The rotary cell culture system ( rccs ) from synthecon is a new development in bioreactor technology which enables the cultivation of differentiated three dimensional cultures that mimic the structure and function of parental tissues 凭借其生长和维持聚集物那样的活组织的能力，细胞和组织科学家有条件对新生和外植细胞的生长和相互作用的基本过程进行研究。
2 . according to the effect of combination of different hormone concentration in the medium on callus formation and shoot induction of tomato cotvledons , we defined mso + 2 . 0mg / l ba + - 0 . 2mg / l iaa as optimum differential medium 根据番茄子叶外植体在加有不同激素浓度配比的培养基上愈伤组织分化和芽再生的情况，确定最佳分化培养基为ms _ 0 + 2 . 0mg / lba + 0 . 2mg / liaa ； 3
Rate of cluster buds induced by different gingkgo explants is not the same . among them , cluster buds can be obtained directly from stems and mostly from axillar bud , but the amount is little and differentiation rate is only 20 % 银杏不同外植体对丛生芽的诱导不同，银杏茎段培养可以直接得到丛生芽，但数量极少，而且大部分属于腋芽萌发，分化率20左右。
In this experiment , the immature embryos were used as explant to induce calli and bt gene was tranfered into mo17 , 81162 , ji 846 and 7922 by the agrobactrium - mediated . we have gained the positive plants determined by pcr 本论文以东北地区骨干玉米自交系幼胚为外植体，采用农杆菌介导法将bt基因导入了mo17 、吉846 、 81162及7922 ，并获得了pcr检测呈阳性的再生植株。
This review includes the following subjects : induction frequency of haploids depend on genotypes , developmental stage of explants , temperature pretreatment of the explants , medium composition and the conditions of cultivation 迄今己有9个科21种植物用这一方法诱导出单倍体植株，植物的基因型、外植体的发育程度、接种前的预处理、培养基和培养条件等均能影响诱导率的高低。
Two main contents were concluded in this paper : the plant regeneration and genetic transformation . some effective factors were studied in tissue culture in order to establish a quick , high frequency regeneration system of genetic transformation for long ya lillium 本研究包括两个主要内容：龙牙百合的组织培养和遗传转化。以龙牙百合为材料，组培研究表明，鳞片叶是组培的适宜外植体。
Cotyledon and true leaves of stem mustard were cultured on ms medium supplemented with different concentrations of various cytokinins ( ba , cppu , tdz or kt ) alone or in combination with naa or iba , and the adventitious buds regeneration rate was evaluated . 以榨菜子叶、真叶为外植体进行离体培养，用ba 、 cppu 、 tdz和kt等细胞分裂素和生长素naa 、 iba组合诱导子叶、真叶再生不定芽试验。
Floral organs have been successfully induced from the regenerated floral buds of ffyacinthus orienralis l . by precisely controlling exogenous hormones in the medium . under high concentration of cytokinin and auxin , the regenerated floral bud produces only tepals 以风信子花被片为外植体，通过控制培养基中细胞分裂素和生长素的浓度，可诱导花芽的再生和控制产生花器官的种类，建立起离体花器官定向控制系统。
2 hybridization results show that hag1 is specifically expressed in stamens and carpels in vivo ; hag1 mrna was first detected in cultured explants at day 5 in the medium containing high levels of cytokinin and auxin , which could induce floral regeneration in vitro Rna分子杂交结果表明： hag1特异性地在植株上花的雄蕊和心皮中表达：在高浓度激素培养诱导花芽形成过程中，第5天开始检测到外植体中有该基因的mrna 。
The callus was induced and proliferated using young leave as explant , which comes out at early spring . in this paper , the author discussed the induction effect and the proliferation effect by using different kinds of hormone and different physical - chemical factors 本文以山茶初春新生枝条的幼叶为外植体，采用不同的培养基，探讨了不同的植物生长调节物质对愈伤组织的诱导和不同的理化因子对愈伤组织的增殖效应。
