Before planting into outdoor soil , the tube - plants should be acclimated in green house for 15 ~ 20days firstly , the surviving rate could up to 93 % 并进一步优化了转化体系：以愈伤组织为外植体，转化效率较理想；用叩值
Explantation the culture of isolated tissues of adults or embryos in an artificial medium for the study of maintenance , growth , and / or differentiation 外植：在人工培养基上培养由成体或胚分离出的组织，以研究其维持、生长及/或分化。
Therefore , the distance of 9 . 0cm and bombardment for two times each treat are appropriate parameters to transform heterogeneous gene into foliage explant of h . brasiliensis 因而，靶距离为gcm 、转化两枪是基因枪转化橡胶树叶片外植体较合适的参数。
With additional inter - fixation , the construct has an effective biomechanical stability , as mean while the long - term bone fusion can be reached use Aht avb兼顾内植骨与外植骨融合，临床可有效恢复椎节高度，患者可早期下地，同时可满足远期骨性融合的需要。
Hzf1 has no or low level mrna accumulation in the eplants cultured without hormones , and its expression were found in all organs in the plant 当外植体在天外源激素的培养基上培养时， hzf则低水平表达。在植株上该基因在不同的器官中均表达，但表达水平不同。
To gain the maximum transformation efficiency , the explants should be pre - cultured two days , co - cultured four days and infected with agrobacterium tumefaciens for ten minutes 在所试验范围内，叶盘外植体的转化效率以预培养2天、共培养4天、菌液感染10min最高。
The explants of for root , hypocotyl and cotyledon were cultured . among them the hypocotyls from 20d or 30d seedings was the best for callus forming and anthocyanin production 以20d或30d龄的无菌苗下胚轴作外植体，效果好，愈伤组织生长量大，花色素苷的含量也高。
Soybean cotyledonary nodes were transformed with s gene of ibv via a . . tumefaciens - med ated transformation . the highest transformation rate of 6 . 7 % was detected in the present experiment 以子叶节为外植体，在抗性筛选培养基上大多白化死亡，有少数生长出芽，转化频率最高为6 . 7
The suitable concentration of hyg to screen for resistant shoots was 50 mg / l . the kind of antibiotics to inhibit the growth of agrobacterium eha105 was cef , of which concentration was 250 mg / l 以洋桔梗叶盘为外植体，通过根瘤农杆菌菌株eha105介导，用构建于质粒pcambia1301的npr1基因转化洋桔梗。
