The crosslinking products of several bifunctioanal crosslinkers with different length of arm spacer and different solubility to water or lipid were detected by sds - page and then western blotting or two - dimension electrophoresis . it was found that 33 kd protein can be crosslinked with cp47 , cp43 or d2 by edc ; cp47 dimer and crosslinking between cp43 and d2 occur when egs was applied ; treatment of psil core complex with dtsp turn out the products from 33 kd protein , di and the components of lhcii , those comprised by di and the components of lhcii , and those constituted by cp29 , psb s and lhcii . the above results suggest that the protein subunits existing in the same crosslinking products are located closely in the psil core complx , which indicate the relationship of their functions 结果显示： 33kd蛋白能与cp47 、 cp43和dz蛋白交联；在交联剂egs作用下cp47形成二聚体和发生dz与cp43的交联；在dtsp处理下， 33kd蛋白、 di蛋白、 cp29和部分lhcll组分形成110kd左右的交联带， d ；与部分lhcll组分组成了55kd交联带， cp29 、 psbs及部分lhcll组分组成了45kd交联带c说明上述psll放氧核心复合物蛋白组分在空间位置上的邻近关系，也暗示了它们在功能卜的联系。
