In both cell lines ex - pressing sh2a gene , the cytoplasm pkc activity decreased 40 % , but no apparent alteration was found in mapk , tpk and membrane pkc activity . the proliferation index of transfected cells exhibited an decline in cytometry detection L sh的肝癌bel7402细胞及cos7细胞胞浆蛋白中的pkc活性均下降达40 ，胞膜pkc 、胞浆mapk ， ’ fpk活性未见明显改变，转染细胞经流式细胞仪检测增殖指数有所下降。
Section 2 flow cytometry combined assay for phosphatidylserine and cd62pexpressed by preserved platelets human platelets have distinct characters when preserved by different methods . a efficient cytometric assay for different preserved platelets expression of cd62p and phosphatidylserine ( ps ) is in dire need 第二节，流式细胞术联合测定保存血小板表达的磷脂酞丝氨圈和cd62p人类血小板通过不同的技术进行离体保存后，其特性改变差异较大。
The dynamic changes of cd4 and cds in the pbmc after challenged with prrsv were measured by flow cytometry . the cd4 * and cds * t lymphocytes in blood of porcine infected with prrsv were marked by anti - cd4 * and cds * t lymphocytes monoclonal antibodies , and then detected by flow cytometry 利用流式细胞仪检测猪感染prrsv以后不同时期cd4和cd8动态变化的研究，从猪心脏采血，分离外周血淋巴细胞，采用吉姆萨染色计数，单抗标记，然后进行流式分析。
To facilitate supervision and management of food production and circulation , rapid methods for detection of foodborne pathgens , such as improvement and automatization of conventional methods , bioluminescent method , cytometry , impedancemetry and immunological methods were reviewed 为方便对食品生产和流通的整个过程进行卫生监督和管理,就常规方法的改进和自动化、生物发光方法、细胞计数方法、阻抗测定方法和免疫学方法等食品中微生物的快速检测方法进行了综述。
In the first trial , combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis , and then a modified discontinuous percoll gradient centrifugation method ( 15 % , 22 % , 30 % , 40 % , 50 % , 60 % ) was introduced to isolate spermatids from the cellular suspensions . the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis , and the viability of spermatogenic cells was assessed by using eosin y exclusion test 在第一部分试验中，首先利用连续3次组合酶消化成年小鼠睾丸制备睾丸细胞悬液，然后经6层非连续percoll梯度离心法( 15 、 22 、 30 、 40 、 50和60 )分离，通过形态学和流式细胞术鉴定南京医科大学硕士学位论文各个percoll组分中精子细胞的含量，并以伊红y排斥试验测定细胞的存活率。
Analysis of synchronization - treated bear fibroblast cells cycle by flow cytometry after 3 passage of the primary black bear fibroblast cells , the cells were serum starvated for 2 - 6 days or treated with 10g / ml nocodazole for 24h , then calculation of percentages of the treated cells and the control cells in g0 , g1 , and g2 + m phases of the cell cycle by using flow cytometry , respectively . go + g1 cell percentage of the serum starvation group , nocodazole treatment group and control group are 85 . 05 + 6 . 7 , 45 . 9 + 3 . 7 and 59 . 3 + 6 . 7 , respectively 流式细胞仪分析结果表明，血清饥饿处理能较好地使黑熊成纤维细胞同步于g _ 0和g _ 1期，其中饥饿3d时， g _ 0 + g _ 1期的细胞所占百分率最高，达到( 85 . 05 7 . 0 ) ；而nocodazole处理第四军医大学硕士学位论文则使细胞较大程度同步于g 。
Thus , in order to investigate the developmental pathways not only involved in the regulation of growth and patterning , but also in the determination of cell lineages and differentiation , we utilized the fluorescent immunohistochemical methods , flow cytometry analysis sorting ( facs ) and molecular methods to investigate the developmental law of mammary gland at the different developmental stages , distribution of the stem cells in mammary gland , the methods of isolation , culture and evaluation for the stem cells , the multipotent abilities in vivo and in vitro , and the efficient cultural system for stem cells enriched in vitro . the results showed below : 1 我们以小鼠为模型，运用组织化学、免疫荧光组织(细胞)化学、流式细胞仪分选方法( facs )以及分子生物学手段，研究了小鼠乳腺的发育规律：小鼠乳腺组织中类乳腺干细胞：小鼠乳腺细胞的分离、培养以及类乳腺干细胞的鉴定；小鼠类乳腺干细胞分化的潜能；小鼠乳腺类腺体体外短期培养富集类乳腺干细胞体系的优化等。研究结果表明： 1
In this work , effects of sfamnpv on mitochondrial and er in sl - 1 cells were studied . the main results were as follows : effect of sfamnpv ( moi = 6 ) on the function of mitochondrial in sl - 1 cells was investigated by mtt assay and membrane potential was assayed by both flow cytometry and confocal laser scanning microscope 完成的工作包括以下几个方面： ( 1 )细胞内线粒体的变化通过mtt法研究病毒感染对细胞线粒体功能的影响，同时用流式细胞仪和共聚焦激光显微镜研究线粒体膜电位( m )的变化， western检测2种凋亡调控蛋白cytoc和bcl - 2蛋白的变化。
Surface markers on dcs were then analyzed by flow cytometry and the proliferation of t cells was detected by mtt colorimetry . resoults : peripheral blood monocytes from patients of carcinoma treated with rhgm - csf of 1000 u / ml plus il - 4 of 500 u / ml for 7 days could observe dcs with typical morphology . simultaneously there was a decrease in cd 14 expression and increase in hla - dr , cd40 , cd83 and cd86 on dcs 结果，癌症患者外周血中的单核细胞在rhgm - csf1000u ml + il - 4500u ml的条件下培养一周，就可看到典型的树突状细胞形态，其表面cd14分子表达减少， hla - dr 、 cd54 、 cd40 、 cd83及cd86分子的表达明显增高，且具有明显刺激t细胞增殖的能力，成功地完成了外周血单核细胞来源的dc的培养。
In the second trial , this modified discontinuous percoll gradient centrifugation method was introduced to isolate spermatids from the semen of fifteen male infertile patients . then the effect was identified by wright - giemsa stain , flow cytometry analysis , immunocytochemistry and fluorescence in situ hybridization ( fish ) . similary , the 22 % percoll fraction contained mostly haploid cells [ ( 91 . 85 ? 5 . 18 ) % ] ( p < 0 . 005 ) and the mean density in this fraction was ( 1 . 010 ? 0 . 786 ) x 105 / ml C法，对15例各种类型不育患者的精液细胞进行分离，并利用瑞姬染色法、流式细胞术、免疫细胞化学和荧光原位杂交oisffi等方法，从细胞形态特征、 dna倍体、细胞表面标i己与分化抗原，以及原位杂交信号的数目和位置结合细胞核特有的形态等方面加以鉴定。
