accl造句

• The genome could be digested only by restriction endonucleases hind, accl and xbal, and was resistant to cleavage by a wide range of other restriction endonucleases
  病毒的基因组可以被hind、acc和xba酶切消化，但不能被其他所使用的限制性内切酶消化。
• In this study, a 1.7kb kpni fragment and a lacz gene expression cassette carrying the e . coli lacz gene under the control of sv40 promoter were inserted into the transfer vector pbdtk-uni ( a 277bp acci-accl fragment in the tk gene was deleted ) . the new transfer vector was called puni-lacz . the transfer vector puni-lacz and purified genomic dna of strain bartha-k61 were used to cotransfect vero cells using lipofectin transfection procedure
  本研究以呈ge~-表型的经典弱毒疫苗bartha-k61株为亲本株，在通用prv转移载体pbdtk-uni的基础上，在其多克隆位点中插入由sv40早期启动子控制下的lacz基因表达盒，同时将下游同源臂增加了一个1.7kb的kpni片段，使上下游同源臂的长度都超过了1kb，构建了一个新的转移载体puni-lacz。