In this paper , our method was performed in two separate steps . first , microarray data was normalized to eliminate experiment - wide systematic effects and then differentially expressed genes were prejudged under a loose standard via a single gene model . second , these differentially expressed genes were confirmed under a stricter standard to control the false positive via a multi - gene model 本文提出的方法主要分为两步来进行：首先，将芯片数据通过噪音过滤消除大的试验系统误差，然后在一个比较宽松的标准下通过单基因模型初步判断差异表达基因；其次，用多基因模型分析这些初定的差异表达基因以便在较严的标准下控制假阳性。
In this paper , three human dna fragments were coinjected into mice in order to study the influence of uncoding large dna fragment on hsa or hfen1 gene ' s integration efficiency , to explore methods to produce high expression of human serum album animals , to find out how to establish multi - transgenic animals in a way of saving time and money , to provide experience and reference to others . it mainly contains the following aspects : firstly , according to the sequence of human serum album gene cdna in genebank , one primer was designed 本实验以小鼠为研究对象，采用两种基因? hsacdna 、 hfen1和一种非编码大片段dna进行共注射，旨在探索非编码大片段dna对hsa和hfen1基因整合的影响及建立提高hsa基因整合效率的转基因动物的方法，同时，探索多基因转基因动物建立的策略，以便寻求一种既节省时间，而又简单经济的转基因方法，为以后的研究提供一点的探索性经验和参考。
Abstract ： based on present plant gene engineering techniques , some strategies to develop transgenic crops expressing multiple genes by constructing a multigenes expression vector or a number of expression vectors and then making one or more transformations or co - transformation are discussed and the prospects are described as well in this paper 摘要本文探讨了在利用植物基因工程技术的基础上，通过构建多基因或者多个表达载体，进行一次、多次基因转化或共转化方法获得转多价基因作物的一些策略，并进行了展望。
Based on present plant gene engineering techniques , some strategies to develop transgenic crops expressing multiple genes by constructing a multigenes expression vector or a number of expression vectors and then making one or more transformations or co - transformation are discussed and the prospects are described as well in this paper 本文探讨了在利用植物基因工程技术的基础上，通过构建多基因或者多个表达载体，进行一次、多次基因转化或共转化方法获得转多价基因作物的一些策略，并进行了展望。
The gene qutb was cloned to pbv220 , qdhase was expressed and had specific activity in e . coli . 3 . construction of co - expression recombinants of multigenes in a single plasmid pbv220 and its optimization according to different combined modes , six co - expression recombinants , each gene of which recombinant is under control of lambda phase prpl or pl promoter , were constructed and expressed 单质粒pbv220多基因串联表达重组子的构建及优化以不同方式组合，构建了含有各自启动子prpl或pl的二基因arog 、 qutb串联表达重组子四种和三基因arog 、 qutb 、 arob串联表达重组子二种。
This review focuses on the conceptions and technologies of omics , and puts forward the central contents and omics docking for polygenic inheritance tumor to reveal the role of molecular changes at different stages of polygenic inheritance tumor at multidisciplinary and multilayer level 现主要阐述组学对接研究相关的概念和技术平台，提出针对多基因遗传性肿癖的组学对接战略中最主要的策略和研究内容，从多学科、多层次揭示多基因遗传性肿瘤不同发病阶段中的分子变化规律。
