These results revealed that there may be a complicated interaction among the three signal moleculars no , sa and h2o2 in the responses of arabidopsis to toxin of verticillium dahliae . the interaction played important function in the defence reactions of arabidopsis 综上所述，在大丽轮枝菌毒素与拟南芥幼苗的互作反应中，信号分子no 、 sa和h _ 2o _ 2之间可能存在复杂的相互关系，这种相关性在诱导拟南芥幼苗的防卫反应中起重要的调控作用。
Two primers , designed according to the conserved regions of ban gene in arabidopsis thaliana , were used to amplify the ban homologous fragments from the genomic dna of brassica napus , b . chinensisl , b . juncea , a . thaliana and other cultural plants of cruciferae . the very similar pcr fragments were obtained from all the amplifications , which implicated that ban may be a conserved gene existing widely in the genomes of cruciferae . pcr fragments were cloned and confirmed by restriction enzyme digestion 参照拟南芥( arabidopsisthaliana ) ban基因与cdna设计引物，对甘蓝型、白菜型、芥菜型油菜，拟南芥及其它十字花科栽培品种的基因组dna进行pcr扩增，均扩增出与拟南芥ban基因扩增片段大小极其相似的dna片段，提示ban可能广泛存在于十字花科植物中。
Homology analysis blast analysis showed that the amino acid sequence of the asf gene cloned from cotton was significantly homologous with adp - ribosylation factor ( asf ) of mammalian , plant and yeast , etc . the amino acid sequence of the gene shows 99 % ( 179 / 180 ) , 98 % ( 177 / 180 ) , 96 % ( 174 / 180 ) and 92 % ( 168 / 180 ) identity to arabidopsisthalianaarfl , triticum aestivum , solatium tuberosum and bos taurus , respectively 棉花arf基因的同源性分析应用ncbi进行dna序列的相似性分析，结果表明：棉花arf基因与其它植物、动物和酵母的arf基因具有较高的同源性。棉花arf基因编码的氨基酸序列与拟南芥，小麦、马铃薯、牛的同源性分别达到99 ( 179 / 180 ) 、 98 ( 177 180 ) 。 96 ( 174 180 )杨花肠p核符吞fi曰手谷回伽皿的夭险和92 （ 168ill ） 。
The sequences of intron , however , had only 74 % of homology with those of a . thaliana ban . in addition , amino acid sequence of oilseed rape ban had 78 % of homology and 87 % of similarity with those of a . thaliana and had various homologies ( 55 - 64 % ) with many nadph - dependent reductases of other plants 将甘蓝型油菜ban氨基酸序列进行同源比较，发现甘蓝型油菜ban片段的181个氨基酸与拟南芥有78的氨基酸序列相同，相似性达87 ；与80多种植物的nadph还原酶类也有不同程度的同源性( 55 - 64 ) 。
The dual effects of no were validated in this research . no was either oxidative or antioxidative which depends on the circumstances . generally no took part in the antioxidant cellular system by elevating the activities of cat and apx . while in the vd - toxin infected arabidopsis , no supressed the activity of cat and may participate in redox signaling during the activation of defense responses which follows the pathognen attack 本实验结果进一步验证了no作用的双重性，即在通常情况下， no表现出的是抗氧化性，而当毒素与拟南芥互作时， no通过抑制cat活性，促进h _ 2o _ 2的积累，传递抗病信号，表现其氧化性的一面。
Lfy and lfy - like proteins from angiosperm contain a proline - rich region near the amino terminal and an acidic central region which is typical for transcriptional activators and may be important for the function of lfylike protein . the two domain exists in the variable regions of all lfy - like proteins 与拟南芥leaf ’ y基因相比较，银杏ginlfy和g毗基因没有做为转录激活区域的位于氨基酸序列中间的酸性区和位于n端的富含脯氨酸区，但这两个区域均位于leafy基因的非保守区。
In this experiment , seedlings of arabidopsis thaliana ( col ) were observed after being treated by verlicillium dahliae ( vd - toxin ) , exogenous salicylic acid ( sa ) , nitric oxide donor ( snp ) and nitric oxide synthase inhibitor ( nna ) , then we investigated the changes of endogenous h2o2 content , the activity of the antioxidant enzymes catalase ( cat , ec : 1 . 11 . 1 . 6 ) and ascorbate peroxidase ( apx , ec : 1 . 11 . 1 . 11 ) and mrna levels of cat3 in different stress conditions , we also identified the localizations of h2o2 and no accumulated in the leaves of arabidopsis 本实验研究了棉花黄萎病菌?大丽轮枝菌毒素( vd - toxin )与拟南芥幼苗互作反应中外源sa 、 no供体snp 、 no合酶抑制剂nna等不同处理对拟南芥幼苗h _ 2o _ 2含量、 cat和apx活性及cat基因mrna表达量的影响，并对no 、 h _ 2o _ 2的积累部位进行染色检测。
The activity of apx was also decresed after sa and vd - toxin treatment . the activity of cat was decreasded more obviously . in the vd - toxin infected arabidopsis , no also restrained the activity of cat and decreased the level of cat3 mrna . the results suggested that h2o2 acted as a signal to involve the defense reactions which is induced by sa and no in arabidopsis No在毒素与拟南芥的互作过程中也表现出对cat的抑制作用，结果表明， h _ 2o _ 2可能作为信号分子参与了sa和no调控的拟南芥的防卫反应， sa和no或许在转录和蛋白水平上通过对cat的抑制作用而促进h _ 2o _ 2的积累。
The clones inserted with ban fragments of b . napus and a . thaliana were sequenced and compared with the sequences in genbank . the results showed that the ban fragment of a . tha liana had the same sequence ( 779bp ) as reported . the ban fragment of b . napus was 780bp and its extron had 90 % of homology with those of a . thaliana ban ( 90 % ) Pcr产物经克隆测序和比较分析显示，拟南芥ban片段( 779bp )与已报道的完全相同，甘蓝型油菜ban片段( 780bp )与拟南芥ban外显子的同源性为90 ，但内含子的同源性仅为74 。
Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ) , which encoded a protein consisting of 524 aa with molecular weight of 62 . 2 kda and pi of 8 . 96 . strongly basic ( + ) amino acids , strongly acidic ( - ) amino acids , hydrophobic amino acids and polar amino acids of the protein were 13 . 74 % , 11 . 64 % , 36 . 45 % and 22 . 70 % respectively , and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site , protein kinase c phosphorylation site , casein kinase ii phosphorylation site , n - myristoylation site , camp - and cgmp - dependent protein kinase phosphorylation site , tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450 . a - helix and b - sheet of the protein is 47 . 7 % , 45 . 0 % respectively Huangya14 )为材料分离克隆到一个细胞色素p450基因，命名为bccyp86mf5 ， cdna全长1854bp ，含1575bp的完整开放阅读框，编码524个氨基酸，其编码蛋白质的分子量为61 . 2kda 、等电点为8 . 96 ；碱性氨基酸、酸性氨基酸、疏水氨基酸和极性氨基酸分别占总氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ；二级结构预测包括n -糖基化位点、依赖于camp和cgmp的蛋白激酶磷酸化位点、蛋白激酶c磷酸化位点、酪蛋白激酶磷酸化位点、酪氨基酸激酶磷酸化位点、 n -豆蔻酰化位点和细胞色素p450的典型区域，半胱氨酸亚铁血红素配体信号区等， -螺旋和-折叠分别占47 . 7 、 45 . 0 ；与bccyp86mf1基因的氨基酸序列同源性达到95 . 2 ，与拟南芥cyp86c4的达到85 . 9 。
