But there is no reports of the long term organophosphate pesticides contamination on soil microorganisms . more over , in general , the effect of pesticide on soil microbial communities has often been studied by conventional methods based either on cultivation techniques or on metabolic activities 关于有机磷农药长期污染对土壤微生物的影响还未见有报道，而且，一般研究杀虫剂对土壤微生物的影响，都是采用传统的分离培养或测定代谢活力的方法。
The profile of microbial community structures in different deep sea sediments was evaluated and the interaction between microorganisms and environment was analyzed by culture - independent molecular phylogenetic methods . psychrophilic and psychro - tolerant bacteria were cultured and used for screening the cold - active enzymes 此外，本文还对采集自太平洋的深海沉积物和南极、北极区域的样品进行了低温微生物的分离培养及低温酶的筛选与性质分析。
Crumbs of nails were isolated from onychomycosis suffers , koh was chosen to be solvent , nail fungi were examined by microscopy , cultured in sabourud , purified by method of concentration grads and method of exsecting cusp of mycelia . finally , sixteen purified strains were ready 从甲病患者病甲中分离出甲碎屑，选用koh为浮载剂，直接镜检，采用沙堡氏培养基对它们进行分离培养，浓度梯度法和切取菌丝尖端生长法对菌种进行纯化，得到纯化后的菌株16株。
The availability for classification of hemiptera insects by using the esterase isoenzynes , microorganisms and digestive enzymes characters has been studied . the results are as follows . 1 . there were differences in the electrophoretic pattern of the bugs 本文采用酯酶同工酶电泳、镜检与分离培养及消化酶分析对蝽类昆虫酯酶同工酶、体内微生物及主要消化酶类进行了初步研究，结果如下： 1酯酶同工酶电泳表明：蝽类昆虫酯酶同工酶谱存在明显的差异，特征酶谱重复性和稳定性较好。
After 4 days of growth , the intact inner cell mass ( icm ) were separated with 0 . 05 % trypsin - edta and replated on feeder layer in dmem containing 15 % serum , 0 . 1mmol / l nonessential amino acids , 0 . 1mol / l 2 - mercaptoethanol , 2mmol / l glutamine , 100 units / ml of streptomycin and 100 units / ml of penicillin . after 4 - 6 days 5 es cell colonies were selected and expanded in which alkaline alkaline phosphatase was detected ( 2 )小鼠囊胚或内细胞的培养和es细胞的分离培养了156个不同品系的小鼠囊胚，经过3 ? 4天培养后，将增殖出来内细胞团用机械法结合胰酶- edta处理，离散后培养于mef饲养层上， 4 6天后有5例出现了es细胞样集落。
This paper reviews current development in researches of the field of microbial diversity in polluted soils at home and abroad based on the methods of isolating culture and biomarkers , and the knowledge of microbial diversity from the angles of different ecological levels , and explores advanced approaches , new ideas and issues in the research on soil microbial ecological processes aiming at new problems in soil pollution the world is faced with 本文基于分离培养以及生物标志分子方法，从不同生态层次上认识微生物多样性，较全面、系统地综合评述国内外污染土壤环境的微生物群落功能、结构及分子遗传多样性的研究进展，并针对新形势下土壤污染所面临的新问题，探讨了近期土壤微生物生态学过程研究的重要手段与科学问题。
The biological characteristics and toxicity of russula subnigricans hongo was studied for the first time from ecology and morphologic characteristics and histology , the orthogonal experiment of the optimum culture condition , the analysis of components , apoptosis of the cells from little white rat liver and kidney induced by extract of russula subnigricans hongo , to the histopathologic changes observation of little white rat liver and kidney through ecological observation , light microscopy and scanning electron microscopy , reversed - phase high performance liquid chromatography , agarose gel electrophoresis , transmission electron micioscopy . the result showed as below : based on ecological observation of russula subnigricans hongo , its ecological environment was investigated in order to simulate its ecological environment when they are cultivated 利用菌种分离技术、光镜技术、电镜技术、高效液相色谱技术、毒理实验技术、电泳方法等对亚稀褶黑菇( russulasubnigricanshongo )的生物学特性和毒性机理进行了研究，主要包括以下内容：亚稀褶黑菇的生态学和组织学研究、菌种分离培养、扫描电镜观察、成分分析、粗毒液诱导小自鼠肝肾细胞凋亡，小白鼠中毒后肝肾细胞透射电镜观察，研究结果如下： 1
By the compounds of submandibular gland cells and collagen sponges . we investigate the optimal cell denisity of tissue engineered compound of submandibular gland cells and collagen sponges , the cellular compatibility of tissue engineered compound of submandibular gland cells on the collagen sponges with different porosity and the influence of epidermal growth factor on the adherence of submandibular gland cell to collagen sponge . our studies can primary provide theoretical ground work to form the model in vitro of tissue engineering smg 在本研究中，以初步探讨体外颌下腺细胞与胶原海绵支架相互作用为目的，采用体外分离培养sd大鼠颌下腺细胞，然后接种于胶原海绵支架上体外复合培养的方法；从不同接种细胞浓度对细胞一支架复合物影响，同一接种细胞浓度在不同孔隙率的支架上黏附、增殖的情况及表皮生长因子( egf )对颌下腺细胞的促增殖作用，促细胞在支架上黏附等三方面入手，初步研究了颌下腺细胞与胶原海绵相互作用的影响因素，为进一步在体外及体内构建较为理想的组织工程化颌下腺提供理论参数和实验依据。
