cytidylyltransferase造句

• Methods : in cultured lung explants without serum , the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity , nitric oxide ( no ) content , superoxide dismutase ( sod ) level , malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods . results : 1 . influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine , glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry , glu induced the changes of the ultrastruture of at ii iv cells
  方法：采用成年大鼠肺组织无血清培养，运用[ ~ 3h ] -胆碱掺入法测定ps主要脂质磷脂酰胆碱( pc )合成量； rt - pcr扩增检测肺组织中pc合成限速酶磷酸胆碱二胞苷酰基转移酶( cct ) mrna含量；透射电子显微镜法观察肺泡型上皮细胞和ps系统超微结构的变化；免疫组织化学染色检测glu的受体nmdar1亚单位的表达；生化测定肺组织乳酸脱氢酶( ldh )释放量和肺组织匀浆中一氧化氮合酶( nos )活性、一氧化氮( no )生成量、超氧化物歧化酶( sod )水平以及丙二醛( mda )含量。