A sds - iso - propanol method suitable for tea plant , which was plentiful of tea polyphenols , had been developed using a modification of chen darning ' s method from different sample storage conditions such as fresh , dry and frozen shoots . it was a quick , easy , economical and effective method . the tactics were as follows : before the cell nuclear membranes were decomposed , the tea polyphenols and proteins etc . were removed 该法提取缓冲液使细胞维持一定的渗透压，研磨时使细胞核基本保持完整；在细胞核被裂解之前去除细胞质中的茶多酚、大部分蛋白质和rna ；而后用sds裂解细胞核，异丙醇或乙醇沉淀dna ，这样能经济、快速和有效地从富含茶多酚、茶多糖等次生物质的茶树新梢中提取基因组dna 。
In this study , the suitable parameters for the introduction of plasmids phz1358 and pset152 into s . nanchangensis ns3226 from e . coli were tested for developing an conjugation system for s . nanchangensis ns3226 . a dnd gene cluster , which encodes an unknown modification system for 5 hvidans 1326 and renders its dna susceptible to site - specific double - strand dna cleavage during electrophoresis was conjugated from e . coli into s . nanchangensis ns3226 将克隆在整合型载体pset152上的变铅青链霉菌1326的dnd基因簇通过接合转移导入野生型南昌链霉菌ns3226中进行异源表达，观察到接合转移子的dna获得了在含fe ~ ( 2 + )的电泳缓冲液中电泳时降解的表型。
The optimized parameters of high - frequency in bacillus thuringiensis plasmid - free mutant bmb171 by electroporation was studied . it showed that a highest electro - transformation frequency could be obtained , when sg solution was used as the buffer , and a 10 . 0 kv / cm of field strength , one time of pulse as well as a growth phase of recipient cells at the exponential phase ( od650nm value was 0 . 2 - 0 . 3 ) were selected 对用电脉冲法转化苏云金芽胞杆菌受体菌bmb171优化条件的研究结果表明，采用sg溶液作电脉冲缓冲液，用10 . 0kv cm的脉冲场强和1次电脉冲( 4 . 6ms ) ，以及采用对数前期( od _ ( 650 )约0 . 2 0 . 3 )收获的受体菌，可以达到最高转化频率。
Western blot analysis we treated 200 fertilized eggs in a series of steps including lysing them in l0ul of lysis buffer ( 50mm tris - hcl ph 7 . 5 , 250mm nacl , 5mm edta , imm dtt , 0 . 1 % triton , 50mm sodium orthovanadate , looug / mg pmsf and tpck , 50ug / ml tlck , 1 ug / ml leupeptin pepstatin and aprotinin ) , frozing them below - 70 , and then thawing them at room temperature for 10 min 2 . westem印迹将处理组及对照组小鼠一细胞g2期受精卵各200个取出，转移到ep - pendoff管中， 5000甲m离心4分钟，弃去上清，加入ro川粉碎缓冲液，充分振荡混匀，在液氮中经过2一3次的冻融循环，迫使卵细胞裂解，加人等量的样品缓冲液沸水中煮5分钟，用于westem印迹分析。
The electrochemical study showed that the interaction mode is mainly intercalative binding in ph 7 . 4 phosphate buffer solution . the uv - vis spectroscopic study further demonstrated the above results . through the electrochemical parameters such as charge transfer coefficient and standard electron transfer rate constant ks in the absence and presence dna , it was found that the reaction of aloe - emodin with dna forms an electrochemical active supramolecular complex 本文对姜黄素的研究结果表明,在0 . 1m磷酸盐缓冲液( ph3 . 0 )中,姜黄素于玻碳电极上存在可逆的单电子转移过程,据此,本文建立了以差示脉冲伏安扫描法检测姜黄素含量的新方法。
