缺失突变

• We clone a 1 . 3kb promoter sequence of the homologous gene in arabidopsis by pcr . this promoter is shown to direct the specific expression of the reporter gene , b - glucuronidase ( gus ) , in trichomes of arabidopsis . promoter deletion analysis reveal that the region from - 300 - - 1 bp is sufficient to direct trichome - specific expression
  对其进行缺失突变，构建5个缺失表达载体转基因拟南芥，叶片gus定量测定分析表明- 300bp ? - 1bp序列就可以指导gus基因在表皮毛细胞中特异表达，说明这段序列可能含有指导此启动子在拟南芥表皮毛细胞进行特异表达的核心序列。
• The level of cytosolic free calcium in gp mutants is not different . adding exogenous purified cam to the cultural medium increased the pollen germination , tube growth , and cytosolic free calcium concertration in both wild type and ga mutant . however , the former was more sensitive to exogenous cam than the latter
  外源钙调素(花椰菜和拟南芥11亚型， 10一7m )处理能够提高野生型及gpol缺失突变体中的游离钙离子水平，但对野生型花粉细胞游离钙离子水平的激活效应高于gpal缺失突变体。
• ( 2 ) deletions of four different lengths were conducted using pcr amplification with the information of sequence of pdf 1 . 2 gene promoter . these mutated promoters were fused to the gus reporter gene and introduced into tobacco plants . the results of gus activity driven by these different constructs showed that the promoter sequence between positions - 300 and - 243 ( containing a gcc box and a g box - like sequence ) was an essential ja - responsive element region to activate the expression of gus reporter gene , and the - 300 bp position was defined as the boundary of the minimal functional promoter in response to ja signaling
  ( 2 )对该启动子的5 ’端进行不同长度的缺失突变，突变的启动子与gus构建的融合基因在烟草中受meja诱导的瞬时表达结果表明，该启动子中- 300 - 243bp区域(有一个gccbox和一个gbox - likesequence )是其应答meja处理所必须的区域，并将- 300bp作为该启动子应答ja信号的最小区域的边界位置。
• Here we found g proteins also function in leaf , silique development and the yield of pollen microspore . we observed several traits or characters in the offsprings of gpal , agbl null mutation and gpa1 overexpression lines and found that the width of mutants " lamina is larger than that of the wild type , whereas the lamina length , petiole length and rosette diameter is smaller than the wild type , the ga overexpression lines is different from the mutants ; the silique length and the pedicel length is larger in mutants than that of wild type , and slightly smaller in overexpression lines than the control ; the morphometric character in silique tip is different in gpal from agbl mutants ; the yield of pollen microspore is larger in null mutants than wild type whereas smaller in overexpression lines
  实验中我们跟踪观察了多代异三聚体g蛋白a亚基超表达转基因植株及a ， p亚基缺失突变体的表型特征，发现突变体的叶片宽度大于对应的野生型，叶片长度，叶柄长度及莲座直径小于野生型，而超表达植株的上述某些特征与突变体相反; gp时突变体的长角果长度，花梗柄部长度大于野生型，而超表达ga植株种英则略小于对照; gpal突变体长角果尖端未出现咭乙i突变体的特征: gpal ，口gbl突变体花粉生成量大于野生型，而超表达ga植株的花粉生成量则略小于对照。
• ( 3 ) on the basis of the deletion analysis , three substitution mutants ( ml : 6bp sequence upstream of gcc box m2 : gcc box and m3 : g box - like sequence ) by pcr were designed to isolate the essential ja - responsive element . transgenic tobacco plants containing promoter substitution constructs were generated by agrobacterium - rnqdiaied leaf transformation . loss - of - function experiment , using transient expression analysis of gus reporter genes , confirmed that gcc box act as an essential element to respond ja signaling in pdf1 . 2 promoter
  ( 3 )在缺失突变的基础上，通过对gccbox及其相邻的上下游六个碱基进行取代突变，将突变启动子与gus构建融合基因，在烟草中受heja诱导的瞬时表达结果表明， h1和m3的突变对该启动子应答ja信号的影响很小，而m2 ( gccbox的突变)则几乎使该启动子应答ja信号的功能完全丧失，所以gccbox是该启动子中应答ja信号的必需元件。
• In this paper , an important cis - acting element within the promoter of pdf7 . 2 , which is activated by jas , was analyzed , and the interaction of this element with relevant factors ( jerfs ) was also studied . the results were as fellows : la ja responsive cis - acting element within the promoter of pdf 1 . 2 was investigated via comprehensive mutant analysis . ( 1 ) a strategy based on cdna sequence was used to amplify the promoter ofpdf1 . 2 gene with arabidopsis gdna as template
  本文对受jas诱导的pdf1 . 2启动子的顺式作用元件及其与jerfs的相互作用进行了系统研究，取得如下结果： 1 、通过对pdf1 . 2启动子的缺失突变分析和取代突变分析，对该启动子应答jas信号的顺式作用元件进行了较详细的探讨。
• After obvious cytopathogenic effects developed , virus - contained supematants were harvested , and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal . single blue plaques were picked , and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification . the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain
  该载体与具有高度感染性的bartha - k61株基因组dna通过脂质体加plus法共转染vero细胞，采用甲基纤维素固定病变， x - gal染色，经过10代蓝斑纯化获得了一株稳定表达lacz基因的ge tk基因缺失突变株，命名为rprv - lacz 。
• Eiav virions were observed by electron microscope from donkey leukocytes infected by virus derived from eiav - pok8 . 2 - his . these results demonstrate that changing the eiav s2 gene ( inserting small foreign gene fragment ) does not appear to affect replication properties in target cells in vitro
  本研究通过soe法巧妙地对s2基因引入突变，插入his标签，成功地获得了标记感染性分子克隆，证明s2基因缺失突变株在体外巨嗜细胞上培养不影响其复制力，为野毒株和疫苗毒的鉴别诊断打下基础。
• The result of blastx shows that one orf is extracellular serine protease precursor gene ; 3 orfs function as regulatory genes ; 5 orfs are involved in secretion pathway ; 2 orfs are related to production of lps ; and the annotation functions of the other 3 orfs are not clearly related to extracellular protease prouduction . marker exchange method was used to study the relationship between the production of protease and the 3 orfs . a deletion mutant of xcc _ 4463 was constructed successfully
  Orf注释表明，共中一个基因为胞外蛋白酶结构基因， 3个基因与合成调控有关， 5个与转运分泌有关， 2个与lps合成有关，其余3个orf的注释功能与胞外蛋白酶的关系未见报道，为研究这3个orf与胞外蛋白酶产生的关系，采用同源双交换orf缺失法进行了进一步验证，成功地构建了xcc _ 4463缺失突变体，所得缺失突变体经检测胞外蛋白酶减少，在寄主上致病性降低。