" when you have an r gene , you can have complete resistance , " says john mundy , a researcher at copenhagen university
- 33 - 3 . the blindness of homology - based candidate gene method for cloning resistance gene originated from diversity and unpredictability of resistance genes
Based on the guard hypothesis , tm - 22 and mip204 could be a guardee and a guardian cooperated in pr gene induction in resistance activity . 3
The results indicate that conserved motif glpl and cfly does not exist in ht gene , in other words , ht gene does not belong to the type of nbs - lrr resistance gene
All kind of oligonucleotides ( 16 sense primers , 8 antisenset primers , 128 different primer combinations in all ) were designed from the conserved motifs glpl and cfly ( distance between them is approximately 210bp ) which existed only in the type of nbs - lrr resistance gene and used as primers for scanning the genomic dna . no specific products ( which is approximately 210bp in length and obtained from genomic dna containing ht gene ) was obtained though amplified products of approximately 500 - looobp appeared in six different primer combinations
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