x-gal造句

• Then the linked products were transformed into the high competent cell of e . coli dh5a . based on - complementation of the detective - galactosidase , positive recombinant clone were screened from x - gal plate
  从引物和基因序列的比对分析结果看， zhyf006序列与上下游引物的配对比例分别为s4
• Fixed with gluteraldehyde , stained with ph 7 . 4 phosphate buffered x - gal dye solution , observed by reverse microscope , the results are as follow : the percent of larvae expressing lacz in midgut showed that fb - 28 improved the sensitivity of larvae to virus
  病毒感染后的8h ， 12h ， 16h ， 20h提取中肠组织，按常规石蜡包埋切片进行洗涤、透明、浸蜡、包埋、切片、粘片等操作程序，制成连续切片，在倒置显微镜下逐一进行观察计数。
• After obvious cytopathogenic effects developed , virus - contained supematants were harvested , and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal . single blue plaques were picked , and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification . the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain
  该载体与具有高度感染性的bartha - k61株基因组dna通过脂质体加plus法共转染vero细胞，采用甲基纤维素固定病变， x - gal染色，经过10代蓝斑纯化获得了一株稳定表达lacz基因的ge tk基因缺失突变株，命名为rprv - lacz 。
• A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a , yielding a 1 . 7kb band . the segment was linked to puc19 plasma dna by means of t4 dna ligase , transformed into e . coli jm109 permissive cells , and incubated on lb fray containg amp , x - gal and iptg . small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr , resulting in recombinant plasma puge dna containing prv ge
  用t _ 4dna连接酶使ge基因与经bamhi 、 kpni同样双酶切的puc19质粒dna连接；用连接产物转化大肠杆菌jml09感受态细胞，置含amp 、 x - gal和iptg的lb平板上培养12 20小时；挑取白色菌落于选择性培养基扩大培养，碱裂解法小量提取质粒dna ，并进行酶切分析鉴定，结果获得整合有prvge基因的重组质粒pugedna ，并与其它prv分离株进行ge基因序列同源性分析。
• Thus the objective of this study is to find some ways to improve the efficiency of cell therapy , that is . to optimize the microenvironment of nscs and in turn prompt them to functinally repair abnormal cns . in the first part , optimization of x - gal staining method , which is correlated with ph , incubating time , perfusion and some other parameters , was successfully got and used in the subsequent experiment , viz , comparing the behaviours of primary nscs and immortal nsc line after transplantation
  鉴于此，为寻求各种方法提高移植效率，改善外源nscs存活及分化，进而促进其实现功能性替代，为临床nscs治疗提供理论基础，本实验进行了以下三部分的研究：实验第一部分研究了以lacz作为移植细胞标志基因时x - gal染色的影响因素，并得出排除非特异背景的优化条件。