Methods 1 ) statistic methods including factorial experiment was carried out to optimize the major conditions for sample management , and the feasible negative and positive control for fcm analysis of cd62p expression were check out 方法1采用浓度梯度法优化gprp浓度条件，采用析因设计优化凝血酶浓度和37孵育时间条件，寻找最佳阴、阳性对照。
Feverish fruit fly larvae , warmed in a toasty lab chamber , are giving cornell researchers a way to watch chromosomes in action and actually see how genes are expressed in living tissue 在一个温暖的实验室隔间中孵育的果蝇幼虫非常兴奋，它们给康奈尔大学的研究者指出了条康庄大道，让他们可以观察活动中的染色体并且可以实时看到基因在活体组织中是如何表达的。
Corp . was founded in july 2000 . originally located in the tsing - hua university s business incubation center , it was successfully hatched in 2004 . it is now an r d company specializing in analog ic design 翔科技股份有限公司frontand tech . corp .于2000年7月成立，原本位于清华创新育成中心内， 2004年孵育成功，离开自立，是一家以类比ic设计为主的研发公司。
Pretreatment with l - arginine ( l - arg ) before incubation with fac could not reverse the inhibition of relaxation response to ach completely . ( 2 ) in endothelium - intact aortic rings , l - arg could relax the pe preconstricted vessels （ 2 ）正常含有内皮的主动脉环在pe预收缩后，给予不同浓度的l afg可引起血管舒张；铁孵育后， l afg引起的舒张幅度显著减小（ p 0刀5 ） 。
Then the mixture was incubated at 45 ? for 5h , then lyophilized . 6 . the combination between macrophage and oligochitosan labeled with amac : macrophage cells were co - cultured with amac labeled oligochitosan for 2min , 5min , 30min : the control group was treat 不同浓度的壳寡糖对巨噬细胞胞浆游离钙离子浓度的影响：巨噬细胞于37oc 、 5 co ， ’孵箱中培养24小时，冲洗后加入10wtol ifioo 3 am100ill ， 37避光孵育40分钟。
2 . gln , tau , and gly effluxes from rat hippocampal slices are calcium - dependent under normal oxygen supply condition , while the other amino acids release are calcium - independent 3 . 10 min of hypoxia treatment increased amino acids release in normal acsf medium except gin and ala 3c ”依赖性和非ca ’ ”依赖性释放正常供氧条件下去除脑片孵育液中的”后，海马脑片孵育液中gn 、 tl 、 gly水平与对照组相比分别降低38 4 ， 12 7 ， 14
We did the same steps for three times , so we could get the extraction using the steps mentioned . laemmli sample buffer was added to the extracted protein , which were then boiled for 5 min . the protein samples were separated by 12 % sds - page and transferred to nitrocellulose membrane 样品蛋白经12 % sds一聚丙烯酞胺凝胶电泳分离后，转印到硝酸纤维素膜上，与第一抗体4孵育过夜，经tbs洗涤后，再与第二抗体室温孵育2小时， ttbs充分冲洗后，显色观察。
Materials and methods the mouse , golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation , respectively , and ar was induced by progesterone after capacitation , then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method . the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf , then the fertilization rate was observed . the 1 - cell , 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin , then the rate of blastocysts was recorded 方法取小鼠精子10份、金黄地鼠精子6份、人新鲜精液标本10份及人冷冻精液标本9份，分别与不同浓度内毒素共孵育进行体外获能和孕酮诱导的顶体反应，应用金霉素和dna结合的荧光染料hoechest33258双重荧光染色法检测精子的获能率和顶体反应率；小鼠体外受精实验的精卵结合环节培养液中加入不同浓度的内毒素，观察受精情况并记录受精率；取小鼠1 -细胞胚胎、 2 -细胞胚胎和去卵透明带2 -细胞胚胎，与不同浓度内毒素共孵育进行体外培养，观察体外发育情况并记录囊胚率。
And at the beginning of each macro procedure , operation check processes were inserted to minimize the user interference . a standard comet assay was conducted using the improved comet analysis macro , with the intoxication by 100 / / m hydrogen peroxide to mononuclear cells of mouse peripheral blood for 0 , 10 , 20 , 40min respectively to get different dna damage grades 同时，本研究应用改进后软件进行标准彗星试验检测，以100 m过氧化氢染毒小鼠外周血单个核细胞，并经历0 、 10 、 20 、 40min不同时间的孵育，获得不同损伤级别的细胞，并以改进后的软件进行分析。
At present , in an attempt to stimulate specific antitumor immunity , experimental models and clinical studies are currently evaluating the potent antigen - presenting capacity of dc combined with single or multiple tumor antigen epitopes . however , there are several problems in utilizing pulsing dc with synthetic immunodominant peptides from identified antigens . 1 ) the potential induction of tolerance ; 2 ) the need to determine the patient ' s hla haplotype , the limitation of therapy to patients whose tumors express defined specific tumor antigens in the context of the correct hla phenotype , the unavailability of peptides for all hla haplotypes ; 3 ) the lack of cd4 help cell - related epitopes for most antigens ; and 4 ) the ctl resulting from such protocols have a good in vitro capacity to kill peptide - pulsed target cells but only a modest capacity to kill tumor cells 但是，学者们发现这一疗法存在着如下的缺陷：单一ctl表位抗原肽的应用其抗肿瘤作用弱于多种肿瘤抗原的联合应用，且有诱导免疫耐受的潜在危险，有时反而会促进肿瘤的生长；事先需对患者的hla单倍型进行鉴定，以确定ctl表位与hla单倍型是否匹配，目前尚缺乏能与所有hla单倍型相匹配的ctl表位，从而限制了这一疗法的应用；这一疗法所产生的ctl在体外能有效杀伤经肿瘤抗原肽共孵育过的靶细胞，但对肿瘤细胞的杀伤能力较弱：这种l表位抗原肽缺乏cd4汀h细胞相关的表位，因此，不能诱导有效的th细胞免疫应答。
