Methods : flow cytometry and proteomics were used respectively to measure the effects of ligustrazine on cell cycle and protein expression of hfcl 方法：分别采用流式细胞术和蛋白质组学方法测定川芎嗪对人骨髓成纤维样基质细胞周期及蛋白质表达的影响。
The decline of proliferation index of transfected cells in cytometry detection also demonstrated that sh2a gene has an inhibitory effect in cell proliferation 通过流式细胞仪检测sh基因对细胞增值的影响，结果显示转染sh基因使细胞的增殖指数下降，表明sh基因对细胞的增殖有一定抑制作用。
Apoptosis was identified by dna electrophoresis and flow cytometry . the results showed the supernatant containing hsblys induced a does and concentration - dependant increase in apoptosis cells Dna琼脂糖凝胶电泳和流式细胞仪结果显示， hsblys表达上清可诱导k562细胞凋亡，且具有剂量依赖效应。
This was achieved by using flow cytometry to simultaneously measure cellular dna and protein content , enabling the calculation of percentages of cells in g0 , g1 , s and g2 + m phases of the cell cycle 通过流式细胞仪来同时检测细胞的dna和蛋白质的含量，这样就能够计算出细胞处于g0期， g1期， s期以及g2期+ m期的百分率。
Cell injury was investigated by lactate dehydrogenase ( ldh ) activity in culture medium . and cell apoptosis was examined by flow cytometry , tunel staining and transmission electron microscopy ( tem ) 重度低氧（ 2 3 oz ） 24h ，心肌细胞数和蛋白质合成均降低，并且培养液中ldh水平增高，提示重度低氧有细胞毒性作用。
The supernatant containing hsblys co - stimulated b cell proliferation highly with anti - igm by mtt analysis . flow cytometry results showed only in the function of costim lation , can b cell entered the s phase of cell cycle Mtt法知hsblys表达上清对b淋巴细胞具有共刺激作用；流式细胞仪分析显示只有在共刺激作用下， b细胞才进入s期。
Northern analysis result indicated that a - 1bgp could be stably expressed in transfected cell . the transfected cells showed increase in s phase and pi index and displayed decrease in g0 / g1 phase by flow cytometry analysis Ibgp及ppic加质粒转染毕式酵母gslls菌株，提取gslls a叫酵母dna ， pcr扩增，电泳结果表明a则已有效地整合酵母基因组中。
0 . pc dna3 . 1 - a - 1bgp vector was constructed and transfected into hepatoma cells ( bel - 7402 ) using calcium - phosphate coprecipitation , then cell dna content was analyzed by flow cytometry L a ibgp的真核细胞表达载体，并用磷酸钙共沉淀法转染细胞， northern印迹杂交分析转染后a ibgp的表达，流式细胞仪分析细胞周期，检测ajbgp对细胞增殖的影响。
The fetal liver stem cells were isolated by collagenase digestion , gravity sedimentation and density gradient centrifugation , identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition 采用胶原酶消化、重力沉降及密度梯度离心方法分离人胎肝干细胞，通过免疫细胞化学方法对其进行初步鉴定，以及应用流式细胞仪等对其生长状况进行评估。
Flow cytometry measurements were done to detect the changing of fluorescent signal of the reporter strain and give expression situation to ybt indirectly . 7 eaggec hpi - positive strains revealed an enhanced fluorescence signal but 1 eaggec hpi - negative did not so 3n ） ，将待测eaggec菌株的培养上清加入该报告菌株培养物中，用流式细胞术facs方法检测报告菌株荧光强度的变化情况，间接反映ybt的表达与否。
