The expression of subunits b , h and c were greatly higher in young tissues ( 4 - week - old ) than those in old tissues ( 10 - week - old ) , which clearly indicated that the expression of v - h + - atpase subunits had relationship with plant development . the salt enhanced expression of v - h + - atpase subunits in roots , shoots and leaves in both young arid old s . s 对生长四周，幼嫩的盐地碱蓬植株及生长10周处于衰老期的盐地碱蓬植株进行400mmol lnaci处理，分离其根、茎、叶总rna进行northern分析发现盐地碱蓬液泡膜h “ atpaseb 、 h 、 c亚基基因表达与植株发育有密切关系。
The main results of the experiment are as follows : 1 the comparison of the stress effects of nacl and na2co3 on suaeda salsa l . the growth of suaeda salsa could be promoted by certain concentrations of nacl but inhibited markedly by na2co3 the change in root vitality was not significant under nacl stress but significant under na2co3 stress 1nacl和na _ 2co _ 3对盐地碱蓬胁迫效应的比较300mmol l以下的nacl能促进碱蓬的生长，而na _ 2co _ 3在浓度不很大时就能显著抑制碱蓬的生长。碱蓬在nacl胁迫下根系活力变化不显著，但在na _ 2co _ 3胁迫下，碱蓬根系活力则显著下降。
2 . effect of nacl treatment on the subunits expressions and activities of h + - atpase and h + - ppase from suaeda salsa northern blot analysis indicated that the transcription of v - h + - atpase subunits b , h and c were obviously up - regulated by nacl stress . the transcripts of mrna of b and h subunits in 400 mmol / l nacl - treated plants were significantly higher than those in control during 0 - 120h and the mrna levels of b , h and c subunits were also increased under 100 and 400 mmol / l nacl treatment 400mmol lnacl处理盐地碱蓬植株0 - 120h发现盐胁迫明显诱导了盐地碱盐胁迫下盐地碱茨液泡膜质子泵衷达分析及过量系达印mhxaw对拟甫芥耐盐性的影响蓬液泡膜h ” atpaseb 、 h亚基基因表达； 100 、 400mmol lnaci处理盐地碱蓬植株，分析其叶片液泡膜h ”
The transgenic arabidopsis plants co - overexpressing of ssnhxi and a vp1 were also been analyzed to examine salt tolerance of the transgenic plants . the results were shown as follows : 1 . cdna cloning and sequence analyzing of subunits b , h and c of tonoplast h + - atpase in halophyte suaeda salsa full cdna sequences encoding tonoplast h + - atpase subunits b , h and c were isolated from the cdna library of halophyte suaeda salsa through est analysis 盐地碱蓬液泡膜h ~ + - atpaseb 、 h 、 c亚基基因克隆及序列分析利用est随机挑取克隆测序的策略从盐地碱蓬叶片cdna文库分离得到编码盐地碱蓬液泡膜h ~ + - atpaseb 、 h 、 c亚基的全长cdna序列。
The first two methods are the most commonly used for soybean transformation . the other methods have not been optimized for soybean , and are therefore less efficient and not often used . previously , we isolated ssnhxl ( encoding a vacuolar na + / h + antiporter ) from s . salsa , and further research was done through the overexpression in arabidopsis thaliana , it could help us to elucidate the salt tolerance of halophyte 本实验室成功的从盐地碱蓬中克隆到位于液泡膜上的ndm ”反向转运蛋白基因ssnhx ，转化拟南芥并对该基因的功能进行了分析，发现在拟南芥中过量表凶亥基因可以巳著提；削6物的灿处性。
Previously , we have constructed a 400mmol / l nacl - treated cdna library of s . salsa and acquired 1000 ests ( expressed sequence tags ) by the large - scale partial sequencing of randomly selected cdna clones . in this research , we isolated a cdna that maybe encode an aquaporin ( s . salsa plasma membrane intrinsic protein , sspip , bf023789 ) located in plasma membrane of s . salsa . then we analyzed the sequence characterizations , genomic structures and transcriptional levels under salinity stress 本实验室构建了400mmol lnacl处理的盐地碱蓬( s . salsa )地上组织的cdna文库，随机挑取单克隆进行测序，获得了可能编码盐地碱蓬质膜水通道蛋白( s . salsaplasmamembraneintrinsicprotein ， sspip )的cdna ，其序列号为bf023789 。
The cdna of subunit b was 1470 nucleotides long coding for 489 amino acids with a conservative atp binding site " 324 - sgsit - 328 " and a predicted molecular mass of 54 . 29 kda . amino acid sequence alignment analysis suggested that the v - h + - atpase b subunit of s . salsa had high homology with other reported v - h + - atpase subunit b . the h + - atpase subunit h from s . salsa was a hydrophilic protein with 465 amino acids and a predicted molecular mass of 52 . 8 kda , which was encoded by a cdna with 1398 nucleotides in orf . blast analysis indicated that the h + - atpase subunit h from s . salsa had a high amino acid sequence identity with those coming from plants , but had relatively low sequence identity with those coming from other species 盐地碱蓬液泡膜h ~ + - atpaseb亚基是由1470个核苷酸编码的长达489个氨基酸的多肽，分子量约54 . 29kda ，存在保守的atp结合序列“ 324 - sgsit - 328 ” ，与其他物种来源的v - h ~ + - atpaseb亚基具有较高的氨基酸序列相似性； h亚基为亲水多肽，开放阅读框长达1398 - bp ，编码465个氨基酸残基，分子量约52 . 8kda ，与植物来源的液泡膜h ~ + - atpaseh亚基具有较高的氨基酸序列相似性，而与其他物种来源的v - h ~ + - atpaseh亚基同源性较低； c亚基开放阅读框为495 - bp ，编码164个氨基酸残基，分子量约16 . 6kda ，为一跨膜多肽，存在四个可能的跨膜区。
We analyzed their sequence characterizations , genomic structures and transcription levels under salinity stress . the results indicated that ssvp showed highest homology to the vacuolar h + - ppase ( cvp ) gene from chenopodium rubrum and phylogenetic analysis indicated that ss vp and cvp share a cluster , which showed that they might be more similar in evolution . southern blot analysis showed that there was more than one copy of ssvp in the suaeda salsa genome 本实验从400mmol lnacl处理的盐地碱蓬地上部分构建的zap - cdna文库中克隆了编码mapkk的全长cdna ( ssmapkk ， ay093683 )和编码液泡膜h ~ + - ppase的部分cdna ( ssvp ) ，据已知序列设计引物利用pcr方法获得编码盐地碱蓬液泡膜h ~ + - ppase的全长cdna 。
Cluxtalx analysis indicated that the v - h + - atpase b , h and c subunits from halophyte s . salsa had high amino acid sequence identity with subunits from mesembryanthemum crystallinum , which is an inducible cam species as its growth rate is maximal at moderate salt concentrations . sequence alignment results suggested that the evolutions of v - h + - atpase subunits b , h and c were involved in environmental adaptation and these subunits of v - h + - atpase might have an important role in responses of plants to salt stress Clustalx分析表明盐地碱蓬液泡膜h ~ + - atpaseb 、 h 、 c亚基氨基酸序列与来源于兼性cam植物冰叶日中花液泡膜h ~ + - atpase的相应亚基具有最高的序列一致性，表明植物液泡膜h ~ + - atpaseb 、 h 、 c亚基的进化可能与环境刺激有关并且可能参与了植物对盐胁迫的响应。
