Hertz biotech offers a range of bio - separation products , which includes magnetic microspheres , nucleic acid purification kits , magnetic separators , porous silica microspheres , liquid chromatographic packings and columns 磁性分离装置、磁性微球、超纯核酸提取试剂盒、色谱填料、色谱柱、羧基荧光素、氨基酸保护试剂等系列产品。
Viral rnas were extracted from virus - infected allantoic fluids using qiaamp mini - extraction kits . after reverse transcription , cdna was amplified using specific primers for each gene segment 用qiagen的rneasyrna提取试剂盒按说明提取，提取的rna逆转录合成cdna ， cdna为pgr反应扩增的模板，特异性的引物是针对各个基因片段设计的。
Chemical : 17 - estrogen , charps and aebsf was purchased from sigma company in usa . progesterone was purchased from xianju pharmaceutical factory in china . pcr kit , from takara company in japan 2主要试剂： 17p一雌二醇、 6 rps与3f （美国sma公司人孕激素（仙居制药厂厂pcr试剂盒（日本takara公司x其它常规试剂均为国产分析纯。
Purified glycoprotein was detected by gelcodeglycoprotein staining kit , and the result indicated that it was a glycoprotein , because two glycoprotein bands appeared in gel , with their molecular weights 63000 and 54000 respectively 糖蛋白染色鉴定试剂盒鉴定结果表明： cona - sepharose4b亲和层析的茶树叶蛋白是糖蛋白，在sds胶上出现了两条糖蛋白带，其分子量分别为63000和54000 。
The products cover the fields of the study of molecule biology , expression of protein , from cell culture to layer purification , from antigen antibody , the whole set reagent kit to the microorganism culture and the research of theactivity of the biology 其产品覆盖了生命领域从分子生物学研究、蛋白表达、细胞培养到层析纯化，从抗原抗体、成套试剂盒到微生物培养及其生物活性研究等相关试剂几近所有过程。
2 . the genomic dna purification method is different slightly in different species of plants . we had optimized the efficient quick extraction method of ctab , sds and adopt suitable kit and extracted the different plant genomic dna successfully suited to pcr and real - time pcr system 2 、不同类型的植物提取基因组dna的方法略有差异，本研究优化了ctab 、 sds快速提取法，并选择了磁珠吸附试剂盒法，成功提取了适合于pcr反应的植物基因组dna 。
The qualitative and quantitative detection method of gmo is established for the first time in china . now the kit for this method has been produced . this technique will replace the traditional detection method and become the main technique of gmo detection 本论文在国内首次建立了转基因产品荧光pcr定性、定量检测方法，并与深圳匹基生物公司合作推出配套试剂盒，该技术即将取代常规的pcr -凝胶电泳、 pcr - elisa等方法，成为转基因检测领域的主导技术。
In the experiment , the first instar housefly larvae " metallothionein mrna expressing reach maximur . after induced by cd ( 2mm ) for 48 hours , then total rna was extracted with guanidinium isothiocyanate , and mrna was isolated by the polyattract mrna isolation systems surplied by promega company 试验中采用2mmcd ~ 2对家蝇初孵幼虫诱导48小时，使其体内金属硫蛋白mrna表达达到高峰，在液氮中将其研磨成粉末后采用异硫氰酸胍法提取总rna ，再用promega公司提供的小量分离mrna试剂盒纯化mrna 。
Methods : 1 ) 12h after irradiation , the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp . 2 ) the content of mda , the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation . 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation 具体方法为： ( 1 )照射后12h ，收集nih3t3细胞，用流式细胞仪检测各组细胞的细胞周期， pcr - sscp检测抑癌基因p16的变化； ( 2 ) nih3t3细胞照射后立即收集细胞和细胞上清，用试剂盒测量mda含量和sod 、 gsh - px的活性并观察其变化； ( 3 ) western免疫印迹和点杂交法检测照射2h后的各组hela细胞中基质金属蛋白酶- 2 ( mmp - 2 )的表达变化。
After electrophorised on 1 % agarose gel , the pcr production was purified with agarose gel dna extraction kit . the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a . a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones . positive clone was identified by three ways : endonuclease digestion , pcr and sequencing . the result showed that the cloned sequence coincides with the designed sequence . this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit . the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ) . after 12 - 16 hours of culture , several colnes appeared on the plate . some positive clones were selected to extract their plasmid . these plasmids were digested by nco i and xho i and indentified by pcr . a contraction , mstnd - pet28a was generated . the result showed that the cloned sequence coincides with the designed sequence F _ 1长38bp ， r _ 1长36bp ，其它片段均40bp长， f _ 1和r _ 1片段两端分别加上限制性内切酶nco和xho的识别位点序列。用成对单链片段进行延伸反应，然后用其他单链片段作为引物，进行pcr扩增，用dna快速纯化回收试剂盒回收所得254bppcr产物，与pmd18 - t载体连接、转化dh _ 5 。受体菌感受态细胞，利用蓝白斑遗传学筛选法筛选阳性克隆，提取其质粒，采用nco和xho双酶切鉴定，获得了254bp的片段；用pmd18 - t载体上的特异引物rv - m和m13 - 47进行pcr鉴定，获得300bp的片段。
