Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells , we design a pair of particular primers , and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic . the expression plasmid was identified with pcr and dna sequencing . pbv220 - r hpf4 was transformed into e . coli dh5a , bl21 ( de3 ) and induced by increasing the temperature to 42 . we identified the expression protein by sds - page and western - blotting 目的1人血小板因子4 ( hpf4 )原核高效表达克隆的构建2重组hpf4的表达及分离、纯化工艺研究3重组hpf4的特性研究方法根据原核细胞表达真核蛋白的基因表达调控特点，设计合成一对特异引物，在pt7 - 7 - rpf4表达质粒的基础上，应用聚合酶链式反应( pcr )对其cdna进行改造，通过dna重组技术构建成重组hpf4原核表达质粒pbv220 - rhpf4 ，用快速pcr检测法、 dna测序分析，鉴定重组hpf4表达质粒的正确性。
In this study , the whole e2 genes of two strains of classical swine fever virus ( csfv ) isolated from guangxi ( gx ) were amplified by reverse transcriptase polymerse chain reaction ( rt - pcr ) method and seqenced . the e2 gene fragments of csfv were 1090 base pair in length and encoded 364 amino acid residues 研究采用反转录?聚合酶链式反应( rt ? pcr )技术对两株分别从柳州( gxlz )和南宁( gxnn )分离的广西流行猪瘟病毒( classicalswinefevervirus ， csfv )进行e _ 2全基因的扩增、克隆和测序。扩增片段长度为1090bp ，编码364个氨基酸残基。
Strain pseudomonas psuedoalcaligenese ys1 was capable of producing phas containing monomer of hb and mcl has in certain medium . phacl and phac2 , two key polyhydroxyalkanoates polymerase genes of pha biosynthesis were amplified and cloned from chromosomal dna of pseudomonas psuedoalcaligenese ys1 using pcr 本研究利用聚合酶链式反应( pcr )技术，从p . psuedoalcaligeneseys1染色体dna中扩增并克隆了调控短链与中链pha生物合成的两个关键酶基因： phac1 、 phac2基因。
Along with the rapid development of computer , communication and automation technologies , manufacturing equipment is having improving automation level , more complex structures and functions , and increasing potential fault possibilities and modes . further more , some fault may cause chain reaction , and the whole system fail 随着以计算机、通信、自动化为代表的信息技术的迅猛发展,制造设备的自动化程度越来越高,功能、结构日趋复杂,发生故障的潜在可能性和方式也在相应的增加,且一处故障就可能引起链式反应,导致整个系统的失效。
Based on the previous studies , the research in this paper was carried out , mainly including two parts as follows : ( 1 ) anammox bacteria and aerobic ammonia oxidizers were detected in situ in 6 sediment samples taken from jiangsu province . molecular techniques , such as fish , pcr , dna cloning and sequencing etc . were used for this purpose . ( 2 ) the continuous cultivation of anammox bacteria from sediment samples were studied , which provides experimental basis for the bioaugamentation of eutrophicated sediment applying anammox process 本论文在前人研究的基础上，开展了以下两个方面的工作： ( 1 )采用分子生物学技术荧光原位杂交( fish ) 、多聚酶链式反应( pcr ) 、 dna克隆和测序等对采自江苏省苏州市、东太湖、新沂河等6个底质样品进行了厌氧氨氧化菌和传统氨氧化菌的原位检测； ( 2 )探讨了以底质作为接种体进行厌氧氨氧化菌富集培养的可行性，为天然底质环境中厌氧氨氧化过程的强化，富营养化底质微生物修复的可行性提供一定的依据。
The target studied in this paper is one of the most important components in the ads . coupling the two innovative fields in the nuclear scientific and technological circles that are accelerator and sub - critical reactor . the physical function of this part is be as the neutron source , accepting the medium or high energy proton , breeding spallation reaction , arising the self - sustaining nuclear fission and generating the power 本文所研究的靶件作为该系统最重要的关键部件之一，耦合核科技界的两大创新领域-高功率质子加速器和次临界堆，该靶件的物理作用主要是接受中、高能质子，发生散裂反应，产生中子源，引发持续链式反应，产生能量。
A conservative motif , recognized by proteinases of potato virus y , was inserted between nib and ppiv , which will release functional ppiv from the fused protein after infection by potato virus y . then , plant expression vector pnpa was constructed by ligating the fusing gene and pbi121 , which is knocked out gus gene 以马铃薯y病毒的复制酶( nib )基因为模板，通过聚合酶链式反应获得nib基因，并在nib基因末端保留了在病毒基因组中nib与外壳蛋白( cp )基因相衔接的保守序列。
