t7rna聚合酶的英文
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"t7rna聚合酶"怎么读用"t7rna聚合酶"造句
英文翻译手机版
- t7 rna polymerase
- "聚合酶"英文翻译 polymerase; polyase
- "聚合酶" 英文翻译 : dna polymerase i, bacterialdna; dna polymerase ii, bacterialdna; dna polymerase iii, bacterialdna; dna polymerase α, eukaryoticdna; dna polymerase β, eukaryoticdna; dna polymerase δ, eukaryoticdna; dna polymerase ε, eukaryoticdna; dna polymerases: kinetics and mechanismdna; polyase; polysaccharase; trna polymerasetrna; tth dna polymerase tth dna; vent dna
- "dna聚合酶" 英文翻译 : deoxyribonucleic acid polymerase; dna-polymerase; taq dna polymerase
- "dna聚合酶i" 英文翻译 : dna polymerase i
- "ntp聚合酶" 英文翻译 : ntp poiymerase
- "pfu dna聚合酶" 英文翻译 : pfu dna polymerase
- "rna聚合酶" 英文翻译 : dna dependent rna polymerase; rna polymerase
- "taq dna聚合酶" 英文翻译 : taq dna polymerase
- "taqdna 聚合酶" 英文翻译 : taq dna polemerase
- "tth dna聚合酶" 英文翻译 : tth dna polymerase
- "vent dna聚合酶" 英文翻译 : vent dna polymerase
- "多a聚合酶" 英文翻译 : poly a polymerase
- "多聚聚合酶" 英文翻译 : polypolymerase
- "多糖聚合酶" 英文翻译 : polysaccharide polymerase
- "核仁rna聚合酶" 英文翻译 : nucleolar rna polymerase
- "核酸聚合酶" 英文翻译 : nucleic acid polymerase
- "核心聚合酶" 英文翻译 : core polymerase
- "聚合酶,有3" 英文翻译 : dna
- "聚合酶;多聚酶" 英文翻译 : polymerase
- "聚合酶;多糖酶" 英文翻译 : polyase
- "聚合酶反应" 英文翻译 : rna
- "聚合酶结构" 英文翻译 : rna polymerase structure bacterialrna
- "聚合酶类" 英文翻译 : rna polymerases
- "聚聚合酶" 英文翻译 : polypolymerase
- "依赖于dna的rna聚合酶,rna聚合酶" 英文翻译 : dna dependent rna poly-merase
例句与用法
- To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells . methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase
方法:根据端粒酶htert基因1573 ? 1591位的核酸序列,构建带t7启动子的部分双链dna模板,用t7rna聚合酶体外合成短链shrna 。 - Conclusions : the in - vitro method that partial double - strand dna with t7 wi l . - toi promoter sequence as template and synthesizing by t7 rna polymerase can product high yield , excellent purity shrna . lt is a convenient - , effective ^ low - cost method and fit for small rna synthesis and rna interference researches in ordinary laboratory
结论:以带t7启动子部分双链dna为模板,用t7rna聚合酶体外合成出的shrna产量较高,纯度较好,是一种简便、高效、低成本的短链rna的制备方法,适合于普通实验室用来进行短链rna的合成和rna干扰实验。 - 1 . expression of cry genes located in native plasmid in different flagella serotype strains to study cloning and expression of icps genes , an ecor i - f fragment of cryla ( a ) gene from pesi was inserted into pselect - 1 with t7 rna polymerase promoter in vitro . the plasmids of bacillus thuring fensisybt - 803 and ybt - 791 were analyzed by southern hybridization using an rna probe of ecori - f fragment and by pcr identification with cryl mixture primers
将cry基因的高保守区的cry a ( a ) ecog - f片段插入带有t7rna聚合酶启动子的质粒pselect - 1 ,获得了能在体外转录的rna探针载体pbpl - 1 ,用该载体制备的rna探针具有特异强,背景清楚,省时省力等优点,已成功地用于苏云金芽胞杆菌的分子生物学研究和特异菌株的筛选。 - Using pcr technology , a 2 . 4kb dna fragment , part of tryptopanase operon , containing a promoter , a regulator gene tnac located downstream of the promoter and a desired tryptopanase gene tnaa which can be expressed by the promoter from e . coli k - 12 , was cloned to pmd18 - t vector . the dna sequence is the same as which was published on science
为了证明质粒上的基因能表达出有活性的色氨酸酶,将这个dna片段克隆到pet28c质粒的bamhi和hind位点上,使该片段受t7rna聚合酶的启动子控制,然后转化噬菌体de3的溶源菌bl21 ( de3 ) 。 - To prove that the cloned dna fragment can express tryptopanase , a new plasmid pet28c - tnaa , in which the cloned dna fragment was located downstream of t7 promoter on pet28c was constructed and transformed into host bl21 ( de3 ) , a bl21 lysogen of bacteriophage de3 in which the only promoter known to direct transcription of the t7 rna polymerase gene is the lacuvs promoter , which is inducible by iptg
用iptg诱导表达t7rna聚合酶,以表达质粒上的目的基因。在葡萄糖存在的条件下,用常规方法发酵和诱导( 37 1mmiptg ) ,发现表达的蛋白质条带的分子量与理论上计算的分子量一致。但是发酵液中检测不到吲哚,表明虽然表达了目标蛋白,但表达的蛋白质没有酶活性。
其他语种
- t7rna聚合酶的阿拉伯语:تي 7 بوليميراز الحمض النووي الرايبوزي;
百科解释
T7RNA聚合酶(英语:T7 RNA Polymerase)是一种RNA聚合酶,分子量约99kDa。专门催化5'→3'方向的RNA形成过程。
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