Material and methods normal rats of male sd were divided into young , adult , and aging groups . preparation of samples for light microscopy : animals were anesthetized by peritoneal injection of 6 % chloral hydrate ( 0 . 5ml / 100g body weight ) . perfusion and fixation of animals were carried out by a common procedure : 37 normal saline 50 - 100 ml and then 4 % paraformaldehyde pbs 100 - 400ml were perfused through the left ventricle of the heart , the whole procedure was lasted for about somin . the entire brain was dissected out and dipped in the fixative solution for 12h at 4 . brain pieces targeted were choosen and then passed the graded alcohols for dehydration , dipping into paraffin for embeding , and reshaping the pieces 2 ）磷酸缓冲液100400m ， 30分钟灌注完毕，取出整脑，在上述固定剂oc ）内后固定12小时。切取观察部位脑块，然后，进行梯度酒精脱水，浸蜡，包埋，修块，石蜡连续切片（德国leica石蜡切片机人切片厚度still ， zlllll ，蛋自甘油载片捞片， 60c烤箱过夜，二甲苯脱蜡，梯度酒精置换，浸水， h六染色，梯度酒精脱水，二甲苯透明，中性树脂封片。室温风干后，显微镜观片， olympus万能显微镜照相。
The results showed : the best additive water to wheat bran solid medium was 35 percent . a . niger an01001 produced high activity phytase when it was cultivated at 30 ? from 90 to 120 hours , and obtained the highest enzyme activity at 114 hours . phytase activity was effected by different buffer and ph , enzyme activity was high at ph 5 . 5 and 6 对该菌的产酶条件进行研究的结果表明：麸皮固体培养基的最适加水量为35 ； 30培养90 120h之间产酶都比较高，在114h酶活最高；在提取时不仅ph对酶得率有影响，而且不同提取液所要求的最适ph也不同，用乙酸缓冲液提取时，当ph为5 . 5 、 6 . 5时酶活较高，进行水提取时，在ph7 . 5时酶活最高；利用cacl _ 2溶液进行提取的浓度达到3时，酶活最高。
Purification and characterization of phytase from a . niger an 01001 a . niger an01001 was inoculated on solid media and cultivated at 30 for 5 days . proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5 . 5 ) . the molecule weight of the phytase protein was determined as about 78kd by sds - page . the purification procedures include ammonium sulfate precipitation , deae - cellulose ion - exchange chromatography , gel electrophoresis and electroelution 3 .植酸酶的分离纯化及其性质研究黑曲霉ano1001经固体发酵，用缓冲液抽提后，经硫酸按沉淀， deae一纤维素离子交换层析，聚丙烯酞胺凝胶电泳和电洗脱等纯化步骤获得的植酸酶，用sds一page检测为一条均一谱带，其分子量约为78kd 。
Transmission electron microscopy : the testis was cut into fragments ( 1mm x 1mm x 1mm ) , fixed in 2 . 5 % glutaraldehyde made up in 0 . 1 m phosphate buffer ( ph 7 . 2 ) , postfixed in 1 . 0 % oso4 , dehydrated in a progressive ethanol and acetone solution , embedded in epon812 , sectioned with lkb ultramicrotome , and stained with urangl acetate followed by lead citrate , then observed with h - 600 microscopy and photographed 透射电镜样品以2 . 5戊二醛( ph7 . 2 ， 0 . 1m lpbs缓冲液配制)和1锇酸双固定，酒精和丙酮系列脱水， epon812包埋， lkb型超薄切片机切片，醋酸铀和柠檬酸铅双重染色， h - 600透射电镜下观察并拍照。
We have sifted 103 medicinal plants , roughly identified 17 plants might contain antifungal proteins . antifungal protein was purified from cassia sophera linn , by extraction , fraction with ( nh _ ( 4 ) ) _ ( 2 ) so _ ( 4 ) , cation - exchange chromatography of cm - sepharose ff xk 26 , the first cation - exchange chromatography of mono s and the second one , followed by gel filtration of superose 12hr 对茳芒决明进行了抗菌蛋白的分离纯化：经粉碎、磷酸缓冲液浸提、硫酸铵沉淀、 cm - sepharoseffxk26阳离子交换层析、两次monos阳离子交换层析、 superose12hr分子筛层析可得到具抗真菌活性的蛋白。
