Using western blot analysis , it revealed that neither peg nor nacl treatment could initiate accumulation of dehydrins in plbs . ii . studies on enhancement of drought and freezing tolerance by gene transfer of cbf1 主要结果如下: 1 .将c丑尸z基因与cal城v35s启动子融合构建植物表达载体pbnzi一cbfi ，以农杆菌介导的叶盘法转化烟草，获得转化植株。
This gene , artificially put under the control of camv 35s , was introduced into tobacco with the aid of agrobacterium , and the transgenic plants obtained were identified by gus staining , pcr and southern blot analysis 将arge与组成型启动子camv35s相连，构建植物表达载体，通过农杆菌介导转化烟草。经过gus染色、 pcr及southern鉴定获得了转基因植株。
Subsequently , the recombined plasmid was transformed to the strain jm109 of escherichia coli . , which was picked up and was ligated to pbi121 . as a result , the plant expression vector of pbi121 - ubic was constructed successfully 提取质粒，连接重组到经相同酶切的ti质粒pbi121上，构建了植物表达载体pbi121 - ubic ，并将其转入根癌农杆菌lba4404中。
We confirmed the correct construction by pcr and restriction enzyme analysis . in this research , hypocotyls were used as the explant and several factors affecting genetic transformation of carrot mediated by agrobacterium tumefaciens were studied 利用vp7基因和质粒pbi121上相同的单克隆位点，将vp7基因定向克隆到植物表达载体pbi121上，构建了pbi121vp7表达载体。
The fusing gene was transformed into leaf tissue of tobacco cultivar nc89 . more than 100 plants were obtained . transgenes were confirmed to be inserted into tobacco genome of r0 generation by pcr and dot - blotting analysis 通过kpn位点与成熟番木瓜蛋白酶的cdna连成一个融合基因，并插入到pbi121的原gus位点，构建成nib -番木瓜蛋白酶融合基因的植物表达载体pnpa 。
Modified cry1ac and cry1ie gene had also been cloned into plant expression vector p3301 , to construct plasmid p3301ubiac and p3301ubiie in which two genes were under the control of maize ubiquitin - 1 promoter respectively 进一步将crylac ， crylie基因构建到真核表达载体p3301中，构建植物表达载体p3301ubiac和p3301ubiie 。在这两个表达载体中，两个基因分别受ubiquitin启动子的调控。
A plant expression vector was constructed by following method : s gene of 1bv and 35s promoter was cut from recornbinant pbi121 , and then the fragment was inserted into the multiclonal sites hind / bamh i in the plasmid pcambia1305 . 1 通过从重组质粒pbi121上切下s基因(连同35s启动子)片段，将该片段定向克隆到pcambia1305 . 1质粒的多克隆位点hind 、 bamh之间，构建了一种植物表达载体。
So a plant expression vector with two insect - resistant genes - pbbbast - pin ii - sip was constructed . a plant expression vector - pbbbast - sip with one insect - resistant gene was also constructed by inserting the sip gene into the plasmid pbbbast 实验以蜘蛛杀虫肽基因为目的基因，将其转入质粒pbbbast - pin中，从而构建含有pin和sip两个抗虫基因的双价植物表达载体? ? pbbbast - pin - sip 。
In the study , sound system of tissue culture and genetic transformation of carrot , cv , xinheitianwuchunrensheng , are created . " a plant expression vector pbihlf , based on pbin121 , was constructed then transferred into carrot 本研究在对胡萝卜组织培养及遗传转化体系进行优化的基础上，构建了人乳铁蛋白基因植物表达载体，在农杆菌介导下，首次把人乳铁蛋白基因导入胡萝卜，获得了转基因植株。
The main results was summarized as follows . 1 constructed plant expression vectors with rip gene of barley we constructed four vectors altogether and two was intermediate vector ( pur and pjar ) , the other two was plant expression vectors ( pcar and p3301rip ) 主要研究结果如下： 1构建了核糖体失活蛋白基因植物表达载体本试验共构建了4个载体，其中包括2个中间载体pur 、 pjar和2个表达载体pcar 、 p3301rip 。
