epsp造句

• Epsp excitatory post - synaptic potential
  兴奋性突触后电位
• Epsp synthase is a attractive prime target for many antimicrobial agents and herbicides
  该酶是许多抗生素、除草剂作用的首选靶标酶。
• At present , nobody have reported the phylesis research through dna sequence of epsp
  目前用epsps基因编码区的dna序列来研究物种的进化关系尚未见报道。
• The epsp synthase is a necessary enzyme of the shikimate pathway and it is very conservative
  Epsp合成酶是莽草酸途径的一个关键酶，其成熟蛋白在进化中极为保守。
• 5 - enolpyruvlshimimate - 3 - phosphate synthase ( epsp synthase ) is a necessary enzyme of the shikimate pathway in prokaryote , yeasts , fungi , apicomplexan parasites , plants and algae
  原核生物、酵母、真菌、顶配位寄生虫、植物和藻类中的epsp合成酶是芥草酸途径中的一个氨基酸必需酶。
• Plants can obtain high doses glyphosate tolerance if the enzyme of this gene is over produced and accumulated or herbicide - insensitive enzymes produced due to some amino acid mutation in active sites
  植物中epsp合成酶的过量表达或某些活性位点氨基酸的突变对高剂量的草甘膦有较强的耐受性。
• The amino acid sequences of epsp synthase between high plants have very high identity , but the identity of transport peptide of epsp is as low as 23 % between plants
  高等植物成熟的epsp合成酶的氨基酸序列之间具有很高的同源性，但epsp合成酶的叶绿体运输肽的氨基酸序列同源性极差，各植物间仅23左右的同源性。
• The start codon is at 84 - 86bp , the stop codon at 1658 - 1661bp . the homologous comparison of the deduced amino acid sequence with other plant epsp synthase proteins shows the identity to those of b . napus , a . thaliana , tomato , n . labacum respectively are 92 % , 91 % , 80 % , 65 %
  同源比较该段序列所编码的氨基酸序列可以发现epsp合成酶有较高的保守性，它与欧洲油菜、拟南芥、西红柿、烟草的同源性分别为920 、 91o 、 80o 、 65o 。
• Two partially overlapping cdna fragments which were cloned from o . violaceus using the rt - pcr , 3 ' race ( rapid amplification of cdna ends ) and 5 ' race technique was assembled a 1758bp full cdna sequence of epsp synthase ( accession number in genbank : af440389 )
  采用rt pcr技术与3 ’ race和5 ’ race的结合，以诸葛菜的总rna为反转录的模板，克隆、拼结出了诸葛菜的epsp合成酶的全长。 dna序列（该段cdan的genbank的登录号为： af440389 ） 。
• The nj map and simplest principle map based on 3 ' end coding sequences are identical to the two maps based on the complete coding sequences . this shows that 3 " end coding sequences of epsp can replace the complete coding sequences to analyze the molecular evolution
  用epsps基因全长编码区的dna序列构建的nj图，最简约树与用其3 ’端编码区dna序列构建的树形图相同，表明完全可以用epsps基因3 ’端编码区dna序列代替基因全长编码序列作进化分析。
• The deduced amino acid sequence of the epsp synthase from psedomonas fluorescens g2 was compared to epsp synthase from various species . it shares 30 % homology with the epsp synthases from e . coli and salmonella typhimurium in class i and is different from patent - protected mutational sites
  将本实验获得的荧光假单胞菌g2epsp合成酶推导的氨基酸序列与class中大肠杆菌和鼠伤寒沙门氏菌来源的epsp合成酶同源性约为30 ，且不含有专利保护的突变位点。
• The predicted protein contained 441 amino acids . the activity of the epsp synthase encoded by these two subclones was shown by complementation of the aroa mutation in e . coli strain er2799 , indicating two subclones possess a intact activity of the enzyme 5 - enolpyuvyl - 3 - phosphoshikimic acid synthase
  采用互补试验验证分离的与草甘膦耐受相关的dna片段的生物学功能，结果显示r3h1和r7h1均能使大肠杆菌epsp缺陷型菌株er2799恢复在限制性培养基上的生长能力，证明这两个亚克隆的插入片段具有完整的epsp合成酶功能。
• It shares less than 25 % homology with the epsp synthases in class ii . it also shares 24 . 5 % homology with the epsp synthase isolated by monsanto company and does not contain any conservative sequences and mutational sites protected by patents . all of the above shows great potential for future development
  与class的epsp合成酶同源性低于25 ，其中与monsanto公司分离的根癌农杆菌( agrobacteriumtumefaciens ) cp4来源的epsp合成酶同源性仅为24 . 5 ，且不含有专利保护的保守序列和突变位点，显示了良好的开发应用潜力。
