Construction of plant expression vector containing human interferon gene and expression in ginseng calli 人干扰素基因植物表达载体的构建及其在人参愈伤组织细胞中的表达
Construction of plant expression vector and analysis of herbicide - resistance and salt - tolerance of transgenic tobacco 植物表达载体构建和转基因烟草抗除草剂及耐盐性的分析
Construction of an expressing vector for antisense rna - ribozyme chimeric dna sequence against tomato acc synthase gene and transformation of tomato 核酶嵌合基因植物表达载体的构建及对番茄的转化
Transformation of cucumber plant expression vecor pbet and pbemt were transferred to cucumber and pcr positive transgenic plants was obtained 黄瓜遗传转化将上述结果中的植物表达载体pbet和pbemt转化黄瓜获得pcr阳性植株。
A binary plant expression vector with osg6b " driving report gene gus was constructed and transferred into tobacco via agrobacterium tumefaciens mediation 将克隆的启动子与报告基因gus相连，构建植物表达载体，通过农杆菌介导转化烟草。
The aim of this research is to establish a transgenic system for flower plants and investigate the functions of mapkk in transgenic plants in the future 本研究构建了野生型、突变型mapkk基因植物表达载体，并用冻溶法将其导入了根癌农杆菌lba4404 。
Plant defensin afp ( 238bp ) was amplified with six long complementary primers after two rounds of pcr amplification and plant expression vector peafp was constructed . 3 通过合成六条长链引物，经两次pcr扩增，获得了238bp的植物防御素afp基因全长，并构建了植物表达载体peafp 。
( 2 ) at translation level plant mutual sequence of starting translation aaca was added to start codon of t - pa gene by pcr ampliation and plant expression vector pbet was constructed ( 2 )在翻译水平上通过pcr扩增的方式在t - pa基因起始密码子处添加了植物翻译起始共有序列aaca ，构建了植物表达载体pbet 。
To identify the function of ttom1 during the viral infection of the plant host , a 2 . 6 kb genomic dna near the 5 " end of ttom1 , was used to generate antisense ttom1 transgenic tomato plants 用pcr的方法扩增ttom1基因的近5 ’端2 . 6kb的dna片段，构建反义植物表达载体pbit1 - 2 ，通过三亲结合的方法将其导入到农杆菌eha105中。
2 . obtaining of mcema ( modified cema ) and plant defensin afp gene mcema ( 141bp ) was amplified with pucspcema plasmid as template and p5 , p4 as primers , and plant expression vector pemcema was constructed Mcema和植物防御素基因的pcr合成以经测序验证的spcema为模板，用p _ 5和p _ 4扩增得到了全长141bp的mcema ，并构建了植物表达载体pemcema 。
