Scanning electron microscopy : after fixed in 2 . 5 % glutaraldehyde made up in 0 . 1 m phosphate buffer ( ph 7 . 2 ) , the tissue of testis was post - fixed in 1 . 0 % oso4 , dehydrated in a progressive ethanol solution , dried and sputter - coated with gold , then observed with kyky - 1000b microscopy and photographed 扫描电镜样品以2 . 5戊二醛( ph7 . 2 ， 0 . 1m lpbs缓冲液配制)和1锇酸双固定，酒精系列脱水，干燥，真空离子溅射仪喷金， kyky - 1000b型扫描电镜观察并拍照。
5 . prepare the oligonucleotide microarray add 16 prepared probes into 384 - pole plate , meantime , add p2 , low homologic probes and probe buffer in the same concentration as positive control , blank control and negative control respectively . spot microarray as designed by the spotting machine 5 .寡核昔酸微阵列的制备以同等浓度的反义链引物咫作为阳性对照，以探针缓冲液作为空白对照，以无关探针作为阴性对照，与制备好的16条寡核昔酸探针，同时加人384孔板。
Liposome is an effective nuclide delivery agent for neutron capture therapy . in this paper liposomes containing encapsulated gadolinium complex were prepared and characterized . the influence of formulation factors such as ph , ionic strength , buffer , and storage time upon the stability of liposomes was investigated . the uptake rate constant and its concentration dependence of lgd in tumor cells were compared in vitro with that of gd complex itself . the results indicate that the uptake rate of lgd in tumor cells increases to eight times as much as that of gd - edta , but the release rate of gd from tumor cells containing lgd is remarkably lower than that from the tumor cells containing gd - edta . the results reveal that lgd would be a potential drug for neutron capture therapy of cancer 制备和表征了包埋gd - edta的脂质体，测定了ph ，离子强度，缓冲液组成及温度对gd - edta脂质体的影响，比较了gd - edta脂质体和gd - edta被肿瘤细胞摄入的动力学曲线。结果表明， gd - edta脂质体在37和生理条件下最稳定，肿瘤细胞摄入gd - edta脂质体速率是gd - edta的8倍，而释放gd的速率， gd - edta脂质体远远低于gd - edta ,这些结果提供了脂质体包埋gd - edta作为钆中子俘获治疗药物的可能性。
Among the three methods used in the experiment of dna extraction , only ctab , adding pvp in the dna isolation step , had effectively reduced the disturbance from fiber or other plastids and extracted suitable genomic dna as template for rapd process . pcr amplifications were performed in a final volume of 25 mm3 containing 0 . 5 units taq polymerase , 2 通过在抽提缓冲液加入2的pvp 、并用异丙醇和乙醇沉淀基因组dna等改良措施， ctab法能避开大量纤维、多糖的影响，有效地从不同属种的棕榈科植物的幼嫩叶片中提取并纯化了适合rapd的基因组dna 。
In the first part , both behavioral responses and fos expression in dorsal horn induced by peripherally injected formalin were observed . animals were divided into three groups ( n = 5 ) : one of them was control with injection of vehicle ( hepes ) into the plantar aspect of the left hindpaw ; and two of them were experimental groups with injection of selective mglur , and mglur5 antagonists , cpccoet or mpep , respectively 实验组动物山西医科大学的一侧后脚掌分别注入20仍级硕士学位论文mg1ur 、 、 mglur 、的拮抗剂cpccoet ( 1oon二01 ) / m尸ep ( 50nmol ) ，对照组则在一侧脚掌注入缓冲液hpeps (两种拮抗剂均用hpeps溶解) 。
In contrast , n - acetyl - d - galactosamine , sucrose , d - glucose , glycogen , d - mannose , l - fucose and fetuin had no inhibitory effects on hemagglutination . so the lectin activity in the humoral fluids of amphioxus is sh group - dependent and is specifically inhibited by various d - galactosides . also , the humoral fluids obtained from e . coli - injected amphioxus showed increased agglutinating activity against human b and o , rabbit , grass carp and toad erythrocytes , but not against human a and chick erythrocytes , hinting that there might be two types of lectins in amphioxus humoral fluids 条纹斑竹鳖血清对悬浮在egta一mg一gvb缓冲液中的正常的兔血红细胞、绵羊红细胞、及部分哺乳动物、鸟类、两栖类和鱼类等代表动物的血红细胞具有溶血活性;在gvbz十存在时对抗体包被的绵羊红细胞具有溶血活性，而在edta一gvb缓冲液中不论是对正常的兔红细胞或其他脊椎动物的红细胞还是对抗体包被的敏感绵羊红细胞都没有溶血活性。
Method to collect respectively 180 unrelated males " venous blood 500ul , who lived in shanxi province , 120 unrelated mongolians " venous blood 500ul , who lived in the inner mongolia autonomous region , and the blood is anticoagulant with edta , then to extract dna by using the method of phenol - chloroform after ingested by proteinase k at 56 and amplify the dys413 site by using pcr 方法采集180例山西汉族和120例内蒙古蒙古族男性无关个体静脉血各500ul ， edta抗凝，用tkml液反复洗涤至无色，加入2蛋白酶k缓冲液180ul ，蛋白酶k ( 20mg ml ) 20ul ，在56消化至液体清亮为止，用酚-氯仿法抽提dna ， pcr扩增dys413位点， 6非变性聚丙烯酰胺凝胶电泳， 1硝酸银染色分型。
The fungus age , enzyme system , osmotic stabilizer , ca2 + concentration influenced on the formation of the protoplast have been confirmed . the result show that 16 - 18hr fermention , 32 1 . 0 % cellulase plus 1 . 0 % snailase confected by pba ( phosphate blend ammonium chloride ) contant 0 . 2 % ca2 + , acted 3 hr is optimal for the protoplast production of aspergillus . niger 分别采用单因子法和正交实验法考查，认为16 18hr菌龄的菌丝体， 32 ,用含0 . 2 ca ~ ( 2 + )的pba高渗缓冲液配制的1纤维素酶与1蜗牛酶的混合酶酶解3hr ，得到的原生质体数最多。
75 % was injected in the tibialis anterior muscle of mice , three days later , cea dna vaccines were injected , each side 50 jag , with different adjuvant ( iss or pil ~ 2 ) . there are 5 mice in each group . the control group was injected with pbs buffer in a dose of 50 ju 1 的布比卡因注射入mn几小鼠胜前肌，在2郑州大学医学院2002研究生毕业论文癌胚抗原a疫苗构建及佐剂对其兔疫效应的影响注射后第3大分别接种浓度为ing ul的ceadna疫苗及相应佐剂口ss或gil 《厂每侧50ug ，每组5只小鼠，对照组注射50yi的pbs缓冲液。
The presence of complement - like activity in the humoral fluid of amphioxus was examined . the complement classical pathway ( cp ) activity is assayed by determining the amount of serum required to hemolyse a given number of sensitized sheep red blood cells ( srbc ) , whereas the complement alternative pathway ( ap ) activity measured in the same manner , but by using rabbit red blood cells ( rarbc ) instead of sensitized srbc 文昌鱼体液可溶解悬浮在10mmol legta - mg - gvb缓冲液中的正常的兔血红细胞、绵羊红细胞、及部分哺乳动物、鸟类、两栖类和鱼类的代表动物的血红细胞；而在gvb ~ ( 2 + )缓冲液中时对抗体包被的绵羊红细胞却没有溶血活性。
