zeocin中文是什么意思
发音:
用"zeocin"造句"zeocin"怎么读"zeocin" in a sentence
中文翻译手机版
- [网络] 博莱霉素;博来霉素;抗菌素
- "zeodur" 中文翻译 : n. 硅酸盐阳离子交换剂
- "zeo-karb 315" 中文翻译 : 315磺酚阳离子交换树脂
- "zeokarb" 中文翻译 : n.阳离子交换树脂
- "zeo-karb 226" 中文翻译 : 226羧酸阳离子交换树脂
- "zeokarb h" 中文翻译 : 离子交换树脂(磺化碳)
- "zeo-karb 225" 中文翻译 : [树脂] 磺酚阳离子交换树脂
- "zeolex 23" 中文翻译 : n.硅酸铝钠
- "zeo-karb 216" 中文翻译 : 216羧酸阳离子交换树脂
- "zeolex 80" 中文翻译 : n.硅酸铝钠
- "zeo-karb 215" 中文翻译 : 215磺酚阳离子交换树脂
例句与用法
- After dna sequencing was correct, it was transformed into p . pastoris km71h strain by electroporation . highly resistant recombinant was isolated by increasing concentrations of zeocin ? positive clone was selected by pcr . the positive clone was induced with methanol
抗生素zeocin~(tm)浓度梯度筛选高拷贝菌株,分别提取这些高拷贝菌株的基因组dna,以此为模板做pcr反应鉴定阳性克隆。 - The positive transformants with the integrates mn-sod gene and cuzn-sod gene were identified by zeocin-resistance, pcr screening and expression in p . pastoris . the recombinant mn-sod protein and cuzn-sod protein was successfully expressed in pichia pastoris based on the evidences that the obvious activity of sod existed in native-page and enzymatic activity test
pcr鉴定进一步说明,目标基因已经重组到宿主基因组染色体上;0.5甲醇诱导表达后,活性电泳出现明显活性条带,重组酵母发酵液中mn-sod的活性约是对照菌株sod活性的2.6倍;cuzn-sod酶活力约是对照菌株sod活性的1.3倍。 - The positive transformants with the integrates mn-sod gene was identified by zeocin-resistance, pcr screening and expression in p . pastoris . the recombinant mn-sod protein was successfully expressed in pichia pastoris based on the evidences that a relative molecular weight about 23kd appeard in sds-page, the obvious activity of sod existed in native-page and enzymatic activity test, and mn-sod activity was specific base on the inhibition with the mixture of chloroform-enthanol ( 3 : 5 / v : v ) and potassium cyanide . two secreted plasmids ppiczaa-sodm18 and ppiczaa-sodc were constructed and after there linearization were transferred into chromosome of pichia pastoris gs115 by electroporation
pcr鉴定及mut表型分析进一步说明,目标基因已经重组到宿主菌基因组染色体上;0.5甲醇诱导表达后,sds-page结果显示,表达的蛋白相对分子量约为23kd,活性电泳出现明显活性条带;酶活性测定显示,重组菌株sod活性比对照提高5倍左右;氯仿-乙醇(3:5v:v)和kcn(5mmoll)抑制反应进一步证明,所表达的sod为锰超氧化物歧化酶。
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