The cdna encoding growth hormone ( gh ) peptide was amplified by reverse transcription polymerase chain reaction ( rt - pcr ) method using isolated total rna as template 应用逆转录-聚合酶链式反应( rt - pcr )技术克隆得到编码草鱼生长激素( cgh )的基因cdna ，并定向克隆到puc18载体上。
Polymerase chain reaction is a rapidly developing and widely used dna amplification technique , which is widely applied in life science and other related fields 聚合酶链式反应( polymerasechainreaction ，简称pcr )技术是发展很快、应用很广的体外扩增基因片断技术，在生命科学研究及诸多相关领域已经得到了广泛应用。
A pair of primers containing sph i and hind iii restriction sites were designed , according to the poifn - a gene in ddbj / genbank . then poifn - a gene was cloned from porcine genomic dna by pcr 根据ddbj genbank基因库中已登录的猪干扰素基因序列，设计了含sph和hind酶切位点的一对引物，采取聚合酶链式反应( pcr )法，以猪基因组dna为模板进行了poifn克隆。
The series include : hcg test kit , afp test kit , cea test kit , em antibody test kit , as antibody test kit , etc . the series of pcr kits the series of pcr kits has very high sensitivity , excel distinctiveness and accurate results 基因扩增检测试剂系列是采用聚合酶链式反应技术研制而成，具有配套试剂全标本处理简便灵敏度极高特异性强结果准确等优点，适用于医院临床检测和科研。
The analysis of oxidation mechanism indicates that the principal causes of thermal oxidation resistance are as follows : rare earth elements have a function to discontinue autooxidation chain reaction and the formed complex structure can hinder the oxidation resistance 经过氧化机制分析，认为抗热氧化效应的原因主要是稀土元素具有中止自动氧化链式反应的作用以及形成的络合结构有阻碍氧化的立体效应。
Newcastle disease virus ( ndv ) strain 695 , a thermostable nature avirulent strain , were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid . referred to the reported sequence of f gene , a pair of primers were designed and synthesized . f gene of ndv b95 strain was amplified by rt - pcr , the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method 利用从国外引进的新城疫热稳定性天然弱毒b _ ( 95 )株接种spf鸡胚繁殖病毒，经处理后提取病毒的基因组rna ，参考国内外发表的ndv融合蛋白基因序列，设计一对特异性引物，经反转录聚合酶链式反应( rt - pcr )扩增出约1700bp大小的特异性片段，将此片段回收纯化后，利用t - a克隆技术将其克隆到pgem - t - easy克隆载体中，再转化大肠杆菌jm109感受态细胞，转化后经分子量比较、 pcr鉴定和酶切分析筛选阳性克隆。
All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene . this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc ) 所有的样本通过聚合酶链式反应?限制性片段长度多态性方法( pcr - rflp )对ahsg基因的第7个外显子内的sac多态性位点进行基因分型，该多态性位点为238号氨基酸密码子中间的碱基c到g的替换，使苏氨酸( thr ， acc )变为丝氨酸( ser ， agc ) 。
Rapd ( random amplified polymorphic dna ) , which bases on the polymerase chain reaction ( pcr ) , is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms . the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna , and a small number of fragments will be amplified when the primer anneals on each strand over a length range . if sequence variation is present at the priming site , then a fragment may not be amplied , so the dna polymorphic can be detected Rapd (随机扩增多态性dna )技术是二十世纪90年代发展起来的一项dna分子多态性检测技术，它建立于聚合酶链式反应( pcr )技术基础之上，利用随机合成的寡聚核苷酸序列为引物(一般为10个bp ) ，分别与dna的两条单链结合，在dna聚合酶的作用下，对基因组的特定区域进行pcr扩增，其电泳结果为不同大小和数目的dna谱带即rapd图谱，可反映基因组相应区域的dna多态性。
One pair of specific primers pai1 and pai2 was designed according to the sequence of ha gene of aiv h5 subtype reference strain . the fragment of ha gene was amplified by rt - pcr using its genome rna as template . the obtained target fragment was cloned into the vector pmd18 - t and th en e . coli was transformed 以其基因组为模板，根据禽流感h5亚型参考毒株ha基因序列，设计合成了一对特异性引物pai1和pai2 ，经反转录-聚合酶链式反应( rt - pcr )扩增出本毒株的cdna ，所得片段连接到载体pmd18 - t后，转化大肠杆菌。
A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin . four oligonucleotide primers , based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1 , were designed to amplify specific dna fragment from different viruses 依据apmv - 1融合蛋白( f )基因裂解位点的核苷酸序列与其毒力的相关规律，分别设计合成了四条寡核苷酸引物，建立了一个可迅速检测不同禽源apmv - 1并可鉴定强、弱毒株的逆转录酶?聚合酶链式反应( rt - pcr )技术。