• The enzyme 5 - enolpyuvyl - 3 - phosphoshikimic acid synthase ( epsp synthase ; ec2 . 5 . 1 . 19 ) , encoded by anoa locus , is a key enzyme present in microorganisms and plants where it has a function in the biosynthesis of aromatic amino acids . glyphosate ( n - phosphonomethyl - glycine ) is an effective non - selective , broad spectrum , postemergence herbicide , which has been shown to inhibit epsp synthase activity in a competitive manner . glyphosate tolerant plants can be mediated by either overproduction of the target enzyme or by the presence of an altered enzyme
  植物和微生物芳香族氨基酸生物合成过程中的一个关键酶? ? 5 -烯醇丙酮莽草酸- 3 -磷酸合成酶( epspsynthase ； ec2 . 5 . 1 . 19 )由aroa基因编码，该酶受广谱灭生性、内吸传导型除草剂草甘膦的竞争性抑制，将epsp合成酶基因转入植物中可获得草甘膦耐受植株。
• The insert dna fragments are 7kb and llkb , respectively . two subclones that were designated pgr3h1 and pgr7h1 and can increase glyphosate resistance of e . coli jm109 up to 150mm glyphosate were constructed by subcloning the 2 . 4kb and 3 . 2 kb hind ? / psti fragments of pgr3 and pgr7 into the corresponding sites of pgem - 3zf and pbluescript . sequence analysis of these two subclones revealed a completely identical 1323bp open reading frame that encodes an epsp synthase
  以pgem - 3zf和pbluescript为载体，利用限制性内切酶hind和pst构建这两个克隆的亚克隆，从中分别得到两个草甘膦耐受亚克隆pgr3h1和pgr7h1 ，插入片段各为2 . 4kb和3 . 2kb ，对这两个亚克隆进行序列分析，发现二者均含有一个核苷酸序列完全相同的完整的epsp合成酶基因? ? aroa ，其核苷酸序列长为1323bp ，推导的epsp合成酶由441个氨基酸组成，两个亚克隆的草甘膦耐受浓度最大可达150mm 。
• Glyphosate is a foliar applied , broad spectrum , post - emergence herbicide capable of controlling annual and perennial grasses and dicotyledonous weeds . due to the similarity structure between glyphosate and phosphoenolpyruvate , glyphosate can inhibit epsp synthase by rivalrously uniting epsp synthase with phosphoenolpyruvate
  草甘膦是一种施用于叶片的、广谱的、非选择性的、后现性的有机磷类除草剂，它对于许多一年生和多年生杂草和双子叶植物都有极强的控制能力。
• Using this system , we have studied matrine - inhibittory effect and trifluoperation - neuroprotection effect in hippocampal slices , also discussed the mechanism of long - term potentiation using anesthetic rats . the experiment results showed that matrine can inhibit the hyperactivity induced by penicillin sodium in dosage by changing the relative parameters of field potential ; trifluoperation can alter ps change with the time , enhance the degree and the ratio of ps recovery , then minis the hypoxic injury ; high frequency stimulate can increase ps amplitude and epsp slope for long time , buildup the in / out function of nerve cells , and enhance synaptic plasticity
  结果表明，苦参碱能够剂量依赖性地抑制青霉素诱导的神经元顺向信号传导激活过程，使细胞外记录到的场电位各个参数发生相应改变；三氟拉嗪可以改变ps的时相变化，提高ps的恢复程度和恢复率，减小了神经元因缺氧引起的不可逆损伤；高频刺激( highfrequencystimulate ， hfs )可以长时间的增强ps的幅度和epsp的斜率，进而增强神经元的输入输出功能，增加了突触的可塑性。
• The common biosynthesis pathway of aromatic amino acids includes seven steps from dahp to chorismate acid . for the common pathway , 3 - dehydroquinate ( dhq ) synthase ( encoded by arob ) , 5 - enolpyruv - oylshikimate s - phosphate ( epsp ) synthase ( encoded by aroa ) , and chorisma - te synthase ( encoded by aroc ] are rate - limiting enzymes
  芳香族氨基酸的合成步骤有七步是共同的，亦即从dahp到分支酸的合成步骤，其中脱氢奎宁酸合成酶( arob ) 、 5 -烯醇式丙酮酰莽草酸合成酶( aroa )和分支酸合成酶( aroc )是此代谢途径的关键酶。
• A pair of primers was designed based on the conserved regions of other higher plants " epsp synthase through homology alignments . two dna fragments were first cloned from o . violaceus by performing prc which used o . violaceus genome as the template . one has 798 nucleotides and the other 1157 nucleotides , but they can encode the same amino acid sequence and have same extrons according to the gt - ag rule of characteristic sequence of enkaryoutic intron
  根据同源比较其它高等植物中epsp合成酶基因，找出该基因的保守序列并设计一对寡核苷酸引物，以诸葛菜的总dna为模板进行pcr反应，克隆出了两个epsps基因的片段，其中一条长为797bp ，另一条1157bp ，它们在genbank的登录号为： af440390 、 af440391 。