Expressed sequence tag ( est ) analysis was carried out to study the molecular mechanism of salt tolerance for polygonum sibiricum laxm . . a cdna library was constructed from polygonum sibiricum laxm . treated by salt using stratagene cdna synthesis & gigapack iii gold cloning kit 为了研究西伯利亚蓼耐盐的分子机理，使用stratagene的cdna合成和gigapack gold包装试剂盒构建了盐( nahco _ 3 )胁迫下西伯利亚蓼的cdna文库，随机选取重组克隆测序，用表达序列标签( est )方法研究盐胁迫下西伯利亚蓼基因的表达。
By using this method , multi - glycoproteins were detected from the tubes in two distinct areas . purification of glycoprotein using same condition of sds - page , same proteins were loaded , stained both with coomassie blue and with gel code glycoprotein staining kit respectively . under the condition , we successfully recovered glycoprotein bands from sds - gel by passive diffusion of proteins 糖蛋白的纯化选择了相同样品、相同的电泳条件，用考马氏蓝染色、糖蛋白试剂盒染色，分别对同一糖蛋白样品对照鉴定，用蛋白质的被动扩散回收法，成功地从sds胶上快速地纯化多种茶树叶糖肽。
Since there are more things in the bud tissue of the male flower , such as phenolic compounds , polysacchrides , proteins and some other secondary metabolites , three methods used to isolate rna are tested in this assay , which are the enzyme digesting methods of ctab , ctab - dna and sds - dna 克服了常规方法和试剂盒无法提取出富含酚类化合物、多糖和一些尚无法确定的次级代谢产物的白桦花芽组织rna的障碍，提出了3种白桦雄花芽组织rna的提取方法： ctab 、 ctab - dna酶消化法和sds - dna酶消化法。
At the same time , 10 serum sample were detected by elisa kit made in abroad and agp . the result of three elisa detection shows : s / p of 8 detected serum higher than 0 . 2 is positive ; s / p of 2 detected serum is negative . the result accords with the result of agp detection 最后，再将自制vp1板用自配试剂检测其活性，同时用自制板和国外试剂、国外elisa试剂盒、琼脂扩散试验检测同样10份血清作比较，三组elisa检测结果，其中有8份被检血清s p (抗体水平)大于0 . 2 ，为阳性； 2份被检血清为阴性。
Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using . triplix kit . a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction . the ss - dna was reversely transcripted from total rna . double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i , the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro . a high titer and high recombinant ratio of cdna library was constructed 构建恶性疟原虫海南株红内期cdna表达文库提取红内期恶性疟原虫海南株总rna ，直接以总rna为模板使用cdna文库构建试剂盒，首先反转录合成ss一dna ，再扩增合成ds一dna ( cdna ) ，对扩增产物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶试剂盒纯化、回收20obp以上的片断，经与载体连接再用蛋白包装物包装后形成未扩增文库，最后扩增完成恶性疟原虫海南株红内期cdna表达文库的构建。
