Conclusion : neural stem cells maintain their undifferentiated features and have the capacity of self - renewal , proliferation and pluripotentiality 结论：该方法分离培养的神经干细胞保持了干细胞的未分化属性，具有自我更新和多项分化潜能。
Our research work is based on the derivation and cultivation of human embryonic germ cell and mouse embryonic stem cell , and the directed differentiation of mouse embryonic stem cells 本实验也在人类胚胎生殖嵴干细胞、小鼠胚胎干细胞的分离培养，以及小鼠胚胎干细胞的定向分化等方面进行了研究。
The conventional culture - dependent method and culture - independent 16s rdna analysis method were used to investigate the nembure of some soil microbial groups and the bacterial community 同时采用分离培养与不依赖于培养的16srdna分析的方法研究了有机磷农药长期污染对主要土壤微生物生理功能群数量和微生物群落结构的影响。
Invasion of hbmec is essential to the crossing over bbb by e . coli kl . and the invasion process of e . coli to hbmec is a severe stress process . to explore the mechanisms of e . coli kl invasion 人脑微血管内皮细胞( hbmec )是构成血脑屏障的主要成分之一，体外分离培养的hbmec已被视为研究细菌穿过血脑屏障机理的理想模型。
The dna fingerprinting patterns in this study are made to help identification of wild matsutake resources and their tissue isolates under various ecological environments fit for the artificial taming of matsutake 鉴于目前松茸菌丝体分离培养困难，制备松茸子实体的dna指纹图谱，为鉴定不同生境下获得的组织分离物之真伪提供了遗传依据。
Embryonic stem ( es ) cell lines isolated from primitive embryonic cells are highly undifferentiated and totipotent cells . in vitro , es cells can spontaneously differentiate into embryoid bodies ( ebs ) containing derivatives of three germ layers 胚胎干细胞( embryonicstemcell ， escell )系是从早期胚胎细胞分离培养而建立的高度未分化的具有全能性的细胞系。
The kind of fungi fruit bodies develop on recently dead for intrusion or decayed plant materials . the lophodermium and hypoderma fungi collected from the huangshan mountains were isolated by tissue isolation techniques and culture characters data of 7 species were obtained 以组织分离法对采自安徽皖南山区的斑痣盘菌活菌材料进行分离培养，获得7种散斑壳菌及皮下盘菌的培养性状资料。
The results are showed as followed : ( 1 ) the embryo from mouse pregnant 13d , 14d could provide better results in growth states , time of spread layer and lifespan for making and production of murine embryonic body fibroblast cells in our laboratory conditions 研究结果表明： ( 1 )用不同日龄小鼠胚胎( 11 16d )分离培养成纤维细胞，其生长状况、铺层时间及寿命以13d 、 14d小鼠胚胎效果最佳。
( l ) . cultivation of dermal fibroblast : dermal fibroblasts were harvested by using enzyme digest technique from child foreskin after circumcision . 3 - 4 generation of cells were used as work cells . ( 2 ) cultivation of dermal fibroblast on collagen - chitosan spongy membrane 真皮成纤维细胞的分离培养：取包皮环切术后的幼儿包皮酶消化法分离培养真皮成纤维细胞并传代，以3 4代细胞作为工作细胞。
We also investigated the factors including feeder layer cells , growth factors and complements , which may affect the cultivation and differentiation of these cells in vitro . the main contents as follows : ( 1 ) isolation and culture of mouse embryonic fibroblast ( pmef ) and preparation the feeder layers 主要内容包括： ( 1 )小鼠胚胎成纤维细胞( mef )的分离培养和饲养层细胞的制作以妊娠13 . 5天或14 16天的小鼠为材料，建立了mef分离培养体系。
