - 2 . southern blotting analysis indicated that sod2 gene had been integrated into all of the transgenic plants ' genomes . the copy number of sod2 in transgenic plants was mostly 1 to 5 by southern analysis
Southern结果表明,野生型植株(对照)没有明显的杂交信号,转基因株系有强阳性信号,转基因植株中插入外源基因的拷贝数不同,既有单拷贝的,又有两个拷贝及多拷贝的,拷贝数从1 - 5不等。 - Based on the blast analysis and other studies , osddl mutant was a multi - copy - insertion mutant , and one of the insertion sites was in an nptii like transposase gene , whereas osdd2 , a single - copy - insertion mutant was disrupted at a gene encoded wrky transcript factor
Osdd1突变体可能是多拷贝插入,其中一个插入到nptii转座酶类似基因。 osdd2突变体为单拷贝、插入到一个wrky类转录因子基因5翻译起始区附近。 - Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid , and induced with methanol to secrete interesting peptide . the supernate of pichia pastoris culture was analysed by sds - page and western blotting . a reactive band , which the apparent molecular weight is 36kd , can be detected with sheep anti - hcmv polyclonal antibodies
重组质粒转化巴氏毕赤酵母, g418筛选出多拷贝插入的单克隆,甲醇诱导多拷贝插入的单克隆酵母细胞分泌目的蛋白,培养液上清经sds - page电泳分析,在蛋白质印迹中检测到培养液上清有一表观分子量为36kd ,能与羊抗hcmv多克隆抗体发生发应的条带。 - Multi - copies insertion transformants were screened on g418 plates . the recombinant protein was proved to have biological activity of hydrolyzing n - carbamoylphenylalanine into phenylalanine through enzyme activity assay . the n - carbamoylase activity of recombinant was 2 . 26 and 2 . 15 times higher than that of arthrobacter bt801 and dh5a / puc18 - 169
将hyucdna片段连接到真核表达载体ppic3 . 5k上,经bgl酶切线性化,通过peg法转化导入毕赤酵母gs115感受态细胞,利用g418抗性筛选得到12个插入多拷贝目的基因的转化子。 - Methods : 1 . expression and purification of recombinant human ctla4 extracellular domain : a 400bp dna fragment of ctla4 extracellular domain was obtained from the pctla4 / ig plasmid with genetical technique . then , this dna fragment was inserted into ppic9 plasmid to construct the single copy plasmid of yeast expression system ( ppic9 - ctla4125 ) . furthermore , the multi - copy plasmid ( ppic9k - ctla4125 ) was constructed
重组人ctla4胞外区蛋白在酵母gs115中的表达和纯化:首先通过基因操作技术,从pctla4八g质粒中扩增400hp的ctla4胞外区片段;将ctla4胞外区片段插人ppicg质粒中获取单拷贝酵母表达质粒ppicg ctla4125并进一步构建多拷贝酵母表达质粒ppicgk ( 。
- 更多例句: 1 2
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