Results : gain 5 hyhridoma cells secreting anti - p24 antibodies , in which 2 - e4 is selected for further hybridization and its ascites fliud antibody titer is 1 : 1600000 细胞株作为再杂交的备用细胞，其腹水型抗体的elisa效价为1 ： 160万。
Thomas in brno ? once in austria - hungary , today part of the czech republic ? mendel revealed the laws of inheritance by experimenting with pea hybrids in the walled monastic garden 孟德尔在修道院围墙内的花园进行豌豆杂交的实验，因而发现了遗传的定律。
The cross made , the hybridizer then waits as the newly fertilized ovules develop into viable seeds . or not . the majority of attempted crosses fail 做好交配后，育种者于是开始等待新受精的子房发育，产生可育的种子，或是一无所获。大多数情况下杂交的尝试会失败。
The ascites fliud titer of the other hybridoma s2 for further hybridization is 1 : 16000 by blood agglutination . the isotype of 2 - e4 is igg2a , and s2 is iggs 用于再杂交的另一细胞株为本室保存的分泌抗人a型红细胞单克隆抗体的杂交瘤细胞株s ； ，其血凝效价为l ： 16000 。
" the sudden popularity of the puggle , a dog bred from a pug and a beagle , has started up a long - running argument among animal lovers about mixing breeds and species . “由哈巴狗和小猎犬混种而来的哈巴小猎犬的突然流行,已经在动物爱好者中间再度激起了那场关于品种杂交的长期争论。 ”
In attempting to domesticate the quagga ? a zebra - like , south american member of the horse family ( now extinct ) ? he was forced to breed a male with a female arabian mare 他在试图驯养斑驴（一种马，产于南美，外型象斑马，现已绝迹）时，这匹雄性斑驴与一匹雌性阿拉伯母驴交配，杂交的结果正常。
( 3 ) because the expression signal of map2a was hard to be detected by the northern hybridization , we combined rt - pcr and southern hybridization to determine its expression ( 3 )鉴于map2a基因的表达量较低，我们采用rt - pcr结合southern杂交的方法，研究了map2a基因在苹果不同组织中的表达状况。
Use northern blot , it was only detected in developing seeds . we use rt - pcr to amplify the expressing signal , and we found it can be detected in growthing leaves and stems 直接用northern杂交的方法只能在发育中的种子里检测到，本文采用rt - pcr的方法放大表达信号，发现可以在发育中的叶片和茎中检测的到。
Isolating mechanism structural , physiological , behavioral , genetic , geographical , or other factors that restrict the interbreeding of one population with another , hence restricting gene flow 隔离机制：由于结构、生理、行为、遗传、地理或其它因素限制了一个种群与另一种群杂交的机制，从而限制了基因的流动。
Efforts to impregnate the two purebred washington rabbits have been unsuccessful so far , he said . next month , biologists should know how many females are pregnant and how many crossbred babies will be born this year 下个月，生物学家就将得知，该计划中有多少雌性侏儒兔已经怀孕以及今年将诞生多少杂交的兔宝宝。
