It is a famous and costly medicinal fungus , and it was investigated clearly in its biological feature and chemical components . now cordyceps sinesis ( berk . ) sacc can be produced by unnatural cultivation , not native collection , which brings many advantages of its output and application 本课题以提高冬虫夏草菌丝体的主要活性化学成分腺苷的含量为目的，对冬虫夏草cordycepssinensis ( berk . ) sacc .菌株cs - js进行了菌种选育和发酵的研究，并初步建立了冬虫夏草发酵菌丝体的毛细管电泳指纹图谱，为冬虫夏草菌丝体产品的研发和产品的鉴别打下了基础。
A water soluble crude polysaccharide in the cells has been isolated from the zymogen thread of hericium erinaceus pers . after the crude polysaccharide has been deamylumized by the combined methods of enzyme and sevage , the four graded component - hpa , hpb , hpc , hpd - would be abstracted by the method of ethanol grading 从猴头菌( hericiumerinaceuspers )发酵菌丝体中提取出胞内水溶性粗多糖，经酶法和sevage法联合脱蛋白后，用乙醇分级法分离出hpa 、 hpb 、 hpc 、 hpd四种级分。
In order to devolping and utilizing phellinus igniarius resources , the main biologic characteristics and polysaccharide of phellinus igniarius were studied . meanwhile the optimum method suitable for mass production of polysacchride was established . the basis data on studying active compositions , developing new medicine and culturing fruit bodies from mycelia of phellinus igniarius were provided 本文以开发利用桑黄这一新兴的药用菌物资源，探明其菌丝体生长条件、活性成分为目的，对桑黄的主要生物学特性进行测定，对其主要活性成分多糖进行分析，以正交试验确定桑黄菌丝体多糖的提取工艺，为桑黄的活性成分研究、开发新药及人工栽培桑黄子实体提供了科学依据。
The fungus age , enzyme system , osmotic stabilizer , ca2 + concentration influenced on the formation of the protoplast have been confirmed . the result show that 16 - 18hr fermention , 32 1 . 0 % cellulase plus 1 . 0 % snailase confected by pba ( phosphate blend ammonium chloride ) contant 0 . 2 % ca2 + , acted 3 hr is optimal for the protoplast production of aspergillus . niger 分别采用单因子法和正交实验法考查，认为16 18hr菌龄的菌丝体， 32 ,用含0 . 2 ca ~ ( 2 + )的pba高渗缓冲液配制的1纤维素酶与1蜗牛酶的混合酶酶解3hr ，得到的原生质体数最多。
The biological characteristics of mycelia from phellinus igniarius and culture media were studied . two kinds of culture media were suitable for the growth of mycelia . the result indicated that the culture medium with potato as nitrogen source and saccharose as carbon source was suitable for collecting mycelia , and the culture medium with peptone as nitrogen source and solvable amylum as carbon source was suitable for conservation 为了最大限度地保存菌种的活力，以提高菌丝体的质量及菌丝体内活性成分的累积，本文通过对比研究，进一步对其生长基质进行筛选，明确了两种适于桑黄菌丝生长的固体培养基：以马铃薯为氮源、蔗糖为碳源的培养基较适用于菌丝收集，以蛋白胨为氮源、可溶性淀粉为碳源的培养基较适用于菌种的保藏。
Polysaccharide of mycelia from phellinus igniarius cultured by shaking were studied . lg9 ( 34 ) orthogonal experiment were carried out to find the optimum method of extracting polysaccharide from mycelia . the magnitude of range indicated that the factors which effected extracting ratio were times , time , temperature and proportion 本文对液体培养桑黄菌丝体的主要活性成分多糖进行了研究，首次利用lg9 ( 3 ~ 4 )正交试验法对桑黄菌丝体多糖的提取工艺进行研究，从级差的大小可以看出影响桑黄菌丝体粗多糖提取率的因素主要是浸提次数，其次分别是浸提时间、吉林农业大学硕士学位论文桑黄主要生物学特性及多糖的研究浸提温度、浸提比。
The rapd - pcr analyses were conducted with different colour basidiocarps from different origin pleurotus mushrooms . the results showed that there are same dna fingerpringing patterns among the mycelial isolates , pileus ( including lamellae ) and stipe from one basidiocarps , but the similarity coefficients between different origin and colour basidiocarps vary from 0 . 779 to 0 . 976 试验结果还表明：人工栽培产生的子实体及其组织分离菌丝体，与所用栽培菌种茵丝体及其种源子实体，都具有一致的rapd指纹图谱：而不同来源的子实体之间的dna相似系数在0 . 886 0 . 986之间。
We controlled the produce process and drug standard by advanced methods . the bacterio species was isolated from cryptoporus sinensis sheng h . wu & m . zang growing widely in yunnan province , which was suited to be submerged cultivation and grow quickly , and cultured by fermentation in liquid . the fermentation technique has already applied for nation patent and could produce mass high quality mycelium and metabolite stably 本课题所用的菌种是最初从云南野生中华隐孔菌( cryptoporussinensisshengh . wu & m . zang )于实体中分离培养获得的一株适合于液体深层培养，生长速度快的菌种，通过已申请国家专利的液体培养发酵技术，能稳定地获得大量、高质量的发酵菌丝体及其代谢产物。
A substance extracted from mycelia with benzinum was studied for the first time . five compositions were found : butylated hydroxytoluene , n - hexadcanoic acid , 9 , 12 - octadecadienoic acid methyl ester , eicosane and hexacosane . the paper provided scientific basis data for researching mainly biologic characteristics and active compositions of mycelia from phellinus igniarius cultured with solid and liquid culture media 首次对菌丝体的石油醚提取物进行定性分析，确定该菌丝体中含有二丁基夯基甲苯、棕桐酸、 9 ， 12一十八碳二烯酸甲醋，二十碳烷及二十六碳烷，这五种物质均为在桑黄菌丝体中首次发现。
