lactase造句

• Study of isolation and purification of lactase from aspergillus niger d
  226乳糖酶分离纯化研究
• Study of the relationship between urticaria diarrhea and lactase
  药物性腹泻病与乳糖酶的关系研究
• The influence of ca2 fe2 mg2 zn2 na and k on lactase activity in sucking piglets
  对哺乳仔猪乳糖酶活力的影响
• A similar , less severe , metabolic disorder is congenital lactase deficiency
  类似的，病症略轻为一点的代谢紊乱疾病是乳糖分解酶素缺乏症。
• Many asian adults are lactose intolerant , as a result of a lack of the digestive enzyme lactase in the gut
  许多亚洲成年人有乳糖不耐受症，因为他们消化道中缺乏消化乳糖的乳糖酶。
• The former was 0 . 475u / ml , and the later was 0 . 134u / ml . 2 . determination of the properties of lactase expressed in e . coli
  原核表达乳糖酶的酶学性质测定制备原核表达乳糖酶的原酶液。
• Some premature babies have a temporary form of lactose intolerance because their bodies are not yet producing lactase
  一些早产的宝宝会暂时的有乳糖不耐受症状是因为他们体内还未开始分泌乳糖分解酶。
• At present , most of lactases used in industry production come from kluyveromyces , aspergillus niger and aspergillus oryzae
  目前，工业生产中使用的乳糖酶主要来源于乳酸克鲁维斯酵母菌、黑曲霉和米曲霉。
• This disorder , apparent within 10 days of birth , occurs when brush - border lactase activity ( required for the digestion of lactose ) in the small intestine is low or absent at birth and is characterized by diarrhea and malabsorption
  此病是由于宝宝出生时小肠内的乳糖分解酶素活性很低或没有活性，病症通常在宝宝出生10天内显现：拉肚子以及吸收不良。
• Cloning and expression in e . coli of lactase . specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis . klac gene was amplified by pcr and subsequently cloned
  乳糖酶基因的克隆及原核表达以乳酸克鲁维斯酵母( kluyveromyceslactis )菌株k538的基因组dna为模板，设计引物，利用pcr获得乳糖酶基因klac ，经dna测序验证，得到克隆t1549 。
• Preparing the lactase solution expressed in e . coli , we determinated the optimum ph , ph stability , optimum reaction tempreture , thermal stability , effect of metal ions and chemical reagent , km and vmax its optimum ph was 5 . 8 and 6 . 4 to 7 . 5 , and the optimum tempreture was 45 to 52
  以onpg为底物，分别测定了其最适ph值及ph稳定性、最适温度及热稳定性、金属离子和化学试剂的影响以及k _ m值和v _ ( max )值。
• The lactase gene from kluyveromyces lactis was researched in the thesis . the cloned gene was expressed in e . coli and the properties of lactase was determinated . in addition , we studied the expression of lactase gene in the methylotrophic yeast pichia pastoris
  本论文从一株乳酸克鲁维斯酵母菌中克隆获得乳糖酶基因，在大肠杆菌中进行表达并测定其酶学性质，同时也对利用巴斯德毕赤酵母( pichiapastoris )系统表达该基因进行了探索。
• Klac gene was inserted into the vector ppic9 to construct recombinant plasmid p1c9154983 , and then pic9154983 was transformed by electricity pulse into gs115 , pichia pastoris acceptor . after screening , we obtained recombinant yeast 1 # , 8 # and 24 # , that is , klac gene was integrated into the genome dna of gs115 . by detecting the lactase activity , we knew that the klac gene was expressed in vivo of pichia pastoris , but the lactase was not secreted
  三个重组菌株经甲醇诱导培养未检测到分泌的乳糖酶，但胞内均检测到有活性的乳糖酶，分别为0 . 1392u / ml 、 0 . 3702u / ml和0 . 2214u / ml ，说明乳糖酶基因klac在巴斯德毕赤酵母中获得表达。
• So the lactase was suitable to whey at ph7 . 0 , but could n ' t react at high tempreture . the metals , fe2 + , mn2 * , ca2 + , mg2 + and zn2 + , can prominently improve lactase activity , but cu2 + would strongly inhibit the activity . according to the effect of edta , we concluded that the lactase depends on metal ions in a certain extent
  乳糖酶基因klac在巴斯德毕赤酵母中表达将klac基因插入到载体ppic9上构建重组表达质粒pic9154983 ，电击转化巴斯德毕赤酵母受体菌gs115 ，经筛选和pcr鉴定， 1 # 、 8 #和24 #为重组菌株， klac基因整合到gs115基因组中。
• Klac gene was ligated to the pet - 30a ( + ) vector for expression . then the recombinant plasmid petlac4 was transformed into e . coli bl21 . the positive transformants were induced at different temperature for three hours by iptg with the final concentration of lmm . sds - page analysis and lactase activity assay showed that klac gene was expressed in e . coli , and that the expression level at 28 was much higher than that at 37
  表达产物的sds - page分析和酶活性测定表明， klac基因在大肠杆菌中获得活性表达，其中低温条件28下的表达水平明显高于37 ， 28诱导的细胞经超声波破碎所测酶活力为0 . 475u / ml ， 37仅为0 . 134u / ml 。