The pet30 a + prokatyotic expression vector with a insertion fragment of fad2 cdna coding sequence is constructed to express a fusion fad2 protein with a n - terminal s - tag . with this tag , the protein is easy to detect and purify
Construction and expression of tandem multi - copy gene edcoding ca ( 1 - 8 ) me ( 1 - 10 ) hybrid fused acidic peptide to improve antimicrobial peptide ' s expression , a novel mass - production method is proposed . it is based on the neutralization of the positive charges of antimicrobial peptide by fusing to an acidic peptide to avoid the lethal effect of the expressed peptide on the host cells
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