Williamson indicates that there are bilateral multilateral and hybrid marginal organizes which are called market web organize , namely enterprise alliance . recently , study on enterprise web is abundant 威廉姆森指出在企业和市场之间还存在着双边、多边和杂交的中间边际组织，可称之为准市场网络组织，就是我们常说的企业联盟。
The results of our study of last year found that the secretion levels of ley and mmp - 9 of the same morphology were different . by using highly sensitive dot blotting method , the difference can be clearly detected 我室在近期研究发现不同的胚胎虽然形态上看没有区别，其le ~ y 、 mmp - 9的分泌却不相同，这种差异可以用点杂交的方法检测到。
To detect the patients and carriers of the fragile x syndrome , various analytic methods could be used . one is cytogenetic diagnosis to observe the expression of the fragile site fraxa located at xq27 . 3 对脆x综合征的诊断，可以通过细胞遗传学方法观察分析x染色体相应脆性位点的表达情况，或是通过southern印迹杂交的方法直接分析cgg重复的扩增突变。
By following the cross fundamentals of biology gas use crossover operators to generate new individuals . that is some portion of alleles in two matched parents are exchanged by some means to pass down to their offsprings 遗传算法仿照生物学杂交的原理使用交叉算子来产生新个体，即在两个配对的染色体中按某种方式相互交换其部分基因，从而形成新的个体。
Reverse northern hybridization indicated that p1a5 gene was over - expressed in bract compared with that in leaf . this expression pattern was further conformed by rt - pcr . sequence analyses revealed that it is a partial sequence of a lipid transfer protein gene 通过抑制性差减杂交的到克隆pias ，反向northern杂交表明它在玖桐苞片中优势表达，即在苞片中的表达水平远远超过在处于同时期的叶片中的表达水平。
Was first processed by dgd embedment and embedment - free technique and general technique for em morphology . a perinuclear structure consisted of interacted filaments we called lamina - like structure was observed . then using western blot assay , we found a lamin - like component band of 68kd protein in the three - step - fractionated cells . to investigate the distribution of the lamin - like protein in cells , a immunofluorescence experiment for in situ hybridization was designed using goat anti - lamin protein antibodies as the probes . the results revealed that the positive reactivity presented at different part of the cells . the perinuclear cross - actions were distinct , and cross - action with the oral apparatus and the cortex were also obtained 本文以dgd包埋去包埋技术对草履虫的核纤层通过透射电镜和免疫荧光分子杂交等技术进行了观察。结果显示，在核周存在由10nm纤维组成的核纤层免疫荧光结果表明，在核周呈阳性反应，并在其表皮口器等部位呈交叉的阳性反应蛋白分子杂交的反应带在68kd处呈阳性反应。
According to the southern hybridization results , 43 bacillus strains were divided into several groups , 22 strains were chosen randomly from all these groups for sequencing of aii gene . it was found that the nucleotide acid sequence of aii genes showed 85 . 4 % - 100 % homology and amino acid sequence of aii proteins showed 8 8 . 1 % - 100 % ho mology 在southern杂交的基础上，将检测的43个菌株分组，对其中22个菌株的aii基因进行dna序列测定，结果表明，不同菌株aii基因的dna序列同源性为85 . 4 ? 100 ；而aii蛋白的氨基酸序列同源性为88 . 1 ? 100 。
The chimeric gene was introduced into arabidopsis through the method of vacuum infiltration which is widely used in arobidopsis tansformation . transgenic plants were screened by means of kanamycin resistance and further identified by genomic pcr and western blotting , through the phenotype observation of transgenic plants , the speculation that apoplast calmodulin may play a certain role in regulating the development and growth of plants was made 最后，融合基因被插入二元载体plbj21 ，通过电击法转入农杆菌gv3101 ，真空渗入法转入了拟南芥，转基因拟南芥通过kan抗性筛选而得到，并进一步进行了基因组pcr鉴定和western杂交的鉴定。
The results showed that the compatibility indexes were obviously higher when the male sterile lines were used as the female parent than when their respective maintainer counterparts were used as the female parent , indicating the male sterile cytoplasmic effects on interspecific cross incompatibilities in cruciferae 结果表明：三种类型的不育系均有一致的趋向，即用不育系和保持系与同一亲本杂交的亲和指数，不育系作母本的明显高于以保持系作母本的组合，表明十字花科种间杂交亲和性具有雄性不育细胞质遗传效应。
The distribution and amount analysis of these bacteria in different layers of core sediment indicated that there was an intact cycle that coupled sulfur metabolism with methane metabolism existed in this area , which may be the microbial response to the environment because there was seldom similar bacteria detected from " manganese nodule " area sediment by dna - dna hybridization with specific oligonucleotide probe and 16s rdna clone library analysis 而16srdna克隆文库分析和dna - dna杂交的结果表明“结核”区沉积物中这两类细菌数目很少，说明“暖池”区沉积物中的微生物群落结构特征是对环境因素的一种响应，同时也可能是影响该海区深海及海洋环境的一个重要因素。
